PIAS1 Antibody

Code CSB-PA037444
Size US$166
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  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human testis tissue, human placenta tissue, Primary antibody: CSB-PA037444(PIAS1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
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Product Details

Uniprot No.
Target Names
PIAS1
Alternative Names
AR interacting protein antibody; DDXBP1 antibody; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody; DEAD/H box binding protein 1 antibody; DEAD/H box-binding protein 1 antibody; E3 SUMO-protein ligase PIAS1 antibody; GBP antibody; Gu binding protein antibody; Gu-binding protein antibody; GU/RH-II antibody; Pias1 antibody; PIAS1_HUMAN antibody; Protein inhibitor of activated STAT protein 1 antibody; Protein inhibitor of activated STAT; 1 antibody; RNA helicase II binding protein antibody; RNA helicase II-binding protein antibody; Zinc finger; MIZ-type containing 3 antibody; ZMIZ3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Synthetic peptide of Human PIAS1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
Antigen affinity purification
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form
Liquid
Tested Applications
ELISA,WB
Recommended Dilution
Application Recommended Dilution
ELISA 1:2000-1:5000
WB 1:500-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB.
Gene References into Functions
  1. Rad18, independently of its ubiquitin ligase activity, promotes DNA polymerase eta SUMOylation by facilitating its interaction with its SUMO ligase PIAS1 and is required for DNA polymerase eta function at difficult to replicate loci. PMID: 27811911
  2. PIAS1 as a key regulator of Epstein-Barr Virus lytic replication. PIAS1 acts as an inhibitor for transcription factors involved in lytic gene expression. PMID: 29262325
  3. these results indicate that PIAS1 is a positive regulator of MYC. PMID: 27239040
  4. PIAS1 is a prognostic biomarker in breast cancer PMID: 28493978
  5. PIAS1 is a determinant of poor survival and acts as a positive feedback regulator of AR signaling through enhanced AR stabilization in prostate cancer PMID: 26257066
  6. PIAS1 overexpression exacerbated mutant Huntingtin-associated phenotypes and aberrant protein accumulation PMID: 27146268
  7. HBs protein-induced hPIAS1 transcription requires TAL1, E47, MYOG, NFI, and MAPK signal pathways PMID: 27276529
  8. identified the SUMO ligase PIAS1 as a constituent PML-NB antiviral protein. This finding distinguishes a SUMO ligase that may mediate signaling events important in promyelocytic leukemia nuclear body mediated intrinsic immunity. PMID: 27099310
  9. PIAS1 enhances p300 recruitment to c-Myb-bound sites through interaction with both proteins. In addition, the E3 activity of PIAS1 enhances further its coactivation PMID: 27032383
  10. Results show that apocrine breast cancer and prostate cancer cells share a core AR cistrome and target gene signature linked to cancer cell growth, and PIAS1 plays a similar coregulatory role for AR in both cancer cell types. PMID: 26219822
  11. c-Myc is targeted to the proteasome for degradation in a SUMOylation-dependent manner, regulated by PIAS1, SENP7 and RNF4 PMID: 25895136
  12. Data demonstrate that PIAS1 interacts with TRF2 and mediates its sumoylation serving as a molecular switch that controls the level of TRF2 at telomeres. PMID: 26450775
  13. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
  14. Our data suggest that necdin suppresses PIAS1 both by inhibiting SUMO E3 ligase activity and by promoting ubiquitin-dependent degradation. PMID: 24911587
  15. the presented data indicate that PIAS1 is crucial for parental and docetaxel resistant prostate cancer cell survival PMID: 25474038
  16. Elevated PIAS1 expression was observed in breast tumor samples. PMID: 24586797
  17. PIAS1 is the E3 ligase responsible for SUMOylation of HMGN2. PMID: 24872413
  18. Further study indicated that PIAS1 interacted with IRF3 and inhibited the DNA binding activity of IRF3. PMID: 24036127
  19. Levels of STAT1 andor the protein expression of its negative regulators, PIAS1 and SOCS3, may be a good predictor of hepatitis C virus response to therapy PMID: 23472246
  20. Smad2 and PIAS1 proteins were significantly upregulated resulting in dramatically increased interactions between Smad2/4 and PIAS1 in the presence of zinc. PMID: 24052079
  21. Phosphorylation of serine residues adjacent to the PIAS1 SUMO-interacting motif favors formation of the non covalent PIAS1.SUMO.UBC9 ternary complex PMID: 24174529
  22. Data suggest that the pro-apoptotic protein Daxx specifically interacts with one or more substrates SUMOylated by PIAS1 and this interaction leads to apoptosis following UV irradiation. PMID: 22976298
  23. data suggest that PIAS1 may function as a tumor suppressor to regulate gastric cancer cell metastasis by targeting the MAPK signaling pathway PMID: 22972521
  24. MAPK-activated protein kinase-2 limits endothelial inflammation via the PIAS1 S522 phosphorylation-mediated increase in PIAS1 transrepression and SUMO ligase activity. PMID: 23202365
  25. PIAS1 is a SUMO ligase for GATA4 that differentially regulates GATA4 transcriptional activity independent of SUMO ligase activity and GATA4 sumoylation. PMID: 22539995
  26. The data reveal an important new role for PIAS1 in the regulation of cell proliferation in prostate cancer. PMID: 22449952
  27. PIAS1 negatively regulates ubiquitination of Msx1 homeoprotein independent of its SUMO ligase activity. PMID: 21717107
  28. There are differences in the PIAS3 expression from different stages of gastric precancerous conditions/lesions to GC, which may reveal a close relationship between expression reduction or loss of PIAS3 and gastric tumorigenesis. PMID: 21925039
  29. PIAS1 determines the level of JNK activity in human endometrial stromal cells , couples ROS signaling to the SUMO pathway, and promotes oxidative cell death. PMID: 21676946
  30. PIAS1 is a common partner for two cancer-related nuclear factors, c-Myb and FLASH. PMID: 21338522
  31. Data show that regulation of SATB1 sumoylation and caspase cleavage is controlled by SATB1 phosphorylation; specifically, PIAS1 interaction with SATB1 is inhibited by phosphorylation. PMID: 20351170
  32. protein inhibitor of activated Stat1 (PIAS1) interacts with the tetramerization and C-terminal regulatory domains of p53 in yeast two-hybrid analyses PMID: 11788578
  33. Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators. PMID: 11877418
  34. PIAS1 and PIASxalpha modulate the AR-dependent transactivation, which, at least in part, can be attributed to their SUMO-E3 activity toward AR. PMID: 12177000
  35. PIAS1 has a role in sumoylation of MDM2 in the cell nucleus PMID: 12393906
  36. found to strongly stimulate sumoylation of STAT1 at Lys703; results suggest a negative regulatory function for sumoylation. PMID: 12855578
  37. PIAS1 interacts with the N-terminal domain of human mineralocorticoid receptor and represses its ligand-dependent transcription. PMID: 14500761
  38. three-dimensional structure and its binding duality are discussed in conjunction with the biological functions of PIAS1 as a SUMO ligase PMID: 15133049
  39. PIAS1 is a checkpoint regulator which affects exit from G1 and G2 by sumoylation of p73. PMID: 15572666
  40. PIAS1 interacts with DNA cross-link repair SNM1A in nuclear focus formation. PMID: 15572677
  41. Results suggest that recombinant human interleukin-12 upregulates STAT-1 expression and that increased expression may be dose dependent. PMID: 15901746
  42. PIAS1 modulates transcriptional activation of smooth muscle cells marker genes through cooperative interactions with both serum response factor and class I basic helix-loop-helix proteins proteins. PMID: 16135793
  43. Pias1 binds to and sumoylates metabotropic glutamate receptor 8 PMID: 16144832
  44. In this study, we demonstrate that MEF2A undergoes sumoylation primarily at a single lysine residue (K395) both in vitro and in vivo. We also show that the nuclear E3 ligase, PIAS1, promotes sumoylation of MEF2A. PMID: 16563226
  45. PIAS1 is required for the appropriate localization and retention of Msx1 at the nuclear periphery in myoblast cells. PMID: 16600910
  46. TGF-beta rapidly suppresses IFN-gamma-driven STAT1 signaling by reducing DNA binding via promotion of STAT1--PIAS1 interactions and not inhibition of STAT1 activation. PMID: 17371985
  47. PIASy cooperates with PIAS1 to down-regulate the specificity and magnitude of NF-kappa B/STAT1-mediated gene activation. PMID: 17606919
  48. The data show that HCV NS3/4a is able to block the Jak-Stat signaling pathway at the stage of Stat-1 serine 727 phosphorylation. PMID: 18190974
  49. PIAS1 staining of the colon cancer tissue microarrays indicated a strong correlation of normal colon cells, and adenomas, with high expression of both PIAS1 and IRF-1 PMID: 19288270
  50. novel function of PIAS1 in the induction of JNK-dependent apoptosis, independent of the previously known inhibitory activity of PIAS1 in STAT-mediated gene activation. PMID: 11451946

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Subcellular Location
Nucleus speckle. Nucleus, PML body. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
Protein Families
PIAS family
Tissue Specificity
Expressed in numerous tissues with highest level in testis.
Database Links

HGNC: 2752

OMIM: 603566

KEGG: hsa:8554

STRING: 9606.ENSP00000249636

UniGene: Hs.162458

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