GPR146 Antibody

Code CSB-PA006863
Size US$167
Image
  • Western Blot analysis of LOVO cells using GPR146 Polyclonal Antibody
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Product Details

Uniprot No. Q96CH1
Target Names GPR146
Alternative Names G protein coupled receptor 146 antibody; G-protein coupled receptor PGR8 antibody; GP146_HUMAN antibody; GPR146 antibody; PGR8 antibody; Probable G-protein coupled receptor 146 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Synthesized peptide derived from the Internal region of Human GPR146.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Hypercholesterolemia risk-associated GPR146 is an orphan G-protein coupled receptor that regulates blood cholesterol levels in humans and mice. Fangfang Han,Cell Research,2020

Applications: Immunoblot analysis
Sample type: 293T cell
Review: Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction.
PMID: 32203133

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Target Data

Function Orphan receptor.
Gene References into Functions
  1. Knockdown of GPR146 blocked C-peptide-induced cFos expression in KATOIII cells. PMID: 23980258
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

HGNC: 21718

KEGG: hsa:115330

UniGene: Hs.585007

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