IL10RB Antibody

Datasheet

Code	CSB-PA600091LA01HU
Size	US$299
Image	Western Blot Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue All lanes: IL10RB antibody at 2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 45 kDa IHC image of CSB-PA600091LA01HU diluted at 1:100 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA600091LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) IL10RB Polyclonal antibody

Uniprot No.

Q08334

Target Names

IL10RB

Alternative Names

Cd210 antibody; CDw210b antibody; CRF2 4 antibody; CRF2-4 antibody; CRFB4 antibody; Cytokine receptor class II antibody; Cytokine receptor class II CRF2 4 antibody; Cytokine receptor class-II member 4 antibody; Cytokine receptor family 2 member 4 antibody; Cytokine receptor family II member 4 antibody; D21S58 antibody; D21S66 antibody; I10R2_HUMAN antibody; IL-10 receptor subunit beta antibody; IL-10R subunit 2 antibody; IL-10R subunit beta antibody; IL-10R2 antibody; IL-10RB antibody; IL10R2 antibody; IL10RB antibody; Interleukin 10 receptor beta antibody; Interleukin-10 receptor subunit 2 antibody; Interleukin-10 receptor subunit beta antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Interleukin-10 receptor subunit beta protein (79-219AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The IL10RB Antibody (Product code: CSB-PA600091LA01HU) is Non-conjugated. For IL10RB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA600091LB01HU	IL10RB Antibody, HRP conjugated	ELISA
FITC	CSB-PA600091LC01HU	IL10RB Antibody, FITC conjugated
Biotin	CSB-PA600091LD01HU	IL10RB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function	Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
Gene References into Functions	IFN-lambda4 suppressed HIV infection of macrophages. This IFN-lambda4-mediated HIV inhibition was compromised by the antibodies against IFN-lambda receptor complex, IFN-lambdaR1/IL-10R2. PMID: 30247785 IL-10 and IL-10R gene polymorphisms may not contribute to the susceptibility to MM but may be associated with the severity and prognosis of MM. In particular, IL-10RB K47E polymorphism may contribute to the poor prognosis of MM patients treated with thalidomide and/or bortezomib. PMID: 27405747 systemic mRNA expression of IL10RB strongly differentiated children who failed to achieve asthma control with triamcinolone administration PMID: 27665382 IL10R2 Overexpression promotes IL22/STAT3 signaling in colorectal carcinogenesis. PMID: 26130064 High IL10RB expression is associated with diffuse large B-cell lymphoma. PMID: 25733167 PAPL, IL10RB and DEPDC5 polymorphisms have an impact on progression of hepatitis B virus-related liver disease. PMID: 25032264 Results show that the distribution of IL10RB and IL28RA genotypes among the Hepatitis C virus-infected and control groups did not differ significantly. PMID: 24144988 Mutations in the IL10RB gene is associated with ulcerative colitis. PMID: 24216686 IL-10RB rs2834167 (A/G) polymorphism may be a potential biomarker for susceptibility to systemic lupus erythematosus. PMID: 23749100 Case Report: pediatric ulcerative colitis patient compound heterozygote for the IL10RB E47K polymorphism, inherited from his father. PMID: 24379584 IL10RB polymorphisms are associated with Crohn's disease. PMID: 22550014 distinct receptor complex that is utilized by all three IFN-lambda proteins for signaling and is composed of two subunits, a receptor designated CRF2-12 (also designated as IFN-lambdaR1) and a second subunit, CRF2-4 (also known as IL-10R2) PMID: 12483210 sensitivity to recombinant interleukin-26(IL-26) of various cell lines strictly correlated with the expression of IL-20 receptor 1 and blocking antibodies against either IL-10 receptor 2 or IL-20 receptor 1 inhibited IL-26-dependent signal transduction PMID: 15178681 These findings suggested that signaling through IL-10R2 may play a causative role in dcSSc. PMID: 18588853 The IL-22R and IL-10R2 binding sites are juxtaposed on adjacent IL-22 surfaces contributed mostly by helices A, D, and F and loop AB. PMID: 18675824 gene polymorphism in combination with IL-10 gene polymorphism is assiociated with GvHD in HLA-identical donor-recipient pairs PMID: 19409109 Inhibition of S100A11 gene expression impairs the ability of keratinocytes to control vaccinia virus replication via downregulation of IFN-lambda receptor IL-10R2. PMID: 19577285 The association with the susceptibility to HBV infection was only observed for IL10RB K47E when we compared the individuals with persistent HBV infection through nonmaternal transmission to the controls with asymptomatic self-limited HBV infection. PMID: 19714778 Show More Hide All
Involvement in disease	Inflammatory bowel disease 25 (IBD25)
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Type II cytokine receptor family
Database Links	HGNC: 5965 OMIM: 123889 KEGG: hsa:3588 STRING: 9606.ENSP00000290200 UniGene: Hs.654593

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The Overview of Interferon Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Jak-STAT signaling pathway

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IL10RB Antibody

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