Product Details

Uniprot No.

Target Names

MBD4

Alternative Names

3 N(4) ethenocytosine glycosylase antibody; G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody; G/T mismatch glycosylase antibody; G/U mismatch glycosylase antibody; MBD 4 antibody; MBD4 antibody; MBD4_HUMAN antibody; MED 1 antibody; MED1 antibody; Methyl CpG binding domain protein 4 antibody; Methyl CpG binding endonuclease 1 antibody; Methyl CpG binding protein MBD4 antibody; Methyl-CpG-binding domain protein 4 antibody; Methyl-CpG-binding endonuclease 1 antibody; Methyl-CpG-binding protein MBD4 antibody; Mismatch specific DNA N glycosylase antibody; Mismatch-specific DNA N-glycosylase antibody; Putative methyl CpG binding protein antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Human MBD4

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Gene References into Functions

Results demonstrated that MBD4 was downregulated, which lead to the overexpression and promoter hypomethylation of CD70 in CD4+ T cells from patients from systemic lupus erythematosus (SLE). This study preliminarily revealed the role and mechanism of MBD4 in the pathogenesis of SLE. PMID: 29018507
these data suggest that MBD4 inactivation may contribute to tumorigenesis, acting as a modifier of mismatch repair-deficient cancer phenotype. PMID: 26503472
MBD4 rs3138373 A>G and rs2005618 T>C single nucleotide polymorphisms were not associated with esophageal squamous cell carcinoma (ESCC) risk. rs3138355 GG genotype was associated with a decreased risk of ESCC among male patients and the elderly. PMID: 25162968
Interaction between DNMT1 and MBD4 is involved in controlling gene expression and responding to oxidative stress. PMID: 24434851
ERCC4 rs1800124 and MBD4 rs10342 non-synonymous single nucleotide polymorphism variants were associated with DNA repair capacity. PMID: 24004570
the crystal structure of MBD4 bound to 5-hydroxymethylcytosine further demonstrates that MBDMBD4 is able to recognize a wide range of 5-methylcytosine modifications PMID: 23316048
MBD4 Glu346Lys polymorphism is associated with the risk of cervical cancer in a Chinese population. PMID: 23027038
Crystal structures of human MBD4(catalytic domain) reveal that MBD4 uses a base flipping mechanism to specifically recognize thymine and 5-hydroxymethyluracil. PMID: 22848106
specificity of MBD4 for acting at CpG sites depends largely on its methyl-CpG-binding domain, which binds preferably to G.T mispairs in a methylated CpG site PMID: 22560993
MBD4-8666 and MBD4-9229, but not MBD4-1057, gene polymorphisms are related to rheumatoid arthritis in Chinese patients in Taiwan. PMID: 20676650
MBD 4--a potential substrate for protein kinase X PMID: 21971312
the crystal structure of C-terminal glycosylase domain of human MBD4 was determined. PMID: 21820404
decreased expression in patients with primary immune thrombocytopenia PMID: 21377502
Data suggest that methyl-CpG binding domain 4 polymorphism may not be a stratification marker to predict the susceptibility to immune thrombocytopenic purpura, at least in the Chinese population. PMID: 20100009
MBD4 expression is associated with grade of malignancy in gliomas PMID: 11836615
results indicate the formation of a complex with the estradiol receptor PMID: 12220634
Frameshift mutations were found in 29% of gastric and 20% of colon MSI-H cancers, but not in any low-frequency microsatellite instability/microsatellite stable cancers. PMID: 12430186
MBD4 mutations were found in 15% MSI but not in MSS colrectal tumors. PMID: 12926109
MBD4 acts as a repressor protein binding to hypermethylated promoters of the p16(INK4a) and hMLH1 genes. PMID: 15899845
MBD4 Glu346Lys polymorphism could be used as a marker for genetic susceptibility to adenocarcinoma of the lung. PMID: 16803845
Mutations in MBD4 are unlikely to be implicated in HPS. PMID: 16831587
These results suggest that RFP is a mediator connecting several MBD proteins and allowing the formation of a more potent transcriptional repressor complex. PMID: 17049487
The overexpression of MBD4(tru) in Big Blue (lacI)-transfected, MSI human colorectal carcinoma cells doubled mutation frequency, indicating that the modest dominant negative effect on DNA repair can occur in living cells in short-term experiments. PMID: 17285135
MBD2 and MBD4 transcript overexpression and inverse correlations with DNA methylation indices indicate that both enzymes may really have a direct and active role on the genome-wide DNA hypomethylation observed in CD4+ T cells from SLE patients. PMID: 17360956
Truncation of MBD4 predisposes to reciprocal chromosomal translocations and alters the response to therapeutic agents in colon cancer cells. PMID: 18162445
Single nucleotide polymorphisms in MBD4 are associated with lung cancer. PMID: 18495292
MBD4 efficiently processed T/G mismatches within the nucleosome. PMID: 18519584
The methyl binding domain of MBD4/MED1 was found to specifically inhibit the activity of MBD4/MED1 as well as the glycosylase domain, when the G:IU mispairs were located in a methylated CpG context. PMID: 19395862
The Glu346Lys polymorphism and frameshift mutations of the Methyl-CpG Binding Domain 4 gene is associated with gastrointestinal cancer PMID: 19469655