NUP50 Recombinant Monoclonal Antibody

NUP50 is a critical component of the nuclear pore complex, serving as a key regulator of nucleocytoplasmic transport and playing essential roles in cell cycle progression and chromatin organization. This nucleoporin facilitates the import of proteins containing classical nuclear localization signals and has been implicated in various cellular processes including mitotic spindle assembly and gene regulation, making it a valuable target for researchers investigating nuclear transport mechanisms and cell division.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human NUP50, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring your results remain comparable across experiments and publications.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis of HL-60 whole cell lysate reveals specific detection at an observed molecular weight of 58 kDa, slightly higher than the predicted 50 kDa—a shift commonly attributed to post-translational modifications such as glycosylation or phosphorylation that affect electrophoretic mobility. Immunohistochemistry testing in paraffin-embedded human colorectal cancer tissue shows clear nuclear staining patterns consistent with NUP50's known localization, while flow cytometry analysis using A431 cells demonstrates distinct positive signal separation from isotype controls, confirming utility for single-cell analysis applications.

The antibody's versatility across Western blotting, immunohistochemistry, and flow cytometry provides researchers with flexibility to examine NUP50 expression and localization through complementary approaches. Whether investigating nuclear transport dynamics, exploring nucleoporin function in disease contexts, or characterizing cell cycle-related processes, this antibody delivers the specificity and consistency essential for meaningful cell biology research.