Krt18 Antibody

Datasheet
Code CSB-PA012519LA01MO
See More Details Free Antibody trial simple
Size US$299
Uniprot No. P05784
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, Mouse kidney tissue, Mouse liver tissue
    All lanes: Krt18 antibody at 5.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • IHC image of CSB-PA012519LA01MO diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA012519LA01MO diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Mouse Keratin, type I cytoskeletal 18 protein (200-423AA)
Raised in Rabbit
Species Reactivity Mouse, Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200
Relevance When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Keratin, type I cytoskeletal 18 (Cytokeratin endo B) (Keratin D) (Cytokeratin-18) (CK-18) (Keratin-18) (K18), Krt18, Kerd Krt1-18
Immunogen Species Mouse
Research Area Tags & Cell Markers
Target Names Krt18
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Subcellular Location Nucleus matrix, Nucleus, nucleolus, Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues.
Database Links

KEGG: mmu:16668

STRING: 10090.ENSMUSP00000023803

UniGene: Mm.22479

Pathway Estrogen signaling pathway

Related Products

Krt18 Antibodies

Krt18 Antibodies for Mus musculus (Mouse)

Krt18 Proteins

Krt18 Proteins for Mus musculus (Mouse)

Krt18 ELISA Kit

Krt18 ELISA Kit for Mus musculus (Mouse)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.