FEV Antibody

Datasheet

Code	CSB-PA858717LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HL60 whole cell lysate, Rat brain tissue All lanes: FEV antibody at 5.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa IHC image of CSB-PA858717LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA858717LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA858717LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FEV Polyclonal antibody

Uniprot No.

Q99581

Target Names

FEV

Alternative Names

ETS-domain transcription factor antibody; FEV (ETS oncogene family) antibody; FEV antibody; FEV ETS transcription factor antibody; FEV_HUMAN antibody; Fifth Ewing sarcoma variant antibody; Fifth Ewing variant protein antibody; HSRNAFEV antibody; mPet1 antibody; PC12 ETS domain-containing transcription factor 1 antibody; PC12 ETS factor 1 antibody; Pet-1 antibody; Protein FEV antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Protein FEV protein (1-97AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FEV Antibody (Product code: CSB-PA858717LA01HU) is Non-conjugated. For FEV Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA858717LB01HU	FEV Antibody, HRP conjugated	ELISA
FITC	CSB-PA858717LC01HU	FEV Antibody, FITC conjugated
Biotin	CSB-PA858717LD01HU	FEV Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.

Gene References into Functions

This study adds to the growing evidence on the association of single- and multiple-risk variants in DRD3, DRD4, and FEV with aggressive behavior in Chinese adolescents. PMID: 28846959
identified FEV is unique to fetal HSCs and stably expressed in leukemic cells of prenatal origin PMID: 27807368
Genetic variation in FEV1 was associated with serum 25-Hydroxyvitamin D status. PMID: 26122139
novel evidence for the role of Pet-1 in human amygdala threat processing extends literature demonstrating the influence of genetic variation in the serotonin system on emotional regulation PMID: 24100022
Fev-ERK signaling is essential for hemogenic endothelium-based hematopoietic stem cell development. PMID: 23591790
our data argue against an exclusive role of FEV in the adult human brain serotonergic system and genetic analyses did not suggest that FEV variation adds to the genetic liability towards affective disorders PMID: 20480378
FEV identifies serotonin-producing cells in normal and neoplastic small intestine. PMID: 20048018
Serotonin-related FEV gene variant in the sudden infant death syndrome is a common polymorphism in the African-American population. PMID: 19707175
FEV acts as a transcriptional repressor through its DNA-binding ETS domain and alanine-rich domain. PMID: 12761502
We showed that fev is exclusively expressed in the midline part of the human brainstem containing raphe nuclei, which also specifically expressed 5-HT transporter (sert) and tryptophan hydroxylase (tph), two markers of the 5-HT neurotransmitter system. PMID: 15003288
Analysis of transgene expression in Pet-1 null mice indicates that Pet-1 is required to maintain the activity of the Pet-1 enhancer region in a subset of serotonin (5-HT) neurons. PMID: 15758173
Variation in FEV gene observed exclusively in a subset of African American sudden infant death syndrome cases may help explain the observed abnormalities of this system in some SIDS cases and the ethnic disparity. PMID: 17597646

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Involvement in disease

Sudden infant death syndrome (SIDS)

Subcellular Location

Nucleus.

Protein Families

ETS family

Tissue Specificity

In brain, exclusively expressed in the major serotonergic neurons of the dorsal and median raphe nuclei located in the midbrain and pons. Also detected in prostate and small intestine.

Database Links

HGNC: 18562

OMIM: 272120

KEGG: hsa:54738

STRING: 9606.ENSP00000295727

UniGene: Hs.234759

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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