HSPA1L Antibody

Datasheet

Code	CSB-PA010823HA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, L02 whole cell lysate, 293 whole cell lysate, Raji whole cell lysate All lanes: HSPA1L antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71 kDa Observed band size: 71 kDa IHC image of CSB-PA010823HA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA010823HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HSPA1L Polyclonal antibody

Uniprot No.

P34931

Target Names

HSPA1L

Alternative Names

Heat shock 70 kDa protein 1 Hom antibody; Heat shock 70 kDa protein 1 like antibody; Heat shock 70 kDa protein 1-Hom antibody; Heat shock 70 kDa protein 1-like antibody; Heat shock 70 kDa protein 1L antibody; Heat shock 70kD protein like 1 antibody; HS71L_HUMAN antibody; HSP70 1L antibody; HSP70 HOM antibody; HSP70-Hom antibody; HSPA1L antibody; hum70t antibody; Spermatid specific heat shock protein 70 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Heat shock 70 kDa protein 1-like protein (420-641AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HSPA1L Antibody (Product code: CSB-PA010823HA01HU) is Non-conjugated. For HSPA1L Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010823HB01HU	HSPA1L Antibody, HRP conjugated	ELISA
FITC	CSB-PA010823HC01HU	HSPA1L Antibody, FITC conjugated
Biotin	CSB-PA010823HD01HU	HSPA1L Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Positive regulator of PRKN translocation to damaged mitochondria.

Gene References into Functions

HSP70-HOM gene polymorphism associated with susceptibility to noise-induced hearing loss. PMID: 29072670
In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations. PMID: 28291257
A heterozygous de novo mutation (c.830C > T; p.Ser277Leu) in HSPA1L in ulcerative colitis patient. Five additional rare HSPA1L mutations (p.Gly77Ser, p.Leu172del, p.Thr267Ile, p.Ala268Thr, p.Glu558Asp) were found in six other patients. All six rare HSPA1L variant proteins showed decreased chaperone activity in vitro. Moreover, three variants demonstrated dominant negative effects on HSPA1L and HSPA1A protein activity. PMID: 28126021
Polymorphism of HSPA1L gene is associated with the development of esophageal Carcinoma. PMID: 26745065
may be a predictive biomarker for chronic graft-versus-host disease following allogeneic stem cell transplantation PMID: 25680846
HSPA1L (rs2227956) is associated with a decreased risk of idiopathic pulmonary fibrosis in a Mexican population. PMID: 26496868
Infertility in males with normal sperm parameters was not significantly associated with HSPA1L:c.1478C>T gene polymorphism. PMID: 26160076
HSP70-hom polymorphisms modify the association of diethylhexyl phthalates exposure with insulin resistance. PMID: 25044062
In HSPA1L T2437C polymorphisms, there was no significant differences in frequencies of the variant homozygous in patients compared to controls. PMID: 23666708
findings show polymorphisms of HSP70 genes are associated with the development of gastric cancer and duodenal ulcers in a population at high risk for gastric cancer in Costa Rica PMID: 24051039
studied the relationship between blood levels of HSP70 and HSP90 and genotypes of HSP70, GSTT1, and GSTM1 polymorphic variants in individuals chronically exposed to mercury PMID: 23330093
HSP70-hom gene polymorphism may have a role in graft-versus-host disease after hematopoietic stem cell transplantation PMID: 17060867
examined the relationship between the HSP70-hom polymorphism and the clinical characteristics of the malignancy at the time of diagnosis PMID: 17578680
A strong association was found between HSP-70/Hom rs2075800 G and uveitis in patients with sarcoidosis. PMID: 17591867
polymorphisms in HSP70-HOM genes associated with noise-induced hearing loss PMID: 18813331

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Protein Families

Heat shock protein 70 family

Tissue Specificity

Expressed in spermatids.

Database Links

HGNC: 5234

OMIM: 140559

KEGG: hsa:3305

STRING: 9606.ENSP00000364805

UniGene: Hs.690634

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Related pathways

Estrogen signaling pathway MAPK signaling pathway Protein processing in endoplasmic reticulum Antigen processing and presentation Endocytosis

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