ERGIC2 Antibody

Datasheet

Code	CSB-PA857020EA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg), 786-O whole cell lysate(30µg), U251 whole cell lysate(30µg), CACO2 whole cell lysate(30µg), A431 whole cell lysate(30µg),U87 whole cell lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Brain tissue lysate(30µg) All lanes: ERGIC2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 120s IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ERGIC2 Polyclonal antibody

Uniprot No.

Q96RQ1

Target Names

ERGIC2

Alternative Names

ERGIC2; ERV41; PTX1; CDA14; Endoplasmic reticulum-Golgi intermediate compartment protein 2

Raised in

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human ERGIC2. AA range:138-200

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Possible role in transport between endoplasmic reticulum and Golgi.

Gene References into Functions

A variant of ERGIC2 with four coding bases deleted has an abrogated function as a transport shuttle, but does continue to upregulate the heme oxygenase 1 gene, suggesting that it may be involved in the oxidative stress pathway. PMID: 24303950
may play an important role in the growth and tumorigenicity of PC-3 prostate tumor cells PMID: 12932305
ERGIC-32 functions as a modulator of the hErv41-hErv46 complex by stabilizing hErv46 PMID: 15308636
Ectopic expression of a partial sequence of PTX1 (Met84 - Leu225) as a VP22-fusion protein in prostate cancer cell line, PC-3, induced cellular senescence. PMID: 16989575
CDA14 participated in the elongation factor 1alpha regulated mechanisms PMID: 17980171

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Subcellular Location

Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. Nucleus.

Protein Families

ERGIC family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 30208

OMIM: 612236

KEGG: hsa:51290

STRING: 9606.ENSP00000353270

UniGene: Hs.339453

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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