INSRR Antibody

Datasheet

Code	CSB-PA011755LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, MCF-7 whole cell lysatee, HepG2 whole cell lysate All lanes: INSRR antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 144 kDa Observed band size: 144 kDa IHC image of CSB-PA011755LA01HU diluted at 1:200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA011755LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INSRR Polyclonal antibody

Uniprot No.

P14616

Target Names

INSRR

Alternative Names

INSRR antibody; INSRR_HUMAN antibody; Insulin Receptor R antibody; Insulin receptor related receptor precursor antibody; Insulin receptor-related protein alpha chain antibody; Insulin receptor-related protein beta chain antibody; insulin receptor-related receptor antibody; IR R antibody; IR related receptor antibody; IR-related receptor antibody; IRR antibody; Sir r antibody; Sirr antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Insulin receptor-related protein (747-921AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The INSRR Antibody (Product code: CSB-PA011755LA01HU) is Non-conjugated. For INSRR Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011755LB01HU	INSRR Antibody, HRP conjugated	ELISA
FITC	CSB-PA011755LC01HU	INSRR Antibody, FITC conjugated
Biotin	CSB-PA011755LD01HU	INSRR Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Antigen Affinity purified & Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.

Gene References into Functions

the extensive glycosylation of FnIII-2/3 provides steric hindrance for the alkali-induced rearrangement of the IRR ectodomain. PMID: 29156593
IRR activation involves two separate centers of pH-dependent rearrangements that act synergistically to induce a major conformational change in the IRR molecule, resulting in internal kinase domains rapprochement and autophosphorylation. PMID: 25597417
IRR activation is not based on a single residue deprotonation in the IRR ectodomain but rather involves synergistic conformational changes at multiple points. PMID: 24121506
Insulin receptor-related receptor as an extracellular pH sensor involved in the regulation of acid-base balance. (Review) PMID: 23220417
Analysis of structural determinants of alkali sensor IRR positive cooperativity. PMID: 23824460
IRR mRNA expression was found in 25 (51%) neuroblastomas and correlated with stages 1, 2, 3, and 4S disease and with age. PMID: 14654552
Phosphotyrosine detection revealed a lack in the constitutive activation of the IRR described for analogous constructs of the two other members of the family PMID: 15629149
The IRR gene is expressed in the same ovarian compartment, IRR mRNA content increases strikingly in these cells in the afternoon of the first proestrus. PMID: 16195402

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily

Database Links

HGNC: 6093

OMIM: 147671

KEGG: hsa:3645

STRING: 9606.ENSP00000357178

UniGene: Hs.248138

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Related pathways

Regulation of actin cytoskeleton

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