FRMD6 Antibody

Datasheet

Code	CSB-PA822270ESR2HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA822270ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human duodenal tissue using CSB-PA822270ESR2HU at dilution of 1:100 Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: FRMD6 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 71, 30 kDa Observed band size: 73 kDa IHC image of CSB-PA822270ESR2HU diluted at 1:293 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FRMD6 Polyclonal antibody

Uniprot No.

Q96NE9

Target Names

FRMD6

Alternative Names

FRMD6 antibody; C14orf31FERM domain-containing protein 6 antibody; Willin antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human FERM domain-containing protein 6 protein (413-622AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

a novel function of FRMD6 in inhibiting human GBM growth and progression and uncover a novel mechanism by which FRMD6 exerts its anti-GBM activity. PMID: 27661120
Genome-wide and gene-based association implicates FRMD6 in Alzheimer disease. PMID: 22190428
Human homolog of Drosophila expanded, hEx, functions as a putative tumor suppressor in human cancer cell lines independently of the Hippo pathway PMID: 21785462
in mammalian cells willin influences Hippo signaling activity by activating the core Hippo pathway kinase cassette PMID: 21666719
Results describe a new FERM domain-containing protein called Willin. PMID: 16137681

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Can colocalize with actin.

Database Links

HGNC: 19839

OMIM: 614555

KEGG: hsa:122786

STRING: 9606.ENSP00000348550

UniGene: Hs.434914

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Hippo Signaling Pathway Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Hippo signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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