PPP2R5E Antibody

Code CSB-PA619075LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • IHC image of CSB-PA619075LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA619075LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PPP2R5E Polyclonal antibody
Uniprot No. Q16537
Target Names PPP2R5E
Alternative Names 2A5E_HUMAN antibody; Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody; PP2A B subunit B' epsilon antibody; PP2A B subunit B' epsilon isoform antibody; PP2A B subunit B56 epsilon antibody; PP2A B subunit B56 epsilon isoform antibody; PP2A B subunit isoform B''-epsilon antibody; PP2A B subunit isoform B'-epsilon antibody; PP2A B subunit isoform B56-epsilon antibody; PP2A B subunit isoform PR61-epsilon antibody; PP2A B subunit isoform R5-epsilon antibody; PP2A B subunit PR61 epsilon antibody; PP2A B subunit PR61 epsilon isoform antibody; PP2A B subunit R5 epsilon antibody; PP2A B subunit R5 epsilon isoform antibody; PPP2R5E antibody; Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody; Protein phosphatase 2 regulatory subunit B' epsilon antibody; Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody; Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform protein (2-149AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:500-1:1000
IF 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Gene References into Functions
  1. results suggest that the PPP2R5E phosphatase may contribute to microtubule organization by stabilizing MTCL1. PMID: 27521566
  2. PP2A holoenzyme containing B56 is responsible for the dephosphorylation of gamma-H2AX and regulation of DNA repair of double strand breaks induced by camptothecin. PMID: 25772433
  3. the significance of miR-23a and PPP2R5E in the proliferation and apoptosis of gastric cancer cells PMID: 24997345
  4. Downregulation of PPP2R5E affects the oncogenic potential of leukemic cells. PMID: 23812941
  5. identified the phosphorylation of hSgo2 by Aurora B at the N-terminal coiled-coil region and the middle region, and showed that these phosphorylations separately promote binding of hSgo2 to PP2A and MCAK PMID: 20889715
  6. The PPP2R5E gene is identified as harboring genetic variants that can affect human cancer and are possibly under evolutionary selection pressure. PMID: 19773383

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Subcellular Location Cytoplasm
Protein Families Phosphatase 2A regulatory subunit B56 family
Database Links

HGNC: 9313

OMIM: 601647

KEGG: hsa:5529

STRING: 9606.ENSP00000337641

UniGene: Hs.334868

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