NSF Antibody

Datasheet

Code	CSB-PA020159
Size	US$100
	Order now
Image	Western Blot analysis of CoLo cells using NSF Polyclonal Antibody
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P46459

Target Names

NSF

Alternative Names

N ethylmaleimide sensitive factor antibody; N ethylmaleimide sensitive factor like protein antibody; N ethylmaleimide sensitive fusion protein antibody; N-ethylmaleimide-sensitive fusion protein antibody; NEM sensitive fusion protein antibody; NEM-sensitive fusion protein antibody; NSF antibody; NSF_HUMAN antibody; SKD 2 antibody; SKD2 antibody; Vesicle fusing ATPase antibody; Vesicle-fusing ATPase antibody; Vesicular fusion protein NSF antibody; Vesicular-fusion protein NSF antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the Internal region of Human NSF.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:40000

Protocols

Western Blotting(WB) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.

Gene References into Functions

A large copy number variant (CNV) that encompasses part of the NSF gene contributes to cocaine dependence. PMID: 27498889
NSF deficiency in HeLa cells barely affected cell viability, anterograde trafficking of vesicular stomatitis virus glycoprotein G and transferrin endocytosis. PMID: 27995606
a processive helicase-like mechanism for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule. PMID: 23775070
Single and multiple-marker analyses revealed a strong association between cocaine dependence and the NSF gene. PMID: 21426264
Knockdown of TRX1 increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis, and suppresses leukocyte PMID: 21324905
NSF independent fusion of Salmonella-containing late phagosomes with early endosomes. PMID: 20176016
analysis of human NSF possible binding mode to GABARAP and GATE-16 PMID: 19533740
binding of NSF to GluR2-containing AMPARs in stabilizing these receptors in the synaptic membrane and impeding their regulated endocytosis. PMID: 12011465
role of interaction with GluR2 in regulation of AMPA receptors by brain-derived neurotrophic factor PMID: 12130635
Nitric oxide regulates exocytosis by S-nitrosylation of NSF. PMID: 14567912
Expressing a biochemically defined NSF mutant that lacks ATPase activity results in a dramatic inhibition of stimulated mast cell exocytosis. PMID: 14607937
NSF dissociates the VAMP-2.SNAP-25.syntaxin 13 complex, but has no effect on the Hrs-containing complex. PMID: 14769786
Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein PMID: 16461345
dual regulation on hbeta2AR trafficking and signaling by NSF through direct binding to cargo receptor and its ATPase activity. PMID: 17510209
Data show that NSF is an inclusion body component in neuronal intranuclear inclusion disease identified by anti-SUMO-1-immunocapture. PMID: 18836734
Data show that phosphorylation of N-ethylmaleimide-sensitive factor on tyrosine residues prevents its SNARE complex dissociation activity and establish a role for PTP1B in the modulation of the membrane fusion machinery. PMID: 19208619

Hide All

Subcellular Location

Cytoplasm.

Protein Families

AAA ATPase family

Database Links

HGNC: 8016

OMIM: 601633

KEGG: hsa:4905

STRING: 9606.ENSP00000381293

UniGene: Hs.431279

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Protein Acetylation: An Important Post-translational Modification in Eukaryotic Cells Oncogenes and Cancer Neuronal Markers and Neurodegenerative Diseases Claudin 18.2, is a 'Dark Horse' in Anti-tumour Therapy? What Are Histones: Unraveling Their Critical Roles in Disease Pathogenesis Melanoma, the Most Serious Type of Skin Cancer Non-Hodgkin's Lymphoma, One of Common Heterogeneous Malignant Tumors Information You Must Know about KCNN4 STAT3: a Key Transcriptional Regulator in Cancer MUC16 (CA125): a Promising Predictive Marker and Therapeutic Target in Cancer Two Types of Macrophages: M1 and M2 Macrophages EGFR and Its Application in Cancer Therapy An Overview of Cell Death: Necrosis and Apoptosis The Overview of One Category of Important Cell Receptors: Receptor Tyrosine Kinases Lysosomal Enzymes and Lysosomal Storage Disorders (LSDs) Cellular Senescence and Its Markers NKG2D, a Powerful Molecule Involved In CAR-T And Car-NK Cell Therapy Does LIF Only Play a Role in Leukaemia? How to Choose? Polyclonal, Monoclonal, or Recombinant Antibody? DNA Damage and Repair TFPI: a Natural Anticoagulant Protein, Future Therapies for Hemophilia Chemokine Receptor CCR4: the Key Role of CCR4+Treg Cells in Cancer Immunotherapy Proteolysis Targeting Chimera (PROTAC): A CLDN9: Another Key Regulator for Tumors or Hearing Loss from the CLDN Family! DDR1: a Novel Member of the RTKs Family, a Potential Target for Tumor Therapy! How to Validate An Antibody？ With the High Potential in Cancer Immunotherapy, CD40 Is Triggering Attention CD14, A Membrane Receptor for Bacterial Lipopolysaccharides Surface Markers That Help You Identify Lymphocytes How Do You Get Exosomes? Cell Differentiation Determines the Fate of Cells CAR-T Cell Therapy- An Useful Treatment To Cancer Exploring Biological Landscape of Cervical Cancer: A Comprehensive Overview for Researchers CLDN4: A Potential Prognostic Indicator and Therapeutic Target in Tumors！ TFR1 (TFRC): a Stimulator of Fe Transport Affects Anemia, Neurodegenerative Disorders and Cancers! SLC7A11: a New Potential Therapeutic Target via Induced Ferroptosis in Tumors! Protein Phosphorylation: The Dynamic Language of Cellular Regulation Tuberculosis--A Resurgent Differentiation of Th17 Cells A Review of WFDC8 Antibody Tag Antibodies, Your Good Partner in Protein Research ULBP1: a Potent Member of NKG2D Ligands in CAR-T Therapy for Multiple Hematological and Solid Tumors! How to Choose the Loading Control Antibodies? GFRAL: A Promising Metabolic Target for Improving Obesity and Anorexia/Cachexia Microglial Markers: Insights into Microglial Activation and Function Homeobox Transcription Factor- NANOG How to Inquire and Utilize the Signal Pathway on KEGG Costimulatory/Coinhibitory: Is B7-H3 An Enemy or A Friend? The Overview of Fc Receptors- Classification, Related Signaling Pathways and Application The Overview of Recombinant Antibody Glial Fibrillary Acidic Protein - Astrocyte Specific Marker MYL9: a New Member of Myosin Motors, a Key Driver of Cancer Cell Dynamic Regulation? New Mechanism, NRP1 Enhances the Ability of SARS-CoV-2 Infection Surface Markers of Mesenchymal Stem Cells Oct4 -- The Gatekeeper of Pluripotency of Embryonic Stem Cells CEACAM8: a Member of CEA Family, Emerging as a Vital Player in Gynaecological or Gastrointestinal Cancer Research! Stem Cell-What a Miraculous Resource in Human Body Growth Hormone - Not Only Promotes Height Increase The Application of IHC in Pathological Diagnosis LPS/TLR4 Signal Transduction Pathway Toll-like Receptors--The Eye of Innate Immunity A Comprehensive Exploration into Amyotrophic Lateral Sclerosis ACVRL1 (Alk1): a Member of the TGF-β Receptor Family, a Novel Target for Anti-Angiogenic Drug Research! The Meaning of Irregular Antibody Screening A "trembling" Parkinson's Disease Focus on Emerging Therapeutic Targets BCMA What Are Immune Checkpoint Pathways? Hematopoietic Stem Cell Surface Marker CD20 Monoclonal Antibodies Drugs: There is an Explosion of New mAbs Against CD20! Tumor Necrosis Factor Receptor Superfamily and Apoptosis The Overview of Coagulation The Power and Promise of Stable Cell Lines Understanding of Serum Albumin VSIG4: a Prominent B7 Family Costimulatory Molecule or Complement Receptor in Cancers and Inflammatory Diseases! SLC39A6/ZIP6: a Key Member of Zinc Transporters, a Novel Targeting Antigen of ADC! TMEFF2: a Tumor-Specific Methylated Biomarker, a Novel Target in Multiple Cancers Protein Kinases Overview: Definition, Types, Function, Activation Mechanism, etc. The Overview of Hippo Signaling Pathway Exploring Neurodegeneration: A Comprehensive Guide for Researchers CD44- A Powerful Bridge Between Cells Tools You Need For Fundamental Research How do Th1 and Th2 Cells Differentiate? CD22- What A Powerful Co-receptor of B Cell Receptor Protein Tyrosine Kinases: Anti-tumor Targets to be Explored Urgently Epigenetic Histone Modifications Pathway Unraveling the Dynamic World of Histone Modifications: Implications in Gene Regulation and Disease Pathogenesis Identify Treg Cells. You Can Do This. About Thyroid Tumor, This Information You Must Know! What Essential Biological Knowledge is Needed for Hepatocellular Carcinoma Research? What Are the Effects of Abnormalities in the ALK Gene CD146, A Powerful Tumor Marker Introduction of Hepatocellular Carcinoma Related Signaling Pathways Mesothelin: A Rising Star of Cancer Immunotherapy CD70 (CD27L/TNFSF7): a TNF Receptor Member, a “Star” Target for Clinical Research in Systemic Lupus Erythematosus (SLE), Arthritis, and Tumors! Epitope Tags Overview CRTAM: Class I Restricted T cell-associated Molecule, an Intriguing New Member of the Ig-SF Family! Does TNF Superfamily Inhibit Cancer or Promote Its Development? TMPRSS2, an Activator for SARS-CoV-2 Invasion TROP2, An Important Target for Cancer Research Surface Markers of Natural Killer Cells Mucin-17/MUC17: An Unexpected Target for Tumor Immune Therapy, Notably Gastric Cancer! Applications of recombinant proteins How to choose a suitable expression vector? How to express a protein with bioactivity? IL-17A: A Major Cytokine of the IL-17 Family, a Key Inflammation Target in Skin Diseases, or Inflammation-Associated Cancers! CUSABIO's Five Expression Systems Serotransferrin/TF: a Major Transferrin in Plasma, a Novel TF-Modified Nanoparticle for Tumor-Targeting Research! Myosin Regulatory Light Chains (MYL12A and MYL12B): the New Functional Ligands for CD69, Potential Targets for Cardiac Disease or Tumors Research? Do You Really Know Neurons and Glia Cells in Nerve System? Apoptosis mediated by mitochondria Apoptosis mediated by death receptor Get An Overview of His-Tag CD69: a Classical Early Marker of Lymphocyte Activation, a Pivotal Regulator of Hematologic Diseases and Autoimmune Disorders! Cadherin-17 (CDH17): A Viable Target in Bispecific Antibodies or CAR-T Therapy to Fight Many Gastrointestinal Cancers! Itaconate is a negative regulator of inflammation The Overview of Growth Factor The Overview of Interferon A Group of Promising Proteins - Cell Cycle Markers The Overview of Organelles The Overview of Autophagy Production of Recombinant Protein The Overview of Phage Display：Protocol, Classification, Application and FAQs TTR: an Important Protein of Amyloidosis, Novel Therapies are Upcoming for Rare Diseases ATTR-CM and ATTR-PN! The Overview of Breast Cancer: Related Signaling Pathways, Therapeutic Targets The overview of Wnt signaling pathway The overview of TGF-β signaling pathway The Encyclopedia of Common Virus PI3K-Akt Signaling Pathway and Cancer Double-Edged Sword: TGF-β Do You Really Know TGF-β Superfamily? CCR9: A Member of the Chemokine Receptor Family, an Emerging Target for Numerous Tumor Researches! Chemokine Receptor CCR6: the Specific Receptor for CCL20, a Potential Target for Drug Research in Inflammatory Diseases and Gastrointestinal Cancer! CEACAM6: a Potent CEA Family Molecule, a New Tumor Marker or Anti-Cancer Target! What Is Tomato Mosaic Virus IL2RA (CD25): A Specific Marker of Tregs Functions as Both Immune Modulators and Targeted Agents in Various Diseases! What Should You Know about Exosomes? Bispecific Antibody Overview: From Definition to Application The Review of Post-translational Modifications CXCR4: An Attractive Target of GPCR family Brings Promising New Drugs for Cancer Therapy！ CLDN3: Another Tight Junction Protein or New Drug Target, Interest in CLDN Family is Growing Fast! PROM1 (CD133): A New Cell Surface Marker of Cancer Stem Cells (CSCs), A More Significant Target for Immunotherapy! ENPP3 (CD203c): An Extracellular Enzyme, a Regulator for Nucleotide and Energy, an Indicator for Allergies, an Effective Target for RCC Therapy! ROR1, an Emerging Target for Tumor Immunotherapy SEMA4D/CD100: As an Important Immunoregulator to Improve Immunotherapy CADM1: an Immunoglobulin Superfamily Molecule, a New Tumor Suppressor Gene! Oncostatin M (OSM): A Member of IL-6 Cytokine Family, An Underestimated Player in Future Targeted Therapies? MAGEA4: a Member of the Melanoma-Associated Antigen (MAGE) Family, a Popular Target for Tumor-Specific Immune Research! In Vivo vs. In Vitro: Choosing the Right Experimental Model for Your Research Dipeptidase 3 (DPEP3): an Emerging Member of the DPEP Family, a Potential Therapeutic Target in Male Fertility or Tumor Research! IL-6: an Important Participant in the Inflammatory Cascade, One of the Most Functional Cytokines in Various Diseases Research! Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Synaptic vesicle cycle

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address