GPR75 Antibody

Code CSB-PA009829LA01HU
Size US$166
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  • Western blot
    All lanes: GPR75 antibody at 8µg/ml + HT29 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009829LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA009829LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunofluorescent analysis of A549 cells using CSB-PA009829LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GPR75 Polyclonal antibody
Uniprot No.
Target Names
GPR75
Alternative Names
G protein coupled receptor 75 antibody; GPR chr2 antibody; Gpr75 antibody; GPR75_HUMAN antibody; GPRchr2 antibody; OTTHUMP00000159608 antibody; Probable G protein coupled receptor 75 antibody; Probable G-protein coupled receptor 75 antibody; WI 31133 antibody; WI31133 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Probable G-protein coupled receptor 75 protein (372-540AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GPR75 Antibody (Product code: CSB-PA009829LA01HU) is Non-conjugated. For GPR75 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA009829LB01HU GPR75 Antibody, HRP conjugated ELISA
FITC CSB-PA009829LC01HU GPR75 Antibody, FITC conjugated
Biotin CSB-PA009829LD01HU GPR75 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Initially, a rabbit was inoculated with recombinant human GPR75 protein (372-540aa) to generate the GPR75 polyclonal antibody. The resulting antibodies were extracted from the rabbit serum and then purified using protein G affinity chromatography. The purity level of the GPR75 polyclonal antibody was measured to exceed 95%, indicating minimal impurities.

This GPR75 polyclonal antibody can recognize and bind to the GPR75 protein of human origin. Moreover, it has successfully validated its effectiveness in a range of experimental settings, including ELISA, WB, IHC, and IF, enabling the detection of levels, determination of presence and size, and analysis of the distribution of GPR75 protein.

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Target Background

Function
G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cel
Database Links

HGNC: 4526

OMIM: 606704

KEGG: hsa:10936

STRING: 9606.ENSP00000378195

UniGene: Hs.40763

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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