PAK3 Antibody

Code CSB-PA017407LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Mouse lung tissue, Mouse skeletal muscle tissue
    All lanes: PAK3 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 63, 61, 65 kDa
    Observed band size: 63 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA017407LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA017407LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PAK3 Polyclonal antibody
Uniprot No. O75914
Target Names PAK3
Alternative Names PAK3 antibody; OPHN3 antibody; Serine/threonine-protein kinase PAK 3 antibody; EC 2.7.11.1 antibody; Beta-PAK antibody; Oligophrenin-3 antibody; p21-activated kinase 3 antibody; PAK-3 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Serine/threonine-protein kinase PAK 3 protein (70-190AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Applications : the correlation analysis

Sample type: cells

Review: The correlation analysis of screened hub genes and LVEF. The expression levels of proteins PAK3 was demonstrated to be negatively correlated with LVEF.

By Anonymous

Target Background

Function
(From Uniprot)
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC).
Gene References into Functions
  1. The miR-193b-3p/PAK3 axis might be a potential novel therapeutic target for OC. PMID: 29169729
  2. Authors have identified mutations in PAK3, CASK, and MECP2 that likely contribute to intellectual disability, and the findings extend the spectrum of mutations and phenotypes associated with X-linked intellectual disability. PMID: 28481730
  3. This study showed that PARK3 messenger RNA levels was significantly upregulated in subjects with schizophrenia in laminar and cellular samples. PMID: 25981171
  4. This study identified predicted pathogenic, hemizygous variants on chromosome X in disease genes PAK3. PMID: 25666757
  5. Cdc42Hs binds to the effector domain of PAK3 PMID: 25109462
  6. Results show elevated PAK3 expression at both the mRNA and protein level in cJun/AP-1-over-expressing Rat1a fibroblasts, as well as in transformed human fibroblasts. PMID: 23818969
  7. p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. PMID: 21949127
  8. potential role of PAK3 in the progression of ACTH-producing thymic carcinoid PMID: 20960100
  9. PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. The MRX30 mutation might contribute to the cognitive deficits underlying this form of X-linked mental retardation. PMID: 15574732
  10. PAK3 mutations result in a specific form of X-linked mental retardation with fairly constant clinical features. PMID: 18523455
  11. PAK3 codes for a splice variant that contains a 45-bp alternatively spliced exon. The exon encodes 15 aa that are inserted in the regulatory domain. PAK3b displays a high kinase activity in starved cells that is not further stimulated by active GTPases. PMID: 12464619

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Involvement in disease Mental retardation, X-linked 30 (MRX30)
Subcellular Location Cytoplasm.
Protein Families Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
Tissue Specificity Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
Database Links

HGNC: 8592

OMIM: 300142

KEGG: hsa:5063

STRING: 9606.ENSP00000353864

UniGene: Hs.593599

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