HLA-C Antibody

Code CSB-PA15099A0Rb
Size US$166
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Image
  • IHC image of CSB-PA15099A0Rb diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
  • Immunofluorescence staining of Hela cell with CSB-PA15099A0Rb at 1:80, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, A431 whole cell lysate
    All lanes: HLA-C antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 41 kDa
    Observed band size: 41 kDa
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Product Details

Uniprot No.
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human HLA-C protein (25-308AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The HLA-C Antibody (Product code: CSB-PA15099A0Rb) is Non-conjugated. For HLA-C Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA15099B0Rb HLA-C Antibody, HRP conjugated ELISA
FITC CSB-PA15099C0Rb HLA-C Antibody, FITC conjugated
Biotin CSB-PA15099D0Rb HLA-C Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
HLA-C polyclonal antibody CSB-PA15099A0Rb was produced in the rabbit immunized by using the Recombinant Human HLA-C protein (25-308AA) as the immunogen. The target protein HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain. Class I molecules play a critical role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. Diseases associated with HLA-C include Psoriasis 1 and Human Immunodeficiency Virus Type 1.
This Rabbit anti-Homo sapiens (Human) HLA-C Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the HLA-C proteins of human-origin and may be used to detect the endogenous levels of HLA-C protein.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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