HNRNPA1 Antibody

Code CSB-PA002942
Size US$167
Image
  • Western Blot analysis of COLO205 cells using hnRNP A1 Polyclonal Antibody
  • Western Blot analysis of Jurkat NIH-3T3 cells using hnRNP A1 Polyclonal Antibody
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Product Details

Uniprot No. P09651
Target Names HNRNPA1
Alternative Names HNRNPA 1 antibody; Helix destabilizing protein antibody; Helix-destabilizing protein antibody; Heterogeneous nuclear ribonucleoprotein A1 antibody; Heterogeneous nuclear ribonucleoprotein A1B protein antibody; Heterogeneous nuclear ribonucleoprotein B2 protein antibody; Heterogeneous nuclear ribonucleoprotein core protein A1 antibody; hnRNP A1 antibody; hnRNP core protein A1 antibody; HNRNPA1 antibody; HNRPA1 antibody; MGC102835 antibody; Nuclear ribonucleoprotein particle A1 protein antibody; ROA1_HUMAN antibody; Single strand DNA binding protein UP1 antibody; Single strand RNA binding protein antibody; Single-strand RNA-binding protein antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from the Internal region of Human hnRNP A1.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

A novel de novo heterozygous DYRK1A mutation causes complete loss of DYRK1A function and developmental delay. KS Lee,Scientific Reports,2020

Applications: Western blot
Antibody dilution factor: 1:1000
Sample type: Cells
Review: FLAG-tagged DYRK1A-WT, DYRK1A-E396ter, and DYRK1A-K188R plasmids were transiently co-expressed with Tau protein in 293T cells for 24h. Total cell extracts were harvested and subjected to western blotting with anti-FLAG, anti-phosphorylated-Tau (at T212, p-Tau), and anti-Tau antibodies. HnRNP A1 served as a loading control. Asterisks indicate nonspecifc proteins.
PMID: 32555303

Improvement of spinal muscular atrophy via correction of the SMN2 splicing defect by Brucea javanica (L.) Merr. extract and Bruceine D. Baek J, et al,Phytomedicine,2019

Sample type: SMA fibroblasts
Sample species: Mouse
Application: WB
Review: SMA fibroblasts were treated with various concentrations of B. javanica extract, and the expression levels of representative splicing factors SRSF1 and hnRNP A1 were analyzed by western blotting. GAPDH proteins were also examined as a loading control.
PMID: 31563042

Target Data

Function Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection
Gene References into Functions
  1. Moreover, SRSF10, hnRNP A1/A2 and Sam68 collaborate to drive the DNA damage-induced splicing response of several transcripts that produce components implicated in apoptosis, cell-cycle control and DNA repair. PMID: 29396485
  2. By using an integrative structural biology approach, we show that hnRNP A1 forms a 1:1 complex with pri-mir-18a where both RNA recognition motifs (RRMs) bind to cognate RNA sequence motifs in the terminal loop of pri-mir-18a. PMID: 29946118
  3. Data suggest that one way heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) regulates exon definition is to modulate the interaction of U2 small nuclear RNA auxiliary factor 2 (U2AF2) with decoy or bona fide 3' splice site (3'ss). PMID: 29650551
  4. Our results indicate that hnRNP A1 promotes cell survival and cell cycle progression following UVB radiation PMID: 29229447
  5. These findings indicated that hnRNPA1 is highly expressed in gastric cancer (GC)and promoted invasion by inducing Epithelialtomesenchymal transition (EMT) in GC cells. Thus, hnRNPA1 may be a potential therapeutic target for GC. PMID: 29484423
  6. A significant overlap between hnRNPA1 and DDX5 splicing targets and they share many closely linked binding sites. PMID: 30042133
  7. Results suggest that telomeric repeat-containing RNA (TERRA) regulates binding of heterogeneous nuclear ribonucleoprotein A1 to the telomere in a region surrounding the telomere, leading to a deeper understanding of the mechanism of TERRA function. PMID: 27958374
  8. mutation in HNRNPA1 located in the nuclear localization signal domain of hnRNPA1, enhances the recruitment of mutant hnRNPA1 into stress granules, indicating that an altered nuclear localization signal activity plays an essential role in amyotrophic lateral sclerosis pathogenesis. PMID: 29033165
  9. Rules of RNA specificity of hnRNP A1 revealed by global and quantitative analysis of its affinity distribution have been described. PMID: 28193894
  10. study showed that UVB induces alternative splicing of hdm2 by increasing the expression and the binding of hnRNP A1 to hdm2 full-length mRNA PMID: 26757361
  11. The authors show by NMR spectroscopy that both RNA recognition motifs of hnRNP A1 can bind simultaneously to a single bipartite motif of the human intronic splicing silencer ISS-N1, which controls survival of motor neuron exon 7 splicing. PMID: 28650318
  12. current knowledge of the involvement of hnRNPA1 in cancer, including its roles in regulating cell proliferation, invasiveness, metabolism, adaptation to stress and immortalization. PMID: 28791797
  13. Case Report: inflammatory myofibroblastic tumor (IMT) of the urinary bladder with a novel HNRNPA1-ALK fusion. PMID: 28504207
  14. EGF signaling upregulates an E3 ubiquitin (Ub) ligase adaptor, SPRY domain-containing SOCS box protein 1 (SPSB1), which recruits Elongin B/C-Cullin complexes to conjugate lysine 29-linked poly Ubiquitin chains onto hnRNP A1. PMID: 28084329
  15. hnRNP A1 directly binds to the 5' untranslated region of the RON mRNA and activates its translation through G-quadruplex RNA secondary structures PMID: 26930004
  16. We demonstrate that the wild-type sequence harbors an hnRNP A1 and hnRNP A2/B1-binding exonic splicing silencer (ESS) overlapping the 5'splice site (5'ss) that prevents pseudoexon inclusion.we demonstrate that splice switching oligonucleotide (SSO) mediated blocking of the pseudoexon 3'ss and 5'ss effectively restores normal GLA splicing PMID: 27595546
  17. The hnRNP A1 binding map can be used to identify potential targets for splice-switching oligonucleotides-based therapy. PMID: 27380775
  18. Biophysical titrations reveal that the 5'-AUAGC-3' bulge undergoes a conformational change to assemble a functional hnRNP A1-human enterovirus 71 RNA complex. PMID: 28625847
  19. PRMT5 regulates internal ribosome entry site-dependent translation via methylation of hnRNP A1. PMID: 28115626
  20. KRAS-E2F1-ILK-hnRNPA1 regulatory loop enables pancreatic cancer cells to promote oncogenic KRAS signaling and to interact with the tumor microenvironment to promote aggressive phenotypes. PMID: 26616862
  21. findings suggest that a new player, i.e., O-GlcNAcylation, regulates hnRNP A1 translocation and interaction with Trn1, possibly affecting its function PMID: 27913144
  22. The results of this study suggest that hnRNPA1 is the causative gene in the family with flail arm ALS. This further expanded the disease phenotype of hnRNPA1 mutations. PMID: 27694260
  23. Introduction of point mutations into the hnRNP A1-binding site or knockdown of hnRNP A1 expression promoted human papillomavirus 18 233;416 splicing and reduced E6 expression. PMID: 27489271
  24. Studies provides evidence that hnRNP A1 is a new autoantigen of Behcet's Disease and associated with deep vein thrombosis. PMID: 27211563
  25. these data suggest that the level of hnRNPA1 is strictly controlled to be within a certain range by the mRNA autoregulation in the physiological condition so that the cytotoxicity-causative alteration of hnRNPA1 expression does not take place. PMID: 28000042
  26. The CCAT1/miR-490/hnRNPA1 axis promotes gastric cancer migration, and it may have a possible diagnostic and therapeutic potential in gastric cancer. PMID: 26825578
  27. Data show that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) unfolds the HRAS protein i-motif. PMID: 26674223
  28. We demonstrate that an hnRNPA1 and CBX5 bi-directional core promoter fragment does not comprise intrinsic capacity for specific CBX5 down-regulation in metastatic cells PMID: 26791953
  29. The sST2-5'UTR contains an IRES element, which is activated by a MEK/ERK-dependent increase in cytoplasmic localization of hnRNP A1 in response to FGF2, enhancing the translation of sST2. PMID: 27168114
  30. hnRNP A1 is implicated in the free fatty acid-induced expression of SREBP-1a and of its target genes as well as in the lipid accumulation in hepatocytes. PMID: 26869449
  31. analysis of HIV-1 intron splicing silencer and its interactions with the UP1 domain of hnRNPA1 PMID: 26607354
  32. Our results indicate that the knockdown of hnRNP A1 has a specific function on the splicing of CD44 in breast cancer cells. PMID: 26151392
  33. HNRNPA1 might play an important role in lung adenocarcinoma cells. PMID: 26581508
  34. MiR-26a and mir-584 inhibit the binding of hnRNP A1-CDK6 mRNA and induce colorectal cancer cell apoptosis. PMID: 26494299
  35. NF-kappaB2/p52:c-Myc:hnRNPA1 Pathway Regulates Expression of Androgen Receptor Splice Variants and Enzalutamide Sensitivity in Prostate Cancer PMID: 26056150
  36. lowering the levels of hnRNP A1/A2 elicits defective transcription elongation on a fraction of P-TEFb-dependent genes, hence favoring the transcription of P-TEFb-independent genes PMID: 26011126
  37. In neuroblastoma cells, hnRNP A1 directly interacts with Drp1 mRNA at its 3'UTR region, and enhances translation potential without affecting mRNA stability. PMID: 26518267
  38. The disordered regions of key RNP granule components and the full-length granule protein hnRNPA1 can phase separate in vitro, producing dynamic liquid droplets. PMID: 26412307
  39. ALS-linked mutations in ubiquilin-2 or hnRNPA1 reduce interaction between ubiquilin-2 and hnRNPA1 PMID: 25616961
  40. The marked loss of hnRNPA1 in motor neurons with concomitant cytoplasmic aggregation of TDP-43 may represent a severe disturbance of mRNA processing, suggesting a key role in progressive neuronal death in amyotrophic lateral sclerosis . PMID: 25338872
  41. These findings suggested that hnRNP A1 plays key roles in the regulation of cell cycle progression and pathogenesis of Oral squamous cell carcinoma . PMID: 25752295
  42. Its mutation is proved to be a rare cause of amyotrophic lateral sclerosis, frontotemporal dementia, and inclusion body myopathy. in the nertherlands. PMID: 24612671
  43. miR-18a induces the apoptosis of colon cancer cells by directly binding to oncogenic hnRNP A1. PMID: 24166503
  44. Its mutation is rare in patients with frontotemporal lobar degeneration. PMID: 24119545
  45. Data reveal three distinct enthalpic contributions from the interactions of hnRNP Al (UP1) with the Na+ form of G-quadruplex DNA. PMID: 24831962
  46. findings show HNRNPA1 modulates expression of an alternatively spliced transcript of HMGCR by regulating splicing and altering RNA stability, resulting in reduced HMGCR enzyme activity and increased LDL-Cholesterol uptake; results suggest HNRNPA1 plays a role in the variation of cardiovascular disease risk and statin response PMID: 24001602
  47. HNRNPA1 is a novel transcriptional regulator of IL-6 expression, acting via the 5'-flanking sequence of the gene. PMID: 23985572
  48. The results describe a UP1 binding mechanism that is likely different from current models used to explain the alternative splicing properties of hnRNP A1. PMID: 24628426
  49. This functional hnRNP A1 deletion mutant is similar to a predicted hnRNP A1 isoform, which had not been previously experimentally characterized. PMID: 24530421
  50. The results of this study suggested that mutations in hnRNPA1, A2/B1, and A3 genes are a rare finding in amyotrophic lateral sclerosis. PMID: 23827524

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Involvement in disease Inclusion body myopathy with early-onset Paget disease with or without frontotemporal dementia 3 (IBMPFD3); Amyotrophic lateral sclerosis 20 (ALS20)
Subcellular Location Nucleus, Cytoplasm, Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs, Shuttles continuously between the nucleus and the cytoplasm along with mRNA, Component of ribonucleosomes (PubMed:17289661), SUBCELLULAR LOCATION: Cytoplasm
Database Links

HGNC: 5031

OMIM: 164017

KEGG: hsa:3178

STRING: 9606.ENSP00000341826

UniGene: Hs.546261

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