PRICKLE3 Antibody

Code CSB-PA018678PA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate
    All lanes: PRICKLE3 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 69, 60 kDa
    Observed band size: 69 kDa

  • IHC image of CSB-PA018678PA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PRICKLE3 Polyclonal antibody
Uniprot No.
Target Names
PRICKLE3
Alternative Names
PRICKLE3 antibody; LMO6Prickle planar cell polarity protein 3 antibody; LIM domain only protein 6 antibody; LMO-6 antibody; Prickle-like protein 3 antibody; Pk3 antibody; Triple LIM domain protein 6 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Peptide sequence from Human Prickle-like protein 3 protein (9-24AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PRICKLE3 Antibody (Product code: CSB-PA018678PA01HU) is Non-conjugated. For PRICKLE3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA018678PB01HU PRICKLE3 Antibody, HRP conjugated ELISA
FITC CSB-PA018678PC01HU PRICKLE3 Antibody, FITC conjugated
Biotin CSB-PA018678PD01HU PRICKLE3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation. PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module. Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions. Involved in the organization of the basal body. Involved in cilia growth and positioning. Required for proper assembly, stability, and function of mitochondrial membrane ATP synthase (mitochondrial complex V).
Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion.
Protein Families
Prickle / espinas / testin family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 6645

OMIM: 300111

KEGG: hsa:4007

STRING: 9606.ENSP00000365494

UniGene: Hs.732712

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