FBXO4 Antibody

Datasheet
Code CSB-PA891538ESR1HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q9UKT5
Image
  • Western blot
    All lanes: FBXO4 antibody at 3.1μg/ml
    Lane 1: 293T whole cell lysate
    Lane 2: HepG2 whole cell lysate
    Lane 3: HL60 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 45, 36 kDa
    Observed band size: 45 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA891538ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA891538ESR1HU at dilution of 1:100

  • Immunoprecipitating FBXO4 in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA891538ESR1HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
    Lane 2: CSB-PA891538ESR1HU (4µg) + HepG2 whole cell lysate (500µg)
    Lane 3: HepG2 whole cell lysate (20µg)

Immunogen Recombinant Human F-box only protein 4 protein (138-387AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Relevance Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias F-box only protein 4, FBXO4, FBX4
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Research Area Cell Biology
Target Names FBXO4
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
Subcellular Location Cytoplasm
Database Links

HGNC: 13583

OMIM: 609090

KEGG: hsa:26272

STRING: 9606.ENSP00000281623

UniGene: Hs.165575

Pathway Ubiquitin mediated proteolysis

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