GHITM Antibody

Code CSB-PA872487LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Rat brain tissue
    All lanes: GHITM antibody at 2.7µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • IHC image of CSB-PA872487LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA872487LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA872487LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GHITM Polyclonal antibody
Uniprot No.
Target Names
GHITM
Alternative Names
GHITM; DERP2; MICS1; TMBIM5; My021; UNQ244/PRO281; Growth hormone-inducible transmembrane protein; Dermal papilla-derived protein 2; Mitochondrial morphology and cristae structure 1; Transmembrane BAX inhibitor motif-containing protein 5
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Growth hormone-inducible transmembrane protein (293-345AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GHITM Antibody (Product code: CSB-PA872487LA01HU) is Non-conjugated. For GHITM Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA872487LB01HU GHITM Antibody, HRP conjugated ELISA
FITC CSB-PA872487LC01HU GHITM Antibody, FITC conjugated
Biotin CSB-PA872487LD01HU GHITM Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c.
Gene References into Functions
  1. MICS1 individually functions in mitochondrial morphology and cytochrome c release. PMID: 18417609
  2. The tmbim5 may participate in cell death regulation by interacting with proteins of Bcl-2 family, promoting tumor metastasis, which is deduced from the evolutionary conservation of the membrane protein family containing multiple membrane spanning segments PMID: 18440869
Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Protein Families
BI1 family
Database Links

HGNC: 17281

KEGG: hsa:27069

STRING: 9606.ENSP00000361207

UniGene: Hs.352656

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