GAPDH Antibody

Code CSB-PA009232HA01DXU
Size US$166
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  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: GAPDH antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    predicted band size: 36 kDa
    observed band size: 36 kDa

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Product Details

Full Product Name
Rabbit anti-Cricetulus griseus GAPDH Polyclonal antibody
Uniprot No.
Target Names
GAPDH
Alternative Names
GAPDH antibody; GAPDGlyceraldehyde-3-phosphate dehydrogenase antibody; GAPDH antibody; EC 1.2.1.12 antibody; Peptidyl-cysteine S-nitrosylase GAPDH antibody; EC 2.6.99.- antibody
Raised in
Rabbit
Species Reactivity
Cricetulus griseus
Immunogen
Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA)
Immunogen Species
Cricetulus griseus
Conjugate
Non-conjugated

The GAPDH Antibody (Product code: CSB-PA009232HA01DXU) is Non-conjugated. For GAPDH Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA009232HB01DXU GAPDH Antibody, HRP conjugated ELISA
FITC CSB-PA009232HC01DXU GAPDH Antibody, FITC conjugated
Biotin CSB-PA009232HD01DXU GAPDH Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Subcellular Location
Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus.
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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