Product Details

Uniprot No.

Target Names

HNRNPA1

Alternative Names

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) (Helix-destabilizing protein) (Single-strand RNA-binding protein) (hnRNP core protein A1) [Cleaved into: Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed], HNRNPA1, HNRPA1

Species Reactivity

Human

Immunogen

A synthesized peptide from human HNRNPA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) serves as a critical regulator of mRNA metabolism, influencing pre-mRNA splicing, mRNA stability, and translation. This RNA-binding protein participates in telomere maintenance and has emerged as a significant factor in cancer biology, where its dysregulation contributes to altered splicing patterns that promote tumor progression. For researchers investigating RNA processing mechanisms, splicing regulation, or cancer-associated transcriptional changes, reliable detection of hnRNP A1 is essential.

This recombinant monoclonal antibody, generated against a synthetic peptide from human HNRNPA1, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your optimization efforts translate reliably across experiments and projects.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis detects hnRNP A1 in human cell lines including HT-29, K562, and Jurkat lysates, with an observed band at 34 kDa rather than the predicted 39 kDa—a difference likely attributable to proteolytic processing or post-translational modifications affecting electrophoretic mobility. Immunohistochemistry staining has been validated in paraffin-embedded human glioma and thyroid tissues, while immunofluorescence studies in HeLa cells reveal the expected nuclear localization pattern characteristic of this RNA-binding protein. Flow cytometry applications have been confirmed using Jurkat cells with clear separation from isotype controls.

Whether your research focuses on alternative splicing mechanisms, RNA-protein interactions, or the role of hnRNP A1 in disease pathology, this antibody provides a dependable tool for protein detection and localization studies across diverse experimental contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May bind to specific miRNA hairpins. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1.; (Microbial infection) May play a role in HCV RNA replication.; (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

Gene References into Functions

Moreover, SRSF10, hnRNP A1/A2 and Sam68 collaborate to drive the DNA damage-induced splicing response of several transcripts that produce components implicated in apoptosis, cell-cycle control and DNA repair. PMID: 29396485
By using an integrative structural biology approach, we show that hnRNP A1 forms a 1:1 complex with pri-mir-18a where both RNA recognition motifs (RRMs) bind to cognate RNA sequence motifs in the terminal loop of pri-mir-18a. PMID: 29946118
Data suggest that one way heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) regulates exon definition is to modulate the interaction of U2 small nuclear RNA auxiliary factor 2 (U2AF2) with decoy or bona fide 3' splice site (3'ss). PMID: 29650551
Our results indicate that hnRNP A1 promotes cell survival and cell cycle progression following UVB radiation PMID: 29229447
These findings indicated that hnRNPA1 is highly expressed in gastric cancer (GC)and promoted invasion by inducing Epithelialtomesenchymal transition (EMT) in GC cells. Thus, hnRNPA1 may be a potential therapeutic target for GC. PMID: 29484423
A significant overlap between hnRNPA1 and DDX5 splicing targets and they share many closely linked binding sites. PMID: 30042133
Results suggest that telomeric repeat-containing RNA (TERRA) regulates binding of heterogeneous nuclear ribonucleoprotein A1 to the telomere in a region surrounding the telomere, leading to a deeper understanding of the mechanism of TERRA function. PMID: 27958374
mutation in HNRNPA1 located in the nuclear localization signal domain of hnRNPA1, enhances the recruitment of mutant hnRNPA1 into stress granules, indicating that an altered nuclear localization signal activity plays an essential role in amyotrophic lateral sclerosis pathogenesis. PMID: 29033165
Rules of RNA specificity of hnRNP A1 revealed by global and quantitative analysis of its affinity distribution have been described. PMID: 28193894
study showed that UVB induces alternative splicing of hdm2 by increasing the expression and the binding of hnRNP A1 to hdm2 full-length mRNA PMID: 26757361
The authors show by NMR spectroscopy that both RNA recognition motifs of hnRNP A1 can bind simultaneously to a single bipartite motif of the human intronic splicing silencer ISS-N1, which controls survival of motor neuron exon 7 splicing. PMID: 28650318
current knowledge of the involvement of hnRNPA1 in cancer, including its roles in regulating cell proliferation, invasiveness, metabolism, adaptation to stress and immortalization. PMID: 28791797
Case Report: inflammatory myofibroblastic tumor (IMT) of the urinary bladder with a novel HNRNPA1-ALK fusion. PMID: 28504207
EGF signaling upregulates an E3 ubiquitin (Ub) ligase adaptor, SPRY domain-containing SOCS box protein 1 (SPSB1), which recruits Elongin B/C-Cullin complexes to conjugate lysine 29-linked poly Ubiquitin chains onto hnRNP A1. PMID: 28084329
hnRNP A1 directly binds to the 5' untranslated region of the RON mRNA and activates its translation through G-quadruplex RNA secondary structures PMID: 26930004
We demonstrate that the wild-type sequence harbors an hnRNP A1 and hnRNP A2/B1-binding exonic splicing silencer (ESS) overlapping the 5'splice site (5'ss) that prevents pseudoexon inclusion.we demonstrate that splice switching oligonucleotide (SSO) mediated blocking of the pseudoexon 3'ss and 5'ss effectively restores normal GLA splicing PMID: 27595546
The hnRNP A1 binding map can be used to identify potential targets for splice-switching oligonucleotides-based therapy. PMID: 27380775
Biophysical titrations reveal that the 5'-AUAGC-3' bulge undergoes a conformational change to assemble a functional hnRNP A1-human enterovirus 71 RNA complex. PMID: 28625847
PRMT5 regulates internal ribosome entry site-dependent translation via methylation of hnRNP A1. PMID: 28115626
KRAS-E2F1-ILK-hnRNPA1 regulatory loop enables pancreatic cancer cells to promote oncogenic KRAS signaling and to interact with the tumor microenvironment to promote aggressive phenotypes. PMID: 26616862
findings suggest that a new player, i.e., O-GlcNAcylation, regulates hnRNP A1 translocation and interaction with Trn1, possibly affecting its function PMID: 27913144
The results of this study suggest that hnRNPA1 is the causative gene in the family with flail arm ALS. This further expanded the disease phenotype of hnRNPA1 mutations. PMID: 27694260
Introduction of point mutations into the hnRNP A1-binding site or knockdown of hnRNP A1 expression promoted human papillomavirus 18 233;416 splicing and reduced E6 expression. PMID: 27489271
Studies provides evidence that hnRNP A1 is a new autoantigen of Behcet's Disease and associated with deep vein thrombosis. PMID: 27211563
these data suggest that the level of hnRNPA1 is strictly controlled to be within a certain range by the mRNA autoregulation in the physiological condition so that the cytotoxicity-causative alteration of hnRNPA1 expression does not take place. PMID: 28000042
The CCAT1/miR-490/hnRNPA1 axis promotes gastric cancer migration, and it may have a possible diagnostic and therapeutic potential in gastric cancer. PMID: 26825578
Data show that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) unfolds the HRAS protein i-motif. PMID: 26674223
We demonstrate that an hnRNPA1 and CBX5 bi-directional core promoter fragment does not comprise intrinsic capacity for specific CBX5 down-regulation in metastatic cells PMID: 26791953
The sST2-5'UTR contains an IRES element, which is activated by a MEK/ERK-dependent increase in cytoplasmic localization of hnRNP A1 in response to FGF2, enhancing the translation of sST2. PMID: 27168114
hnRNP A1 is implicated in the free fatty acid-induced expression of SREBP-1a and of its target genes as well as in the lipid accumulation in hepatocytes. PMID: 26869449
analysis of HIV-1 intron splicing silencer and its interactions with the UP1 domain of hnRNPA1 PMID: 26607354
Our results indicate that the knockdown of hnRNP A1 has a specific function on the splicing of CD44 in breast cancer cells. PMID: 26151392
HNRNPA1 might play an important role in lung adenocarcinoma cells. PMID: 26581508
MiR-26a and mir-584 inhibit the binding of hnRNP A1-CDK6 mRNA and induce colorectal cancer cell apoptosis. PMID: 26494299
NF-kappaB2/p52:c-Myc:hnRNPA1 Pathway Regulates Expression of Androgen Receptor Splice Variants and Enzalutamide Sensitivity in Prostate Cancer PMID: 26056150
lowering the levels of hnRNP A1/A2 elicits defective transcription elongation on a fraction of P-TEFb-dependent genes, hence favoring the transcription of P-TEFb-independent genes PMID: 26011126
In neuroblastoma cells, hnRNP A1 directly interacts with Drp1 mRNA at its 3'UTR region, and enhances translation potential without affecting mRNA stability. PMID: 26518267
The disordered regions of key RNP granule components and the full-length granule protein hnRNPA1 can phase separate in vitro, producing dynamic liquid droplets. PMID: 26412307
ALS-linked mutations in ubiquilin-2 or hnRNPA1 reduce interaction between ubiquilin-2 and hnRNPA1 PMID: 25616961
The marked loss of hnRNPA1 in motor neurons with concomitant cytoplasmic aggregation of TDP-43 may represent a severe disturbance of mRNA processing, suggesting a key role in progressive neuronal death in amyotrophic lateral sclerosis . PMID: 25338872
These findings suggested that hnRNP A1 plays key roles in the regulation of cell cycle progression and pathogenesis of Oral squamous cell carcinoma . PMID: 25752295
Its mutation is proved to be a rare cause of amyotrophic lateral sclerosis, frontotemporal dementia, and inclusion body myopathy. in the nertherlands. PMID: 24612671
miR-18a induces the apoptosis of colon cancer cells by directly binding to oncogenic hnRNP A1. PMID: 24166503
Its mutation is rare in patients with frontotemporal lobar degeneration. PMID: 24119545
Data reveal three distinct enthalpic contributions from the interactions of hnRNP Al (UP1) with the Na+ form of G-quadruplex DNA. PMID: 24831962
findings show HNRNPA1 modulates expression of an alternatively spliced transcript of HMGCR by regulating splicing and altering RNA stability, resulting in reduced HMGCR enzyme activity and increased LDL-Cholesterol uptake; results suggest HNRNPA1 plays a role in the variation of cardiovascular disease risk and statin response PMID: 24001602
HNRNPA1 is a novel transcriptional regulator of IL-6 expression, acting via the 5'-flanking sequence of the gene. PMID: 23985572
The results describe a UP1 binding mechanism that is likely different from current models used to explain the alternative splicing properties of hnRNP A1. PMID: 24628426
This functional hnRNP A1 deletion mutant is similar to a predicted hnRNP A1 isoform, which had not been previously experimentally characterized. PMID: 24530421
The results of this study suggested that mutations in hnRNPA1, A2/B1, and A3 genes are a rare finding in amyotrophic lateral sclerosis. PMID: 23827524

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Involvement in disease

Inclusion body myopathy with early-onset Paget disease with or without frontotemporal dementia 3 (IBMPFD3); Amyotrophic lateral sclerosis 20 (ALS20)

Subcellular Location

Nucleus. Cytoplasm.; Cytoplasm.

Database Links

HGNC: 5031

OMIM: 164017

KEGG: hsa:3178

STRING: 9606.ENSP00000341826

UniGene: Hs.546261

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Code	CSB-RA829343A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes:hnRNP A1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 39 kDa Observed band size: 34 kDa Exposure time：2min IHC image of CSB-RA829343A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA829343A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA829343A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA829343A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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HNRNPA1 Recombinant Monoclonal Antibody

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Target Background