FBXO3 Antibody

Datasheet

Citations (1)

Code	CSB-PA892132LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U251 whole cell lysate(30µg), A431 whole cell lysate(30µg), A549 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg),Mouse Liver tissue lysate(30µg),Mouse Lung tissue lysate(30µg),Mouse Kidney tissue lysate(30µg) All lanes: FBXO3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 55,48,47 kDa Observed band size: 55 kDa Exposure time: 60s
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FBXO3 Polyclonal antibody

Uniprot No.

Q9UK99

Target Names

FBXO3

Alternative Names

F Box G - Domain 3 antibody; F box only protein 3 antibody; F box protein 3 antibody; F box protein FBX3 antibody; F-box only protein 3 antibody; FBA antibody; FBX3 antibody; FBX3_HUMAN antibody; FBXO3 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human FBX3

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

E3 Ubiquitin Ligase FBXO3 Drives Neuroinflammation to Aggravate Cerebral Ischemia/Reperfusion Injury Y Gao,International journal of molecular sciences,2022

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Immunofluorescence (IF) Analysis

Sample type: cells

Review: Treatment of si-FBXO3 inhibited inflammatory response induced by ischemia/reperfusion (I/R) injury in vivo.

By Anonymous

Target Background

Function

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.

Gene References into Functions

we report the X-ray structure of the human FBxo3 ApaG domain, residues 278-407, at 2.0 A resolution PMID: 27010866
AIRE, which is phosphorylated on two specific residues near its N terminus, then binds to the F-box protein 3 (FBXO3) E3 ubiquitin ligase. In turn, this SCF(FBXO3) (SKP1-CUL1-F box) complex ubiquitylates AIRE, increases its binding to the positive transcription elongation factor b (P-TEFb), and potentiates its transcriptional activity. PMID: 27365398
Fbxo3 promotes the proteasomal degradation of Smurf1. Fbxo3 promotes the poly-ubiquitination of Smurf1. PMID: 25721664
Virulence factor NSs of rift valley fever virus recruits the FBXO3 to degrade subunit p62 of general transcription factor TFIIH. PMID: 24403578
controls TRAF adaptor stability to regulate inflammation PMID: 23542741
Fbx3 formed SCF(Fbx3) ubiquitin ligase and promoted the degradation of HIPK2 and p300 by the ubiquitin-proteasome pathway PMID: 18809579

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Subcellular Location

Nucleus. Note=Colocalizes with PML at the peripheries of nuclear bodies.

Database Links

HGNC: 13582

OMIM: 609089

KEGG: hsa:26273

STRING: 9606.ENSP00000265651

UniGene: Hs.406787

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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