Name: KMT2E Antibody
Brand: CUSABIO
SKU: CSB-PA818269LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) KMT2E Polyclonal antibody

Uniprot No.

Q8IZD2

Target Names

KMT2E

Alternative Names

HDCMC04P antibody; Histone lysine N methyltransferase MLL5 antibody; Histone-lysine N-methyltransferase MLL5 antibody; KMT2E antibody; Lysine N methyltransferase 2E antibody; Lysine N-methyltransferase 2E antibody; MGC70452 antibody; Mll5 antibody; MLL5_HUMAN antibody; Myeloid/lymphoid or mixed lineage leukemia 5 (trithorax homolog; Drosophila) antibody; Myeloid/lymphoid or mixed lineage leukemia 5 antibody; Myeloid/lymphoid or mixed lineage leukemia protein 5 antibody; Myeloid/lymphoid or mixed-lineage leukemia protein 5 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Histone-lysine N-methyltransferase 2E protein (182-316AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The KMT2E Antibody (Product code: CSB-PA818269LA01HU) is Non-conjugated. For KMT2E Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA818269LB01HU	KMT2E Antibody, HRP conjugated	ELISA
FITC	CSB-PA818269LC01HU	KMT2E Antibody, FITC conjugated
Biotin	CSB-PA818269LD01HU	KMT2E Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription. Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry. Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition. During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells.; Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity.

Gene References into Functions

three dimensional structure of MLL5 SET domain unveils the structural basis for its lack of methyltransferase activity PMID: 27812132
MLL5 preserves spindle bipolarity through maintaining cytosolic PLK1 in a nonaggregated form. PMID: 27002166
MLL5 interacts with OGT and USP7 to form a stable ternary complex. Upregulation of MLL5 expression was correlated with increased expression of OGT and USP7 in human primary cervical adenocarcinomas. PMID: 26678539
Suggest a role for MLL5 and H3.3 in maintaining self-renewal hierarchies in adult glioblastomas. PMID: 26626085
O-GlcNAcylation of MLL5beta at T440 residue is critical for MLL5 recruitment to the HPV16/18-long control region through its interaction with AP-1. PMID: 25670814
Improved outcome is observed in decitabine-treated patients who express MLL5 at high levels. PMID: 24895338
KMT2E expression retained association with poor acute promyelocytic leukaemia remission rate and shorter survival while the association with disease-free survival was of marginal significance. PMID: 24796963
NMR solution structure of the MLL5 PHD domain PMID: 24130829
these results indicate that the suppression of MLL genes, especially MLL2 and MLL5, take part in modulating breast carcinogenesis. PMID: 23754336
MLL5 is a cellular ligand for the natural cytotoxicity receptor NKp44. PMID: 23958951
findings provide first insights into the molecular basis for the recruitment, exclusion, and regulation of MLL5 at chromatin PMID: 23798402
MLL5 can associate with HCF-1 and then be recruited to E2F1-responsive promoters to stimulate H3K4 trimethylation and transcriptional activation. PMID: 23629655
A new isoform, MLL5beta, truncated in exon 14, regulates E6 & E7 transcription in cervical carcinoma cells. Interaction of MLL5beta with the AP-1-binding site at the distal region of the HPV18 long control region activated E6/E7 transcription. PMID: 21908553
High MLL5 expression levels are associated with a favorable outcome and may improve risk and treatment stratification in acute myeloid leukemia PMID: 21205756
Phosphorylation of MLL5 may have an indispensable role in the mitotic progression in mixed lineage leukemia cells. PMID: 20439461
MLL5 forms intranuclear protein complexes that may play an important role in chromatin remodeling and cellular growth suppression. PMID: 14718661
These findings provide evidence that MLL5 might be an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. PMID: 18573682
MLL5 protein has been classified into a distinct subfamily with SETD5, because its SET domain and domain architecture show high homology with SETD5 rather than the members in MLL subfamily (i.e. MLL, MLL2, MLL3 and MLL4) PMID: 18231586
MLL5 is a homolog of Drosophila trithorax located within a segment of chromosome band 7q22 implicated in myeloid leukemia. PMID: 12101424

Hide All

Subcellular Location

Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle.; [Isoform 3]: Nucleus, nucleoplasm. Nucleus speckle.; [Isoform NKp44L]: Cytoplasm. Cell membrane; Peripheral membrane protein.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, TRX/MLL subfamily

Tissue Specificity

Widely expressed in both adult and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum. Isoform NKp44L: Not detected on circulating cells from healthy individuals, but

Database Links

HGNC: 18541

OMIM: 608444

KEGG: hsa:55904

STRING: 9606.ENSP00000257745

UniGene: Hs.592262

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

CAPTCHA verification *

Code	CSB-PA818269LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, NIH/3T3 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate All lanes: KMT2E antibody at 3.1μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 205, 196, 69, 99, 186, 181, 201, 132 kDa Observed band size: 80 kDa
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

KMT2E Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background