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Mono-methyl-H1F0 (K101) Antibody

Datasheet
Code CSB-PA010087OA101me1HU
Size US$319
Image

  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: H1F0 antibody at 1:50
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 21, 20 kDa
    Observed band size: 21 kDa

  • Immunofluorescence staining of HepG2 cells with CSB-PA010087OA101me1HU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H1F0 (CSB-PA010087OA101me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Immunocytochemistry analysis of CSB-PA010087OA101me1HU diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) H1F0 Polyclonal antibody
Uniprot No. P07305
Target Names H1F0
Alternative Names
H1 histone family member 0 antibody; H1(0) antibody; H10 antibody; H10_HUMAN antibody; h1f0 antibody; H1FV antibody; Histone H1'' antibody; Histone H1(0) antibody; Histone H1.0 antibody; Histone H10 antibody; Histone H5 antibody; MGC5241 antibody; N-terminally processed antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Mono-methyl-Lys (101) derived from Human Histone H1.0
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, ICC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:50-1:500
ICC 1:1-1:10
IF 1:1-1:10
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Gene References into Functions
  1. H1.X moves more rapidly than other linker histones in vivo Domain swapping between H1.0 and H1.X suggests that the globular domain (GD) and C-terminal domain (CTD) of H1.X independently contribute to the dynamic behavior of H1.X. PMID: 27528617
  2. this study shows that dynamic epigenetic states defined by the linker histone H1.0 determine which cells within a tumor can sustain the long-term cancer growth. PMID: 27708074
  3. The N-terminal domain contributes toward the differential chromatin binding affinity, whereas the C-terminal domain contributes toward distinct nucleosomal interface of isotypes H10 and H1c. PMID: 22334665
  4. nuclear transport of H1 histones requires a heterodimeric nuclear import receptor PMID: 12080050
  5. H1(0)histone may be an important factor in normal DC differentiation. Tumor-derived factors may inhibit DC differentiation by affecting H1(0) expression. PMID: 12149419

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Subcellular Location Nucleus. Chromosome. Note=The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
Protein Families Histone H1/H5 family
Database Links

HGNC: 4714

OMIM: 142708

KEGG: hsa:3005

STRING: 9606.ENSP00000344504

UniGene: Hs.745024
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