Code | CSB-PA002942 |
Size | US$100 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:100-1:300 |
IF | 1:200-1:1000 |
ELISA | 1:20000 |
Applications : WB
Sample type: Human cells
Review: 293T cells were transfected with plasmids expressing Tau and DYRK1A and treated with the indicated doses of aristolactam BIII for 6 h. Total cell lysates were prepared and subjected to Western blotting with anti-Tau, antiphosphorylated Tau (at T212), and anti-DYRK1A antibodies. hnRNP A1 protein was analyzed as a loading control. Western blotting was performed twice, and representative data are presented.
By Anonymous
Applications : WB
Sample type: Mouse SMA fibroblasts
Review: SMA fibroblasts were treated with various concentrations of B. javanica extract, and the expression levels of representative splicing factors SRSF1 and hnRNP A1 were analyzed by western blotting. GAPDH proteins were also examined as a loading control.
By Anonymous
Applications : Western blot
Sample type: Cells
Sample dilution: 1:1000
Review: FLAG-tagged DYRK1A-WT, DYRK1A-E396ter, and DYRK1A-K188R plasmids were transiently co-expressed with Tau protein in 293T cells for 24h. Total cell extracts were harvested and subjected to western blotting with anti-FLAG, anti-phosphorylated-Tau (at T212, p-Tau), and anti-Tau antibodies. HnRNP A1 served as a loading control. Asterisks indicate nonspecifc proteins.
By Anonymous