Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA822270ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human duodenal tissue using CSB-PA822270ESR2HU at dilution of 1:100
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
All lanes: FRMD6 antibody at 3µg/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73, 71, 30 kDa
Observed band size: 73 kDa
IHC image of CSB-PA822270ESR2HU diluted at 1:293 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.