NCOA4 Antibody

Code CSB-PA015542ESR1HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015542ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA015542ESR1HU at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA015542ESR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) NCOA4 Polyclonal antibody
Uniprot No. Q13772
Target Names NCOA4
Alternative Names 70 kDa androgen receptor activator antibody; 70 kDa androgen receptor coactivator antibody; 70 kDa AR activator antibody; 70 kDa AR-activator antibody; Androgen receptor coactivator 70 kD antibody; Androgen receptor coactivator 70 kDa protein antibody; Androgen receptor-associated protein of 70 kDa antibody; ARA 70 antibody; ARA70 antibody; DKFZp762E1112 antibody; ELE 1 antibody; ELE1 antibody; ELE1/ret TK antibody; NCOA 4 antibody; NCoA-4 antibody; NCOA4 antibody; NCOA4_HUMAN antibody; Nuclear receptor coactivator 4 antibody; Papillary thyroid carcinoma 3 antibody; PTC 3 antibody; PTC3 antibody; RET activating gene ELE1 antibody; Ret activating protein ELE1 antibody; Ret-activating protein ELE1 antibody; RFG antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Nuclear receptor coactivator 4 protein (1-280AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
Gene References into Functions
  1. Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation. PMID: 28864529
  2. The H-ferritin could bind up to 24 NCOA4(383-522) fragments forming highly stable and insoluble complexes. The binding was partially inhibited only by Fe(II) among the various divalent metal ions analyzed. The iron-dependent, highly-specific formation of the remarkably stable H-ferritin-NCOA4 complex shown in this work may be important for the characterization of the mechanism of ferritinophagy. PMID: 28754384
  3. the ATG5-ATG7-NCOA4 autophagic pathway has a role in ferroptosis PMID: 27245739
  4. Ubiquitin-dependent NCOA4 turnover is promoted by excess iron and involves an iron-dependent interaction between NCOA4 and the HERC2 ubiquitin ligase. PMID: 26436293
  5. Studies indicate nuclear receptor coactivator 4 (NcoA4) as a coactivator for a variety of nuclear receptors. PMID: 22562579
  6. provide a new mechanism for selective autophagy of ferritin and reveal a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo PMID: 25327288
  7. RET/PTC3 gene rearrangements are the most prevalent form of rearrangements in papillary thyroid carcinomas of Chennai population. PMID: 24957039
  8. NCOA4 acts as a regulator of DNA replication origins that helps prevent inappropriate DNA synthesis and replication stress. PMID: 24910095
  9. identification of NCOA4 as a selective cargo receptor for autophagic turnover of ferritin (ferritinophagy), which is critical for iron homeostasis, and provides a resource for further dissection of autophagosomal cargo-receptor connectivity PMID: 24695223
  10. mechanism dissection shows that KLK2 may cooperate with the AR coregulator, ARA70, to enhance AR transactivation that may result in alteration of prostate cancer formation PMID: 24122203
  11. Data indicate that the increase in prostate cancer (PC) risk associated with rs10993994:C>T is likely mediated by the variant's effect of MSMB-encoded protein PSP94 expression; however, this effect does not extend to NCOA4 in the data. PMID: 22887727
  12. Computational network analysis reveals that the MSMB gene is functionally connected to NCOA4 and the androgen receptor signaling pathway. The data provide an example of how GWAS-associated variants may have multiple genetic and epigenetic effects PMID: 22661295
  13. Suppression of MSMB expression or NCOA4 overexpression promotes anchorage-independent growth of prostate epithelial cells. PMID: 21085629
  14. ARA70alpha functions as a tumor suppressor gene PMID: 20167864
  15. Screening for BRAF, RET, KRAS, NRAS, and HRAS mutations, as well as RET-PTC1 and RET-PTC3 rearrangements, was performed on cases of Hashimoto thyroiditis with a dominant nodule PMID: 20012784
  16. Potent mitogenicity of the RET/PTC3 oncogene correlates with its prevalence in tall-cell variant of papillary thyroid carcinoma. PMID: 11786418
  17. Domain interactions between coregulator ARA(70) and the androgen receptor (AR); structure activity relationship PMID: 11818501
  18. ARA70 has a role in enhancing the androgen receptor during testicular development PMID: 12068007
  19. role in reducing agonist activity of antiandrogens PMID: 12649293
  20. causes increases in nuclear NF-kappaB activity and secretion of MCP-1 and GM-CSF; studies implicate oncogene-induced cytokine-signaling pathways in a new mechanism linking inflammation with cancer PMID: 12881713
  21. ARA70 was identifed as a coactivator of PPARalpha in the prostate cancer cell line DU145. In the adrenal cell line Y1, ARA70 behaved as a repressor of PPARalpha while still able to coactivate PPARgamma. PMID: 12897377
  22. The refolding and one-step purification of ARA70 from inclusion bodies over-expressed in Escherichia coli is reported. PMID: 15642480
  23. ARA70 is a coactivator for estrogen receptor alpha (ERalpha) and may represent a functional link between ERalpha/androgen receptor (AR) modulating their cross-talk in models of estrogen signaling in MCF-7 and HeLa cells PMID: 15772083
  24. Together, ARA54 and ARA70 modulate vitamin D receptor transactivation, and the competition for ARA70 mediates the suppressive affect of androgen-androgen receptor on vitamin D receptor transactivation. PMID: 15805579
  25. a functional interaction between NcoA4 and AhR that may alter AhR activity to affect disease development and progression is demonstrated PMID: 16762319
  26. Expression of androgen receptor co-regulators in the testes of men with azoospermia. PMID: 17919607
  27. in prostate cancer cell lines, ARA70beta localized to the cytosol, whereas ARA70alpha was found in the nucleus, supporting the notion of their dissimilar functions PMID: 18091327
  28. ARA70 beta is a regulator of tumor cell growth and metastasis by affecting gene expression PMID: 18156210

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Involvement in disease A chromosomal aberration involving NCOA4 is found in papillary thyroid carcinomas (PTCs). Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene.
Tissue Specificity Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
Database Links

HGNC: 7671

OMIM: 601984

KEGG: hsa:8031

STRING: 9606.ENSP00000395465

UniGene: Hs.643658

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