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| Code | CSB-PA618759LC01HU |
| Size | US$299 |
| Uniprot No. | Q13131 |
| Immunogen | Recombinant Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 protein (325-543AA) |
| Raised in | Rabbit |
| Species Reactivity | Human |
| Tested Applications | ELISA |
| Relevance | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. |
| Form | Liquid |
| Conjugate | FITC |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method | >95%, Protein G purified |
| Isotype | IgG |
| Clonality | Polyclonal |
| Alias | 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) (EC 2.7.11.1) (Acetyl-CoA carboxylase kinase) (ACACA kinase) (EC 2.7.11.27) (Hydroxymethylglutaryl-CoA reductase kinase) (HMGCR kinase) (EC 2.7.11.31) (Tau-protein kinase PRKAA1) (EC 2.7.11.26), PRKAA1, AMPK1 |
| Immunogen Species | Human |
| Protocols | ELISA Protocol |
| Research Area | Neuroscience |
| Target Names | PRKAA1 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Function | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes |
| Subcellular Location | Cytoplasm, Nucleus |
| Protein Families | Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily |
| Database Links |
HGNC: 9376 OMIM: 602739 KEGG: hsa:5562 STRING: 9606.ENSP00000346148 UniGene: Hs.43322 |
| Pathway |
mTOR signaling pathway
PI3K-Akt signaling pathway Autophagy Adipocytokine signaling pathway AMPK signaling Apelin signaling pathway FoxO signaling pathway Glucagon signaling pathway Insulin signaling pathway |
PRKAA1 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA618759KA01HU | PRKAA1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IP |
| CSB-PA261465 | Phospho-PRKAA1/PRKAA2 (Ser487) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA592613 | PRKAA1/PRKAA2 (Ab-487) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA913725 | PRKAA1 (Ab-174/172) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA131259 | PRKAA1 Antibody | Human,Mouse,Rat | WB,IHC,IF,ELISA |
| CSB-PA953053 | Phospho-PRKAA1 (S496) Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA865713 | PRKAA1 Antibody | Human,Mouse,Rat | WB |
| CSB-PA801858 | PRKAA1 Antibody | Human,Mouse,Rat | WB,IHC |
| CSB-PA228254 | PRKAA1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA345903 | PRKAA1 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-PA071831 | PRKAA1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
PRKAA1 Antibodies for Human
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA618759LA01HU | PRKAA1 Antibody | Human, Mouse | ELISA, WB, IHC, IF |
| CSB-PA618759LB01HU | PRKAA1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA618759LD01HU | PRKAA1 Antibody, Biotin conjugated | Human | ELISA |
PRKAA1 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP618759HU CSB-EP618759HU CSB-BP618759HU CSB-MP618759HU |
Recombinant Human 5'-AMP-activated protein kinase catalytic subunit alpha-1(PRKAA1) | Yeast E.coli Baculovirus Mammalian cell |
2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody
Applications: ELISA, WB, ICC, IF
Species Reactivity: Human
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