Product Details

Uniprot No.

Target Names

Alternative Names

E3 SUMO-protein ligase PIAS1 (EC 2.3.2.-) (DEAD/H box-binding protein 1) (E3 SUMO-protein transferase PIAS1) (Gu-binding protein) (GBP) (Protein inhibitor of activated STAT protein 1) (RNA helicase II-binding protein), PIAS1, DDXBP1

Species Reactivity

Human

Immunogen

A synthesized peptide from human PIAS1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PIAS1 functions as an E3 SUMO-protein ligase that plays a central role in regulating transcriptional activity through SUMOylation of target proteins. As a protein inhibitor of activated STAT1, PIAS1 serves as a critical negative regulator of cytokine signaling pathways, making it an important target for researchers investigating immune responses, inflammatory processes, and cancer biology. Its involvement in modulating transcription factor activity positions PIAS1 at the intersection of multiple signaling networks relevant to disease mechanisms.

This recombinant monoclonal antibody, clone 9B11, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human PIAS1, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. Affinity purification further enhances signal clarity across applications.

Validation testing demonstrates robust performance across multiple platforms. Western blot analysis detects PIAS1 in HeLa, K562, and Jurkat whole cell lysates, with the observed band at approximately 75 kDa slightly exceeding the predicted 72 kDa molecular weight—a difference likely attributable to post-translational modifications such as SUMOylation or phosphorylation events characteristic of this protein. Immunohistochemistry staining has been validated in human glioma and thyroid tissue sections using citrate buffer antigen retrieval, while immunofluorescence studies in A549 cells reveal the expected nuclear localization pattern. Flow cytometry applications have been confirmed in HeLa cells with clear separation from isotype controls.

This antibody supports researchers exploring STAT signaling regulation, SUMOylation pathways, and transcriptional control mechanisms across oncology and immunology applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB.

Gene References into Functions

Rad18, independently of its ubiquitin ligase activity, promotes DNA polymerase eta SUMOylation by facilitating its interaction with its SUMO ligase PIAS1 and is required for DNA polymerase eta function at difficult to replicate loci. PMID: 27811911
PIAS1 as a key regulator of Epstein-Barr Virus lytic replication. PIAS1 acts as an inhibitor for transcription factors involved in lytic gene expression. PMID: 29262325
these results indicate that PIAS1 is a positive regulator of MYC. PMID: 27239040
PIAS1 is a prognostic biomarker in breast cancer PMID: 28493978
PIAS1 is a determinant of poor survival and acts as a positive feedback regulator of AR signaling through enhanced AR stabilization in prostate cancer PMID: 26257066
PIAS1 overexpression exacerbated mutant Huntingtin-associated phenotypes and aberrant protein accumulation PMID: 27146268
HBs protein-induced hPIAS1 transcription requires TAL1, E47, MYOG, NFI, and MAPK signal pathways PMID: 27276529
identified the SUMO ligase PIAS1 as a constituent PML-NB antiviral protein. This finding distinguishes a SUMO ligase that may mediate signaling events important in promyelocytic leukemia nuclear body mediated intrinsic immunity. PMID: 27099310
PIAS1 enhances p300 recruitment to c-Myb-bound sites through interaction with both proteins. In addition, the E3 activity of PIAS1 enhances further its coactivation PMID: 27032383
Results show that apocrine breast cancer and prostate cancer cells share a core AR cistrome and target gene signature linked to cancer cell growth, and PIAS1 plays a similar coregulatory role for AR in both cancer cell types. PMID: 26219822
c-Myc is targeted to the proteasome for degradation in a SUMOylation-dependent manner, regulated by PIAS1, SENP7 and RNF4 PMID: 25895136
Data demonstrate that PIAS1 interacts with TRF2 and mediates its sumoylation serving as a molecular switch that controls the level of TRF2 at telomeres. PMID: 26450775
the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
Our data suggest that necdin suppresses PIAS1 both by inhibiting SUMO E3 ligase activity and by promoting ubiquitin-dependent degradation. PMID: 24911587
the presented data indicate that PIAS1 is crucial for parental and docetaxel resistant prostate cancer cell survival PMID: 25474038
Elevated PIAS1 expression was observed in breast tumor samples. PMID: 24586797
PIAS1 is the E3 ligase responsible for SUMOylation of HMGN2. PMID: 24872413
Further study indicated that PIAS1 interacted with IRF3 and inhibited the DNA binding activity of IRF3. PMID: 24036127
Levels of STAT1 andor the protein expression of its negative regulators, PIAS1 and SOCS3, may be a good predictor of hepatitis C virus response to therapy PMID: 23472246
Smad2 and PIAS1 proteins were significantly upregulated resulting in dramatically increased interactions between Smad2/4 and PIAS1 in the presence of zinc. PMID: 24052079
Phosphorylation of serine residues adjacent to the PIAS1 SUMO-interacting motif favors formation of the non covalent PIAS1.SUMO.UBC9 ternary complex PMID: 24174529
Data suggest that the pro-apoptotic protein Daxx specifically interacts with one or more substrates SUMOylated by PIAS1 and this interaction leads to apoptosis following UV irradiation. PMID: 22976298
data suggest that PIAS1 may function as a tumor suppressor to regulate gastric cancer cell metastasis by targeting the MAPK signaling pathway PMID: 22972521
MAPK-activated protein kinase-2 limits endothelial inflammation via the PIAS1 S522 phosphorylation-mediated increase in PIAS1 transrepression and SUMO ligase activity. PMID: 23202365
PIAS1 is a SUMO ligase for GATA4 that differentially regulates GATA4 transcriptional activity independent of SUMO ligase activity and GATA4 sumoylation. PMID: 22539995
The data reveal an important new role for PIAS1 in the regulation of cell proliferation in prostate cancer. PMID: 22449952
PIAS1 negatively regulates ubiquitination of Msx1 homeoprotein independent of its SUMO ligase activity. PMID: 21717107
There are differences in the PIAS3 expression from different stages of gastric precancerous conditions/lesions to GC, which may reveal a close relationship between expression reduction or loss of PIAS3 and gastric tumorigenesis. PMID: 21925039
PIAS1 determines the level of JNK activity in human endometrial stromal cells , couples ROS signaling to the SUMO pathway, and promotes oxidative cell death. PMID: 21676946
PIAS1 is a common partner for two cancer-related nuclear factors, c-Myb and FLASH. PMID: 21338522
Data show that regulation of SATB1 sumoylation and caspase cleavage is controlled by SATB1 phosphorylation; specifically, PIAS1 interaction with SATB1 is inhibited by phosphorylation. PMID: 20351170
protein inhibitor of activated Stat1 (PIAS1) interacts with the tetramerization and C-terminal regulatory domains of p53 in yeast two-hybrid analyses PMID: 11788578
Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators. PMID: 11877418
PIAS1 and PIASxalpha modulate the AR-dependent transactivation, which, at least in part, can be attributed to their SUMO-E3 activity toward AR. PMID: 12177000
PIAS1 has a role in sumoylation of MDM2 in the cell nucleus PMID: 12393906
found to strongly stimulate sumoylation of STAT1 at Lys703; results suggest a negative regulatory function for sumoylation. PMID: 12855578
PIAS1 interacts with the N-terminal domain of human mineralocorticoid receptor and represses its ligand-dependent transcription. PMID: 14500761
three-dimensional structure and its binding duality are discussed in conjunction with the biological functions of PIAS1 as a SUMO ligase PMID: 15133049
PIAS1 is a checkpoint regulator which affects exit from G1 and G2 by sumoylation of p73. PMID: 15572666
PIAS1 interacts with DNA cross-link repair SNM1A in nuclear focus formation. PMID: 15572677
Results suggest that recombinant human interleukin-12 upregulates STAT-1 expression and that increased expression may be dose dependent. PMID: 15901746
PIAS1 modulates transcriptional activation of smooth muscle cells marker genes through cooperative interactions with both serum response factor and class I basic helix-loop-helix proteins proteins. PMID: 16135793
Pias1 binds to and sumoylates metabotropic glutamate receptor 8 PMID: 16144832
In this study, we demonstrate that MEF2A undergoes sumoylation primarily at a single lysine residue (K395) both in vitro and in vivo. We also show that the nuclear E3 ligase, PIAS1, promotes sumoylation of MEF2A. PMID: 16563226
PIAS1 is required for the appropriate localization and retention of Msx1 at the nuclear periphery in myoblast cells. PMID: 16600910
TGF-beta rapidly suppresses IFN-gamma-driven STAT1 signaling by reducing DNA binding via promotion of STAT1--PIAS1 interactions and not inhibition of STAT1 activation. PMID: 17371985
PIASy cooperates with PIAS1 to down-regulate the specificity and magnitude of NF-kappa B/STAT1-mediated gene activation. PMID: 17606919
The data show that HCV NS3/4a is able to block the Jak-Stat signaling pathway at the stage of Stat-1 serine 727 phosphorylation. PMID: 18190974
PIAS1 staining of the colon cancer tissue microarrays indicated a strong correlation of normal colon cells, and adenomas, with high expression of both PIAS1 and IRF-1 PMID: 19288270
novel function of PIAS1 in the induction of JNK-dependent apoptosis, independent of the previously known inhibitory activity of PIAS1 in STAT-mediated gene activation. PMID: 11451946

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Subcellular Location

Nucleus speckle. Nucleus, PML body. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.

Protein Families

PIAS family

Tissue Specificity

Expressed in numerous tissues with highest level in testis.

Database Links

HGNC: 2752

OMIM: 603566

KEGG: hsa:8554

STRING: 9606.ENSP00000249636

UniGene: Hs.162458

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Code	CSB-RA106449A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: PIAS1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 72 kDa Observed band size: 75 kDa Exposure time：3min IHC image of CSB-RA106449A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA106449A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA106449A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA106449A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PIAS1 Recombinant Monoclonal Antibody

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