INTS13 Antibody

Datasheet

Code	CSB-PA868331LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate All lanes: INTS13 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 69 kDa Observed band size: 81 kDa Immunoprecipitating INTS13 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA868331LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA868331LA01HU (6µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INTS13 Polyclonal antibody

Uniprot No.

Q9NVM9

Target Names

INTS13

Alternative Names

asun antibody; ASUN_HUMAN antibody; Cell cycle regulator Mat89Bb homolog antibody; Chromosome 12 open reading frame 11 antibody; FLJ10630 antibody; FLJ10637 antibody; Germ cell tumor 1 antibody; NET48 antibody; Protein asunder homolog antibody; Sarcoma antigen NY SAR 95 antibody; Sarcoma antigen NY-SAR-95 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Integrator complex subunit 13 protein (573-706AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The INTS13 Antibody (Product code: CSB-PA868331LA01HU) is Non-conjugated. For INTS13 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA868331LB01HU	INTS13 Antibody, HRP conjugated	ELISA
FITC	CSB-PA868331LC01HU	INTS13 Antibody, FITC conjugated
Biotin	CSB-PA868331LD01HU	INTS13 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Crucial regulator of the mitotic cell cycle and development. At prophase, required for dynein anchoring to the nuclear envelope important for proper centrosome-nucleus coupling. At G2/M phase, may be required for proper spindle formation and execution of cytokinesis. Probable component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

Gene References into Functions

ASUN is identified as a functional component of Integrator (INT), a multisubunit complex required for 3'-end processing of small nuclear RNAs. PMID: 23904267
Data suggest that ASUN promotes perinuclear enrichment of dynein at G2/M that facilitates BICD2- and CENP-F-mediated anchoring of dynein to nuclear pore complexes. PMID: 23097494

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Asunder family

Tissue Specificity

Widely expressed. Tends to be up-regulated in seminomas compared to normal testis.

Database Links

HGNC: 20174

OMIM: 615079

KEGG: hsa:55726

STRING: 9606.ENSP00000261191

UniGene: Hs.505077

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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