FRMD6 Antibody

Datasheet
Code CSB-PA822270ESR2HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q96NE9
Image
  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA822270ESR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human duodenal tissue using CSB-PA822270ESR2HU at dilution of 1:100

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
    All lanes: FRMD6 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 73, 71, 30 kDa
    Observed band size: 73 kDa

  • IHC image of CSB-PA822270ESR2HU diluted at 1:293 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Recombinant Human FERM domain-containing protein 6 protein (413-622AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias FERM domain-containing protein 6 (Willin), FRMD6, C14orf31
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cancer
Target Names FRMD6
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Cytoplasm, Cell membrane, Peripheral membrane protein, Cytoplasmic side
Database Links

HGNC: 19839

OMIM: 614555

KEGG: hsa:122786

STRING: 9606.ENSP00000348550

UniGene: Hs.434914

Pathway Hippo signaling pathway

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