GAPDH Antibody

^{Rare Species}

Datasheet

Code	CSB-PA009232HA01DXU
Size	US$166
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Image	Western Blot Positive WB detected in Recombinant protein All lanes: GAPDH antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution predicted band size: 36 kDa observed band size: 36 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Cricetulus griseus GAPDH Polyclonal antibody

Uniprot No.

P17244

Target Names

GAPDH

Alternative Names

GAPDH antibody; GAPDGlyceraldehyde-3-phosphate dehydrogenase antibody; GAPDH antibody; EC 1.2.1.12 antibody; Peptidyl-cysteine S-nitrosylase GAPDH antibody; EC 2.6.99.- antibody

Raised in

Rabbit

Species Reactivity

Cricetulus griseus

Immunogen

Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA)

Immunogen Species

Cricetulus griseus

Conjugate

Non-conjugated

The GAPDH Antibody (Product code: CSB-PA009232HA01DXU) is Non-conjugated. For GAPDH Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA009232HB01DXU	GAPDH Antibody, HRP conjugated	ELISA
FITC	CSB-PA009232HC01DXU	GAPDH Antibody, FITC conjugated
Biotin	CSB-PA009232HD01DXU	GAPDH Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.

Subcellular Location

Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus.

Protein Families

Glyceraldehyde-3-phosphate dehydrogenase family

Database Links

KEGG: cge:100736557

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

How to Choose the Loading Control Antibodies? Glycolysis Pathway: The Essential Energy Source for Cells, Even in Low-Oxygen Conditions Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

HIF-1 signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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