PRICKLE3 Antibody

Datasheet

Code	CSB-PA018678PA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate All lanes: PRICKLE3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 60 kDa Observed band size: 69 kDa IHC image of CSB-PA018678PA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PRICKLE3 Polyclonal antibody

Uniprot No.

O43900

Target Names

PRICKLE3

Alternative Names

PRICKLE3 antibody; LMO6Prickle planar cell polarity protein 3 antibody; LIM domain only protein 6 antibody; LMO-6 antibody; Prickle-like protein 3 antibody; Pk3 antibody; Triple LIM domain protein 6 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human Prickle-like protein 3 protein (9-24AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PRICKLE3 Antibody (Product code: CSB-PA018678PA01HU) is Non-conjugated. For PRICKLE3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018678PB01HU	PRICKLE3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA018678PC01HU	PRICKLE3 Antibody, FITC conjugated
Biotin	CSB-PA018678PD01HU	PRICKLE3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation. PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module. Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions. Involved in the organization of the basal body. Involved in cilia growth and positioning. Required for proper assembly, stability, and function of mitochondrial membrane ATP synthase (mitochondrial complex V).

Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion.

Protein Families

Prickle / espinas / testin family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 6645

OMIM: 300111

KEGG: hsa:4007

STRING: 9606.ENSP00000365494

UniGene: Hs.732712

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Wnt signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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