NDUFB6 Antibody

Code CSB-PA015653LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue, Mouse thymus tissue
    All lanes: NDUFB6 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 16, 14 kDa
    Observed band size: 16, 14 kDa

  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA015653LA01HU at dilution of 1:100

  • Immunoprecipitating NDUFB6 in HEK293 whole cell lysate
    Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA015653LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA015653LA01HU (4µg) + HEK293 whole cell lysate (500µg)
    Lane 3: HEK293 whole cell lysate (20µg)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NDUFB6 Polyclonal antibody
Uniprot No.
Target Names
NDUFB6
Alternative Names
NDUFB6; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; Complex I-B17; CI-B17; NADH-ubiquinone oxidoreductase B17 subunit
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 protein (2-67AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NDUFB6 Antibody (Product code: CSB-PA015653LA01HU) is Non-conjugated. For NDUFB6 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA015653LB01HU NDUFB6 Antibody, HRP conjugated ELISA
FITC CSB-PA015653LC01HU NDUFB6 Antibody, FITC conjugated
Biotin CSB-PA015653LD01HU NDUFB6 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Gene References into Functions
  1. we propose that NDUFB6 is a possible tumor suppressor of metastatic ccRCCs. PMID: 25315157
  2. NDUFS6 has a Zn-binding site and is essential for biogenesis of mitochondrial complex I PMID: 25902503
  3. NDUFB6 subunit is required for complex I activity. PMID: 21964293
  4. Results demonstrate that genetic, epigenetic, and nongenetic factors associate with NDUFB6 expression in human muscle and suggest that genetic and epigenetic factors may interact to increase age-dependent susceptibility to insulin resistance. PMID: 17948130
  5. Polymorphism in the NDUFB6 gene from respiratory chain complex I related to ATP synthesis and insulin sensitivity response to exercise training found in relatives of humans with type 2 diabetes. PMID: 19265027

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Subcellular Location
Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
Protein Families
Complex I NDUFB6 subunit family
Database Links

HGNC: 7701

OMIM: 603322

KEGG: hsa:4712

STRING: 9606.ENSP00000369176

UniGene: Hs.493668

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