GPR161 Antibody

Code CSB-PA04114A0Rb
Size US$166
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Image
  • Western blot
    All lanes: GPR161 antibody at 2µg/ml + EC109 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/15000 dilution
    Predicted band size: 59, 46, 44, 50, 47, 61 kDa
    Observed band size: 30 kDa

  • Immunofluorescent analysis of Hela cells using CSB-PA04114A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GPR161 Polyclonal antibody
Uniprot No.
Target Names
GPR161
Alternative Names
GPR161 antibody; G-protein coupled receptor 161 antibody; G-protein coupled receptor RE2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human G-protein coupled receptor 161 protein (1-28AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GPR161 Antibody (Product code: CSB-PA04114A0Rb) is Non-conjugated. For GPR161 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA04114B0Rb GPR161 Antibody, HRP conjugated ELISA
FITC CSB-PA04114C0Rb GPR161 Antibody, FITC conjugated
Biotin CSB-PA04114D0Rb GPR161 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown.
Gene References into Functions
  1. Gpr161 is a critical factor in the basal suppression machinery of Shh signaling, neural tube morphogenesis and closure. (Review) PMID: 27731925
  2. Gpr161 is an A-kinase anchoring protein and the cAMP-sensing Gpr161:PKA complex acts as cilium-compartmentalized signalosome PMID: 27357676
  3. Smoothened determines beta-arrestin-mediated removal of the G protein-coupled receptor Gpr161 from the primary cilium. PMID: 27002170
  4. G-protein-coupled receptor GPR161 is overexpressed in breast cancer and is a promoter of cell proliferation and invasion. PMID: 24599592
  5. False positive non-synonymous polymorphisms of G-protein coupled receptor genes. PMID: 11959142

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Subcellular Location
Cell projection, cilium membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Database Links

HGNC: 23694

OMIM: 612250

KEGG: hsa:23432

STRING: 9606.ENSP00000271357

UniGene: Hs.271809

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