FBXO3 Antibody

Code CSB-PA892132LA01HU
Size US$299
Image
  • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA892132LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA892132LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Western Blot
    Positive WB detected in: Mouse kidney tissue
    All lanes: FBXO3 antibody at 4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 55, 48, 47 kDa
    Observed band size: 55 kDa

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) FBXO3 Polyclonal antibody
Uniprot No. Q9UK99
Target Names FBXO3
Alternative Names F Box G - Domain 3 antibody; F box only protein 3 antibody; F box protein 3 antibody; F box protein FBX3 antibody; F-box only protein 3 antibody; FBA antibody; FBX3 antibody; FBX3_HUMAN antibody; FBXO3 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human F-box only protein 3 protein (276-376AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The FBXO3 Antibody (Product code: CSB-PA892132LA01HU) is Non-conjugated. For FBXO3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA892132LB01HU FBXO3 Antibody, HRP conjugated ELISA
FITC CSB-PA892132LC01HU FBXO3 Antibody, FITC conjugated
Biotin CSB-PA892132LD01HU FBXO3 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Customer Reviews and Q&A

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Applications : Immunofluorescence (IF) Analysis

Sample type: cells

Review: Treatment of si-FBXO3 inhibited inflammatory response induced by ischemia/reperfusion (I/R) injury in vivo.

By Anonymous

Target Background

Function
Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
Gene References into Functions
  1. we report the X-ray structure of the human FBxo3 ApaG domain, residues 278-407, at 2.0 A resolution PMID: 27010866
  2. AIRE, which is phosphorylated on two specific residues near its N terminus, then binds to the F-box protein 3 (FBXO3) E3 ubiquitin ligase. In turn, this SCF(FBXO3) (SKP1-CUL1-F box) complex ubiquitylates AIRE, increases its binding to the positive transcription elongation factor b (P-TEFb), and potentiates its transcriptional activity. PMID: 27365398
  3. Fbxo3 promotes the proteasomal degradation of Smurf1. Fbxo3 promotes the poly-ubiquitination of Smurf1. PMID: 25721664
  4. Virulence factor NSs of rift valley fever virus recruits the FBXO3 to degrade subunit p62 of general transcription factor TFIIH. PMID: 24403578
  5. controls TRAF adaptor stability to regulate inflammation PMID: 23542741
  6. Fbx3 formed SCF(Fbx3) ubiquitin ligase and promoted the degradation of HIPK2 and p300 by the ubiquitin-proteasome pathway PMID: 18809579

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Subcellular Location Nucleus. Note=Colocalizes with PML at the peripheries of nuclear bodies.
Database Links

HGNC: 13582

OMIM: 609089

KEGG: hsa:26273

STRING: 9606.ENSP00000265651

UniGene: Hs.406787

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