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Product Name:

Formyl-HIST1H4A (K12) Antibody

Code : CSB-PA010429OA12forHU
Size : US$319
Uniprot NO. : P62805
Abbreviation : Histone H4
Storage : Upon receipt,store at -20°C or -80°C.Avoid repeated freeze.
Image :
  • Western Blot
    Positive WB detected in:293 whole cell lysate(treated by 30mM sodium butyrate for 4h)
    All lanes:HIST1H4A antibody at 1.2μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 12 KDa
    Observed band size: 12 KDa

  • Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429OA12forHU,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen : Peptide sequence around site of formyl-Lys(12) derived from human Histone H4
Raised in : Rabbit
Species Reactivity : Human, Rat
Tested Applications : ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
Relevance : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form : Liquid
Conjugate : Non-conjugated
Storage Buffer : Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification method : Antigen Affinity Purified
Isotype : IgG
Clonality : Polyclonal
Alias : Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, AND, HIST4H4
Species : Human
Research Area : Epigenetics and Nuclear Signaling
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