NPRL3 Antibody

Datasheet

Code	CSB-PA619643LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U87 whole cell lysate All lanes: NPRL3 antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa IHC image of CSB-PA619643LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NPRL3 Polyclonal antibody

Uniprot No.

Q12980

Target Names

NPRL3

Alternative Names

otein antibody; Alpha-globin regulatory element-containing gene protein antibody; C16ORF35 antibody; CGTHBA antibody; Conserved gene telomeric to alpha globin cluster antibody; HS 40 antibody; MARE antibody; Nitrogen permease regulator 3-like protein antibody; Nitrogen permease regulator-like 3 (S. cerevisiae) antibody; NPR3 antibody; NPRL3 antibody; NPRL3_HUMAN antibody; Protein CGTHBA antibody; RMD11 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human GATOR complex protein NPRL3 protein (349-482AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NPRL3 Antibody (Product code: CSB-PA619643LA01HU) is Non-conjugated. For NPRL3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA619643LB01HU	NPRL3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA619643LC01HU	NPRL3 Antibody, FITC conjugated
Biotin	CSB-PA619643LD01HU	NPRL3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.

Gene References into Functions

This study demonstrated that mutation of NPRL3 in familial focal epilepsies and focal cortical dysplasia. PMID: 27173016
NPRL3 mutations are significant cause of focal epilepsy. PMID: 26505888
Mutations in NPRL3 are a novel cause of familial cortical dysplasia. PMID: 26285051
NPRL3 is a candidate gene for harbouring mutations in individuals with developmental abnormalities of the cardiovascular system. PMID: 22538705
NPRL3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
C16orf35 can modulate differentially the specific activities of selected p73 isoforms. PMID: 19666006

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Involvement in disease

Epilepsy, familial focal, with variable foci 3 (FFEVF3)

Subcellular Location

Lysosome membrane.

Protein Families

NPR3 family

Tissue Specificity

Widely expressed. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes.

Database Links

HGNC: 14124

OMIM: 600928

KEGG: hsa:8131

STRING: 9606.ENSP00000382834

UniGene: Hs.19699

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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