MKX Antibody

Datasheet

Code	CSB-PA818257LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate All lanes: MKX antibody at 6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa IHC image of CSB-PA818257LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA818257LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MKX Polyclonal antibody

Uniprot No.

Q8IYA7

Target Names

MKX

Alternative Names

9430023B20Rik antibody; C10orf48 antibody; Homeobox protein Mohawk antibody; IFRX antibody; Iroquois family related homeodomain protein antibody; iroquois homeobox protein like 1 antibody; IRXL1 antibody; MGC39616 antibody; MKX antibody; MKX_HUMAN antibody; mohawk homeobox antibody; OTTHUMP00000019374 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Homeobox protein Mohawk protein (157-305AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MKX Antibody (Product code: CSB-PA818257LA01HU) is Non-conjugated. For MKX Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA818257LB01HU	MKX Antibody, HRP conjugated	ELISA
FITC	CSB-PA818257LC01HU	MKX Antibody, FITC conjugated
Biotin	CSB-PA818257LD01HU	MKX Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May act as a morphogenetic regulator of cell adhesion.

Gene References into Functions

Data show that homeobox protein Mohawk (Mkx) is mainly expressed in the outer annulus fibrosus (OAF) of humans and mice. PMID: 27527664
these data shed light on previously unrecognized roles of Mkx in tendinopathy, tenogenesis, and tendon repair as well as in regulating the TGFbeta pathway. PMID: 25332192
Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo. PMID: 25312837
Reduced expression of MKX is a feature of degenerated anterior cruciate ligament in osteoarthritis-affected joints. PMID: 23686683
Observational study of gene-disease association. (HuGE Navigator) PMID: 20634891
In silico characterization of the C10orf48 sequence PMID: 16077953

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Subcellular Location

Nucleus.

Protein Families

TALE/IRO homeobox family

Database Links

HGNC: 23729

KEGG: hsa:283078

STRING: 9606.ENSP00000364946

UniGene: Hs.128193

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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