INSRR Antibody

Datasheet

Code	CSB-PA011755LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, MCF-7 whole cell lysatee, HepG2 whole cell lysate All lanes: INSRR antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 144 kDa Observed band size: 144 kDa IHC image of CSB-PA011755LA01HU diluted at 1:200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA011755LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INSRR Polyclonal antibody

Uniprot No.

P14616

Target Names

INSRR

Alternative Names

INSRR antibody; INSRR_HUMAN antibody; Insulin Receptor R antibody; Insulin receptor related receptor precursor antibody; Insulin receptor-related protein alpha chain antibody; Insulin receptor-related protein beta chain antibody; insulin receptor-related receptor antibody; IR R antibody; IR related receptor antibody; IR-related receptor antibody; IRR antibody; Sir r antibody; Sirr antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Insulin receptor-related protein (747-921AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The INSRR Antibody (Product code: CSB-PA011755LA01HU) is Non-conjugated. For INSRR Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011755LB01HU	INSRR Antibody, HRP conjugated	ELISA
FITC	CSB-PA011755LC01HU	INSRR Antibody, FITC conjugated
Biotin	CSB-PA011755LD01HU	INSRR Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Antigen Affinity purified & Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.

Gene References into Functions

the extensive glycosylation of FnIII-2/3 provides steric hindrance for the alkali-induced rearrangement of the IRR ectodomain. PMID: 29156593
IRR activation involves two separate centers of pH-dependent rearrangements that act synergistically to induce a major conformational change in the IRR molecule, resulting in internal kinase domains rapprochement and autophosphorylation. PMID: 25597417
IRR activation is not based on a single residue deprotonation in the IRR ectodomain but rather involves synergistic conformational changes at multiple points. PMID: 24121506
Insulin receptor-related receptor as an extracellular pH sensor involved in the regulation of acid-base balance. (Review) PMID: 23220417
Analysis of structural determinants of alkali sensor IRR positive cooperativity. PMID: 23824460
IRR mRNA expression was found in 25 (51%) neuroblastomas and correlated with stages 1, 2, 3, and 4S disease and with age. PMID: 14654552
Phosphotyrosine detection revealed a lack in the constitutive activation of the IRR described for analogous constructs of the two other members of the family PMID: 15629149
The IRR gene is expressed in the same ovarian compartment, IRR mRNA content increases strikingly in these cells in the afternoon of the first proestrus. PMID: 16195402

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily

Database Links

HGNC: 6093

OMIM: 147671

KEGG: hsa:3645

STRING: 9606.ENSP00000357178

UniGene: Hs.248138

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Protein Tyrosine Kinases: Anti-tumor Targets to be Explored Urgently Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Regulation of actin cytoskeleton

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