NUP50 Antibody

Datasheet

Code	CSB-PA325589
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA325589(NUP50 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA325589(NUP50 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Hela cells, 293T cells, A549 cells, HT29 cells, K562 cells, Primary antibody: CSB-PA325589(NUP50 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9UKX7

Target Names

NUP50

Alternative Names

50 kDa nucleoporin antibody; NPAP60 antibody; NPAP60L antibody; Nuclear pore associated protein 60L antibody; Nuclear pore complex protein Nup50 antibody; Nuclear pore-associated protein 60 kDa-like antibody; Nucleoporin 50 antibody; Nucleoporin 50kDa antibody; Nucleoporin Nup50 antibody; Nup50 antibody; NUP50_HUMAN antibody; PRO1146 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Fusion protein of Human NUP50

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.

Gene References into Functions

Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin alpha5. PMID: 22130666
Results suggest that Npap60L and Npap60S function differently in nuclear protein import. PMID: 20016008

Subcellular Location

Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.

Tissue Specificity

Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.

Database Links

HGNC: 8065

OMIM: 604646

KEGG: hsa:10762

STRING: 9606.ENSP00000345895

UniGene: Hs.475103

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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