ONECUT1 Antibody

Datasheet
Code CSB-PA880058LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9UBC0
Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate, Rat brain tissue
    All lanes: ONECUT1 antibody at 7.6μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • IHC image of CSB-PA880058LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Hepatocyte nuclear factor 6 protein (192-292AA)
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Hepatocyte nuclear factor 6 (HNF-6) (One cut domain family member 1) (One cut homeobox 1), ONECUT1, HNF6 HNF6A
Immunogen Species Human
Research Area Signal Transduction
Target Names ONECUT1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Subcellular Location Nucleus
Protein Families CUT homeobox family
Tissue Specificity Highly expressed in liver; lower expression in testis and skin.
Database Links

HGNC: 8138

OMIM: 604164

KEGG: hsa:3175

STRING: 9606.ENSP00000302630

UniGene: Hs.555318

Pathway Signaling pathways regulating pluripotency of stem cells

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