NPRL3 Antibody

Datasheet

Code	CSB-PA619643LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SY5Y whole cell lysate(30µg), U87 whole cell lysate(30µg), K562 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg),Hela whole cell lysate(30µg),PC-3 whole cell lysate(30µg),JK whole cell lysate(30µg) All lanes: NPRL3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 10s IHC image of CSB-PA619643LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA619643LA01HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA619643LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NPRL3 Polyclonal antibody

Uniprot No.

Q12980

Target Names

NPRL3

Alternative Names

otein antibody; Alpha-globin regulatory element-containing gene protein antibody; C16ORF35 antibody; CGTHBA antibody; Conserved gene telomeric to alpha globin cluster antibody; HS 40 antibody; MARE antibody; Nitrogen permease regulator 3-like protein antibody; Nitrogen permease regulator-like 3 (S. cerevisiae) antibody; NPR3 antibody; NPRL3 antibody; NPRL3_HUMAN antibody; Protein CGTHBA antibody; RMD11 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from human NPRL3 AA range: 465-510

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.

Gene References into Functions

This study demonstrated that mutation of NPRL3 in familial focal epilepsies and focal cortical dysplasia. PMID: 27173016
NPRL3 mutations are significant cause of focal epilepsy. PMID: 26505888
Mutations in NPRL3 are a novel cause of familial cortical dysplasia. PMID: 26285051
NPRL3 is a candidate gene for harbouring mutations in individuals with developmental abnormalities of the cardiovascular system. PMID: 22538705
NPRL3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
C16orf35 can modulate differentially the specific activities of selected p73 isoforms. PMID: 19666006

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Involvement in disease

Epilepsy, familial focal, with variable foci 3 (FFEVF3)

Subcellular Location

Lysosome membrane.

Protein Families

NPR3 family

Tissue Specificity

Widely expressed. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes.

Database Links

HGNC: 14124

OMIM: 600928

KEGG: hsa:8131

STRING: 9606.ENSP00000382834

UniGene: Hs.19699

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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