Western blot
All lanes: NUMB antibody at 0.93µg/ml
Lane 1: Mouse brain tissue
Lane 2: A431 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 71, 66, 70, 65, 56, 54, 51, 49, 38 kDa
Observed band size: 71 kDa
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Regulates clathrin-mediated receptor endocytosis. Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Gene References into Functions
The Numb-Mdm2 interaction represents a fuzzy complex mediated by a short Numb sequence encompassing its alternatively spliced exon 3 (Ex3), which is necessary and sufficient to inhibit Mdm2 and prevent p53 degradation. PMID: 29269425
High NUMB expression is associated with endometriosis. PMID: 29398419
The results provide new evidence of Numb and Gli1 on the clinical characteristics of Malignant pleural mesothelioma, which may be helpful in clinical diagnosis and targeted therapy. Further research with larger sample size is needed PMID: 30197333
Numb is associated with modulation of Notch-driven epithelial-mesenchymal transition. PMID: 29187638
Numb(-/low) prostate cancer cells were smaller and quiescent, preferentially expressed Notch and Hedgehog downstream and stem-cell-associated genes, and associated with a greater resistance to androgen-deprivation therapy PMID: 28751447
MAP17 overexpression activates Notch pathway by sequestering NUMB. High levels of MAP17 correlated with tumorsphere formation and Notch and Stem gene transcription. Its direct modification causes direct alteration of tumorsphere number and Notch and Stem pathway transcription PMID: 28153862
NUMB has a role in negatively regulating the epithelial-mesenchymal transition of triple-negative breast cancer by antagonizing Notch signaling PMID: 27506933
In the present study, we identified that the adaptor protein Numb, which is demonstrated to be a novel binding partner of NEDD4-1, plays important roles in controlling PTEN ubiquitination through regulating NEDD4-1 activity and the association between PTEN and NEDD4-1. PMID: 28437168
Numb expression is not associated with favorable prognosis in small cell lung cancer. PMID: 28060745
Data suggest that over-expression of NUMB has anti-cancer effects to prostatic cancer cells; these studies included experiments both in vitro and in vivo (xenograft experiments in nude mice). PMID: 28531799
Using patient-derived xenografts, the study shows that expansion of the cancer stem cells pool, due to altered self-renewing divisions, is also a feature of Numb-deficient human breast cancers . PMID: 28298340
Numb binds to another docking regulator, Mon1b, and is required for the recruitment of cytosolic Mon1b to the early endosomes membrane. PMID: 26987402
this study identified a novel molecular determinant of breast cancer progression, uncovering a potential oncogenic role for the NUMB6 protein in cancer cell migration and invasion, coupled to the maintenance of mesenchymal-like cells. PMID: 27302072
Low NUMB expression is associated with pancreatic cancer. PMID: 27092875
Together, our findings revealed a novel function of Numb and its likely mechanism in regulation of autophagy events. PMID: 28720501
investigations revealed that NUMB and NUMBL interacted with small GTPase Rab7 to transition ERBB2 from early to late endosome for degradation. PMID: 28067668
These findings highlight the importance of Numb and Numbl in the control of myoepithelial cell fate determination, epithelial identity, and lactogenesis PMID: 27383182
Our evidence supports a mechanism by which Numb AS is regulated in response to oncogenic signaling pathways, and contributes to activation of downstream pathways to promote tumorigenesis. PMID: 27041567
Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif. PMID: 27358480
HBeAg and its precursors promote HDM2-mediated degradation and impair transcriptional activity of p53 by interacting with NUMB, consequently contributing to hepatocellular carcinoma development. PMID: 27106262
High expression of Numb is associated with radiation resistance in pancreatic cancer. PMID: 27677287
Upregulated expression of Numb is associated with reduced radiosensitivity of lung cancer. PMID: 27476167
Radiation sensitivity of NSCLC stem cells was enhanced with the increase of Numb expression. PMID: 27310305
PNA-antimiR-182 reduced the levels of NICD, Hes1, HIF-1alpha, and p-Akt in both cell groups, while it augmented the intracellular content of FOXO1 and Numb suppressor proteins. In other words, PNA-antimiR-182-mediated upregulation of Numb was associated with downregulation of HIF-1alpha and Hes1. Consequently, downregulation of miR-182 might find therapeutical value for overcoming trastuzumab resistance PMID: 26563369
Dominant negative NUMB expression confers a high proliferative phenotype to chronic myeloid leukemia cells. PMID: 26944313
Notch1/Numb/Snail signal pathway plays an important role in the pathogenesis of adenomyosis. PMID: 26307032
Numb polymorphisms are associated with coronary artery disease among Han Chinese and Uighur Chinese in Xinjiang, China. PMID: 26415596
Methylation of the Numb promoter contributed to hepatocarcinogenesis. PMID: 26165304
results indicated that both rs2108552 and rs17781919 in the Numb gene were associated with total cholesterol level and density lipoprotein-cholesterol level in Chinese subjects. PMID: 26264115
Report alternative splicing of NUMB in colorectal cancer. PMID: 26074693
NANOG-NUMB-p53 signaling axis is an important regulatory pathway for tumor-initiating cells events in TIC self-renewal and liver tumorigenesis PMID: 26174965
Alternative splicing of Numb may provide a useful prognostic biomarker in HCC and is pharmacologically tractable PMID: 26058814
Numb/Numbl control VEGF receptor endocytosis, signaling, and recycling in endothelial cells, which promotes the angiogenic growth of blood vessels. PMID: 26069237
Using LC-MS/MS, the study reports the identification of 25 serine/threonine Numb phosphorylation sites, and a single tyrosine phosphorylation site. PMID: 25403733
Taken together, our data suggest that Numb may possibly function as a tumor suppressor involved in the carcinogenesis of clear cell renal cell carcinoma PMID: 25480416
Findings suggested NUMB-1 functions as a tumor-suppressor and serves as a prognositc biomarker for ESCC patients. PMID: 24980814
Numb specifically regulates NPC1L1-mediated cholesterol absorption both in human intestine and liver, distinct from ARH and Dab2, which selectively participate in LDLR-mediated LDL uptake. PMID: 25331956
Numb up-regulation significantly correlates with cell proliferation and poor prognosis in hepatocellular carcinoma patients. PMID: 24770339
miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes. PMID: 24302991
The study demonstrates coordinated regulation of Numb, MDM2 and p53 on cell invasion and migration in pancreatic cancer. PMID: 23881403
Identify the Notch pathway regulator NUMB as a key target of these factors. PMID: 24332178
Overexpression of Numb inhibited proliferation, promoted apoptosis and enhanced sensitivity to cisplatin, and activated caspase-9 and caspase-3 through release of cytochrome c as well as downregulation of XIAP and survivin. PMID: 23624653
The Numb modulates the paracellular permeability by affecting apical junctional complex (AJC) assembly and myosin light chain (MLC) phosphorylation. PMID: 23872314
Numb1 interacts with TRPV6 through charged residues and inhibits its activity via the regulation of protein expression. PMID: 23140583
Data indicate the Set8-Numb-p53 signaling axis as an important regulatory pathway for apoptosis and suggests a therapeutic strategy by targeting Numb methylation. PMID: 23706821
This study implies that any mutations within the NUMB gene that might influence the disease course of chronic myeloid leukemia are very rare. PMID: 22734830
Expression of stem cell regulator NUMB is not associated with any prognostic significance in acute myeloid leukemia patients. PMID: 23233047
Down-modulation of NUMB is associated with myelodysplastic syndromes and acute myeloid leukemia. PMID: 22784712
Dysregulation of Numb expression results in mislocalized Plk1 and poor centrosomal gamma-tubulin recruitment, potentially contributing to mitotic errors, aneuploidy, and cancer development. PMID: 22593191
Numb regulates glioma stem cell fate and growth by altering epidermal growth factor receptor and Skp1-Cullin-F-box ubiquitin ligase activity PMID: 22553175