MCM4 Antibody

Code CSB-PA010110
Size US$100
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Product Details

Uniprot No.
Target Names
MCM4
Alternative Names
19G antibody; AI325074 antibody; AU045576 antibody; CDC 21 antibody; CDC 54 antibody; CDC21 antibody; CDC21 homolog antibody; CDC21, S. pombe, homolog of antibody; CDC54 antibody; Cell division cycle 21, S. pombe, homolog of antibody; DNA replication licensing factor MCM 4 antibody; DNA replication licensing factor MCM4 antibody; hCdc 21 antibody; hCdc21 antibody; Homolog of S. pombe cell devision cycle 21 antibody; KIAA4003 antibody; mcdc21 antibody; MCM 4 antibody; MCM 4 minichromosome maintenance deficient 4 antibody; mcm4 antibody; MCM4 minichromosome maintenance deficient 4 antibody; MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody; MCM4_HUMAN antibody; Mcmd4 antibody; MGC33310 antibody; Minichromosome maintenance 4 antibody; Minichromosome maintenance complex component 4 antibody; Minichromosome maintenance deficient (S. cerevisiae) 4 antibody; Minichromosome maintenance deficient 4 antibody; Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody; Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody; mKIAA4003 antibody; NKCD antibody; NKGCD antibody; P1 CDC21 antibody; P1-CDC21 antibody; P1CDC21 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthesized peptide derived from Human MCM4 around the non-phosphorylation site of S54.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Gene References into Functions
  1. MCM4 and MCM7 expression is significantly correlated with Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma and precancerous lesions. PMID: 27476776
  2. This MCM4 mutation affected human MCM4/6/7 complex formation, since the complex containing the mutant MCM4 protein is unstable and the mutant MCM4 protein is tend to be degraded. PMID: 27794528
  3. We did not find any evidence of augmented response to a short-term (48 h) cisplatin treatment in these MCM4-deficient cells. However, MCM4-/HPV16+ SiHa cells cannot withstand a prolonged treatment (up to 5 days) of even a sublethal dosage of cisplatin PMID: 26188903
  4. Purified MCM4/6/7 complex containing the G364R MCM4 exhibited similar levels of single-stranded DNA binding and ATPase activities to the complex containing wild-type MCM4 PMID: 25661590
  5. Mutant p53 depletion profoundly influenced PARP1 localization and increased the level of PCNA and MCM4 proteins. PMID: 25733866
  6. Peroxisome proliferator-activated receptor gamma coactivator 1beta (PGC-1beta) protein attenuates vascular lesion formation by inhibition of chromatin loading of minichromosome maintenance complex in smooth muscle cells PMID: 23264620
  7. point mutation of MCM4 perturbs proper interaction with MCM6 to affect complex formation of MCM4/6/7 that is a core structure of MCM2-7 complex PMID: 22668557
  8. Mutations in MCM4/PRKDC represent a novel cause of DNA breakage and NK cell deficiency. PMID: 22499342
  9. Widdrol breaks DNA directly in HT29 cells, resulting in checkpoint activation via Chk2-p53-Cdc25A-p21-MCM4 pathway and finally cells go to G1-phase cell cycle arrest and apoptosis. PMID: 22160829
  10. partial MCM4 deficiency results in a genetic syndrome of growth retardation with adrenal insufficiency and selective NK deficiency PMID: 22354167
  11. MCM4 mutation may have a role in adrenal failure, short stature, and natural killer cell deficiency PMID: 22354170
  12. higher expression in non-small cell lung cancer PMID: 20884074
  13. MCM4 did not predict patient survival in this series of cutaneous melanomas. PMID: 20398247
  14. the phosphorylation of MCM4 in the checkpoint control inhibits DNA replication, which includes blockage of DNA fork progression, through inactivation of the MCM complex PMID: 12714602
  15. HU- or UV irradiation-stimulated phosphorylation of MCM4 at several CDK sites led to inhibition of MCM4 helicase activity, consistent with the notion that the phosphorylation of MCM4 is involved in regulation of DNA synthesis in the checkpoint control. PMID: 15037254
  16. These results suggest that phosphorylation of MCM4 has several distinct and site-specific roles in the function of MCM during the mammalian cell cycle. PMID: 16519687
  17. Expression of EBV-protein kinase caused phosphorylation of Thr-19 & Thr-110 on MCM4 PMID: 17005684
  18. MCM4 phosphorylation by Cdc7 kinase facilitates its interaction with Cdc45 on chromatin PMID: 17046832
  19. The positive rate of MCM4 was significantly higher in stage T3 than in stage T1 esophageal cancer. PMID: 17222376
  20. Data show that two fragments (148-441 and 442-676) from the central region of MCM2 were mainly responsible for the interaction between MCM2 and MCM4, and this was revealed by a pulldown analysis using MCM4 protein beads. PMID: 18190532
  21. Sequence alignment of archaeal MCMs and MCM4 from Homo sapiens and Saccharomyces cerevisiae shows that Aeropyrum pernix MCM shares 32% identity with human and yeast MCM4. PMID: 19053250

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Involvement in disease
Natural killer cell and glucocorticoid deficiency with DNA repair defect (NKGCD)
Subcellular Location
Nucleus. Chromosome.
Protein Families
MCM family
Database Links

HGNC: 6947

OMIM: 602638

KEGG: hsa:4173

STRING: 9606.ENSP00000262105

UniGene: Hs.460184

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