GAS1 Antibody

Datasheet

Code	CSB-PA009264LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate All lanes: GAS1 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA009264LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA009264LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cells with CSB-PA009264LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GAS1 Polyclonal antibody

Uniprot No.

P54826

Target Names

GAS1

Alternative Names

GAS-1 antibody; Gas1 antibody; GAS1_HUMAN antibody; Growth arrest-specific protein 1 antibody; Growth-arrest-specific protein 1 precursor antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Growth arrest-specific protein 1 protein (248-318AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GAS1 Antibody (Product code: CSB-PA009264LA01HU) is Non-conjugated. For GAS1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA009264LB01HU	GAS1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA009264LC01HU	GAS1 Antibody, FITC conjugated
Biotin	CSB-PA009264LD01HU	GAS1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Gene References into Functions

Studies establish Gas1 as a negative regulator in colorectal cancer. PMID: 27401611
These results demonstrated that miR-184 and the GAS1/Akt pathway may be a potential therapeutic target for intervertebral disc degeneration. PMID: 26805687
WT1 modulates Akt/JNK signaling pathway mediated autophagy by controlling the expression of growth arrest-specific 1 (Gas1). PMID: 27453337
Downregulation of GAS1 is associated with clear cell renal cell carcinoma. PMID: 28184927
the synergistic effect of Gas1 inhibition and GDNF against glutamate-induced cell injury in human SH-SY5Y neuroblastoma cells, is reported. PMID: 26215053
solutions structure and biophysical characterization of the multifaceted signalling effector protein growth arrest specific-1 PMID: 25888394
This review summarizes the structure of Gas1 and molecular mechanism of action during embryonic development and cellular functions. PMID: 25429664
GAS1 is a novel endogenous inhibitor of glomerular mesangial cell proliferation. PMID: 23254899
Growth arrest-specific gene 1 is downregulated and inhibits tumor growth in gastric cancer. PMID: 22846196
GAS1 induced the activity of caspase-3 and resulting in apoptosis of the cells. PMID: 22311470
Interference of endoplasmic reticulum to Golgi transport increased immature amyloid beta protein precursor accumulation upon Gas1 expression in lipid raft fractions. PMID: 21971401
we demonstrate that sequence variants in GAS1 can impair its physical interaction with SHH, suggesting a decrease in the SHH downstream signaling cascade as a pathogenic mechanism of disease. PMID: 21842183
Gas1 is a novel APP-interacting protein involved in the control of APP maturation and processing. PMID: 21357679
Growth arrest-specific gene 1 contributes to predicting metastasis or recurrence in stage II and III colorectal cancer. PMID: 21111449
GAS1 could be considered a candidate locus for one of the types of human holoprosencephaly. PMID: 20583177
Gas1 is related to the GDNF alpha receptors and regulates Ret signaling PMID: 16551639
This review considers the possible involvement of GAS1 in genetic diseases, stem cell renewal, and cancer growth through its cooperative activity with Hedgehog (HH) growth factor and the HH receptor. PMID: 17726382
Gas1 is a molecule involved in cell arrest that can induce apoptosis when over-expressed in different cell lines. PMID: 18394855
Gas1 has all the expected properties of a melanoma tumor suppressor: suppression of metastasis in a spontaneous metastasis assay, promotion of apoptosis following dissemination of cells to secondary sites, and down-regulation in human melanoma metastasis. PMID: 18981472
Results present evidence demonstrating that Gas1 exerts its effects inhibiting cell growth and inducing apoptosis of glioma cells in the absence of Shh. PMID: 19460624
Results suggest that GAS1 might be a target to overcome multidrug resistance and provide a novel approach to identifying candidate genes that suppress chemoresistance of gastric cancers. PMID: 19638344
GAS1 is preferentially expressed by human bulge cells, compared to differentiated hair follicle keratinocytes PMID: 20050020

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Database Links

HGNC: 4165

OMIM: 139185

KEGG: hsa:2619

STRING: 9606.ENSP00000298743

UniGene: Hs.65029

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Hedgehog signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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