PRKAA1 Antibody

Code CSB-PA618759LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: Mouse spleen tissue, MCF-7 whole cell lysate
    All lanes: AMPK1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 65, 66 kDa
    Observed band size: 65 kDa

  • Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA618759LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA618759LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA618759LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PRKAA1 Polyclonal antibody
Uniprot No.
Target Names
PRKAA1
Alternative Names
5 AMP activated protein kinase alpha 1catalytic subunit antibody; 5 AMP activated protein kinase catalytic alpha 1 chain antibody; 5' AMP activated protein kinase catalytic subunit alpha 1 antibody; 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody; AAPK1 antibody; AAPK1_HUMAN antibody; ACACA kinase antibody; acetyl CoA carboxylase kinase antibody; AI194361 antibody; AI450832 antibody; AL024255 antibody; AMP -activate kinase alpha 1 subunit antibody; AMP-activate kinase alpha 1 subunit antibody; AMP-activated protein kinase, catalytic, alpha -1 antibody; AMPK 1 antibody; AMPK alpha 1 antibody; AMPK alpha 1 chain antibody; AMPK antibody; AMPK subunit alpha 1 antibody; AMPK subunit alpha-1 antibody; AMPK1 antibody; AMPKa1 antibody; AMPKalpha1 antibody; C130083N04Rik antibody; cb116 antibody; EC 2.7.11.1 antibody; HMG CoA reductase kinase antibody; HMGCR kinase antibody; hormone sensitive lipase kinase antibody; Hydroxymethylglutaryl CoA reductase kinase antibody; im:7154392 antibody; kinase AMPK alpha1 antibody; MGC33776 antibody; MGC57364 antibody; OTTHUMP00000161795 antibody; OTTHUMP00000161796 antibody; PRKAA 1 antibody; PRKAA1 antibody; Protein kinase AMP activated alpha 1 catalytic subunit antibody; SNF1-like protein AMPK antibody; SNF1A antibody; Tau protein kinase PRKAA1 antibody; wu:fa94c10 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human 5\\\'-AMP-activated protein kinase catalytic subunit alpha-1 protein (325-543AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PRKAA1 Antibody (Product code: CSB-PA618759LA01HU) is Non-conjugated. For PRKAA1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA618759LB01HU PRKAA1 Antibody, HRP conjugated ELISA
FITC CSB-PA618759LC01HU PRKAA1 Antibody, FITC conjugated
Biotin CSB-PA618759LD01HU PRKAA1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. In that process also activates WDR45. In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
Gene References into Functions
  1. Silencing of TRPC5 and inhibition of autophagy reverses adriamycin drug resistance in breast carcinoma via CaMKKbeta/AMPKalpha/mTOR pathway. PMID: 28600513
  2. genetic inhibition of AMPK in the ventromedial nucleus of the hypothalamus (VMH) protects against high-fat diet (HFD)-induced obesity by increasing brown adipose tissue (BAT) thermogenesis and subsequently energy expenditure. PMID: 30104247
  3. Astragalus polysaccharide (APS) improved insulin sensitivity by enhancing glucose uptake, possibly through AMPK activation. These results suggested that APS might be a therapeutic candidate for insulin resistance. PMID: 30347867
  4. This case-control study provided the evidence that rs13361707CC, rs10074991GG, rs461404GG, and rs154268CC are associated with increased gastric cancer risk, especially for NCGC, and that patients with rs10074991 G or rs13361707 C allele have a poor OS. PMID: 30253744
  5. In brief, TAK1 can function as direct AMPK upstream kinase in specific contexts and in response to a subset of TAK1 activating stimuli. Further research is needed to define the intricate signals that are conditional for TAK1 to phosphorylate and activate AMPKalpha at T172. [review] PMID: 30111748
  6. Low p-AMPK expression is associated with prostate cancer. PMID: 29566977
  7. In terms of the mechanism, GL-V9 could promote the expression and activity of AMPK, leading to the decrease of G6PD and the increase of p-ACC. Thus, the level of PPP was suppressed, whereas FAO was highly enhanced. PMID: 29702405
  8. AS-IV reduced the growth, invasion, migration, and angiogenesis of lung cancer by blocking the M2 polarization of macrophages partially through the AMPK signaling pathway, which appears to play an important role in AS-IV's ability to inhibit the metastasis of lung cancer. PMID: 30157903
  9. It is an intracellular central metabolic sensor as well as a regulator, has been demonstrated to play significant roles in the contracting skeletal muscles, suggesting that AMPK should be one of the key molecules mediating metabolic effects during physical exercise. PMID: 30270274
  10. Our results showed that administration of Hcy reduced the SIRT1/AMPK/PGC-1alpha signaling in chondrocytes, leading to mitochondrial dysfunction as a result of increased oxidative stress and apoptosis PMID: 29413962
  11. Further study suggested that empagliflozin exerted its effects through inhibition of mitochondrial fission in an adenosine monophosphate (AMP)-activated protein kinase (AMPK)-dependent manner PMID: 29306791
  12. Berberine (BBR), an effective suppressor of SREBP1 and lipogenesis regulated through reactive oxygen species (ROS)/AMPK pathway, selectively inhibited the growth of G-R nonsmall cell lung cancer cells and rheumatoid arthritis patiens but not that of normal cells PMID: 28665143
  13. The knockdown of AMPK also revealed significant cytotoxicity in hypoxiamimicking conditions. These results clearly demonstrated that autophagy, especially mitophagy, was induced by the AMPK pathway when hepatocellular carcinoma cells were subjected to hypoxic conditions and played an important role in the adaptation of these cells to such conditions. PMID: 29484444
  14. Our results found that, in the mice with T2D and AD, the activators of PPARg/AMPK signaling pathway significantly increased the expression level of IDE, and decreased the accumulation of Ab40 and Ab42, as well as alleviated the spatial learning and recognition impairments. PMID: 29222348
  15. these results demonstrate that AMPK downregulation is not a triggering factor in fatty liver development but in contrast, establish the therapeutic impact of pharmacological AMPK re-activation in the treatment of fatty liver disease. PMID: 29343420
  16. AMPK played an important role in regulating cell migration, matrix contraction, and MMP production in nasal polyp-derived fibroblasts (NPDF). PMID: 29122080
  17. Proteomic analysis discovers that a novel E3 ligase, RNF44, accounts for ubiquitin-proteasome system of AMPK-alpha1 degradation in BRAF inhibitor-resistant melanoma cells. PMID: 29094484
  18. In conclusion, in the present study, mitophagy was activated and played a crucial role in cardioprotection under chronic hypoxia. AMPK was involved in mitophagy regulation, thereby providing a potential therapeutic target for heart diseases associated with chronic hypoxia. PMID: 29115402
  19. Our findings, focusing on energy balance, provide a mechanistic understanding of the promising effect of early insulin initiation on lipotoxicity. Insulin, by recovering UCP3 activity, alleviated energy surfeit and potentiated AMPK-mediated lipid homeostasis in skeletal muscle cells following exposure to PA and in gastrocnemius of mice fed HFD. PMID: 29039450
  20. identify a patient with hypotonia, weakness, delayed milestones and neurological impairment since birth harbouring a novel homozygous mutation in the AMPK catalytic alpha-subunit 1, encoded by the PRKAA1 gene. The homozygous mutation p.S487L in isoform 1 present in the patient is in a cryptic residue for AMPK activity. PMID: 29526819
  21. the present study showed that tetrandrine induced autophagy in human bladder cancer cells by regulating the AMPK/mTOR signaling pathway, which contributed to the apoptosis induction by tetrandrine, indicating that tetrandrine may be a potential anticancer candidate for the treatment of bladder cancer, and autophagy may be a possible mechanism for cancer therapy. PMID: 29048631
  22. this study found that upregulation of MACC1 in ESCC was associated with lymph node metastasis of patients, and MACC1 regulated ESCC cell proliferation, apoptosis, migration and invasion mainly through AMPK-ULK1 induced autophagy PMID: 28791376
  23. CTRP9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating PRKAA1. PMID: 28524645
  24. we have identified a novel mechanism for DIM- and ring-DIM-induced protective autophagy, via induction of AEG-1 and subsequent activation of AMPK. Our findings could facilitate the development of novel drug therapies for prostate cancer that include selective autophagy inhibitors as adjuvants. PMID: 28923415
  25. AMPK-PGC-1a control of mitochondrial reactive oxygen species regulates Warburg metabolism. PMID: 28978464
  26. Low expression of AMPK is associated with uterine cervical neoplasms. PMID: 28560405
  27. The meta-analysis reveals that the PRKAA1 rs13361707 T>C polymorphism has a significant relationship with increased gastric cancer risk. PMID: 29620653
  28. These results strongly suggest that AMPK can activate ORP150 through FOXO1 pathway and confer protection against endoplasmic reticulum stress - induced apoptosis of airway epithelial cells following exposure to cigarette smoke extract. PMID: 29448096
  29. These findings revealed that prosurvival autophagy was one of the mechanisms involved in the resistance acute myeloid leukemia (AML) leukemia stem cells to JQ1. Targeting the AMPK/ULK1 pathway or inhibition of autophagy could be an effective therapeutic strategy for combating resistance to BET inhibitors in AML and other types of cancerof cancer PMID: 27864418
  30. These findings collectively indicate that ALR negatively regulates the autophagy process through an association with the AMPK/mTOR signaling pathway. Autophagy inhibit apoptosis and play a protective role under conditions of oxidative stress. PMID: 28466106
  31. Our data indicated that miR-451 relays environmental signals by upregulating the activity of AMPK signaling, thereby modulating the activation of mTOR and Rac1/cofilin which, in turn, play key roles in glioma cell proliferation and migration, respectively. Our results highlight the need to consider opposing roles of a therapeutic target which, while suppressing tumor cell proliferation, could also promote cell infiltratio PMID: 28440461
  32. Data show that miR-135b selectively targets the AMPK phosphatase Ppm1e. PMID: 27661114
  33. We found that activation of AMPK by all fluorinated N,N-diarylureas (FND) compounds at micromolar levels significantly inhibited the cell-cycle progression and subsequent cellular proliferation. PMID: 28258165
  34. Our data suggest that AMPK regulates ATM expression and partially regulates radiosensitivity under hypoxia and nutrient starvation. The molecular mechanism underlying the induction of ATM expression by AMPK remains to be elucidated. PMID: 29284117
  35. These results suggest that berberine-induced activation of AMPK may contribute to hepatic FGF21 expression via NUR77. PMID: 29247651
  36. AMPK enhances intestinal barrier function and epithelial differentiation via promoting CDX2 expression, which is partially mediated by altered histone modifications in the Cdx2 promoter. PMID: 28234358
  37. activation of AMPK upregulated Smad6 and Smurf1 and thereby enhanced their interactions, resulting in its proteosome-dependent degradation of ALK2. PMID: 28847510
  38. Lack of mitochondrial DNA impairs chemical hypoxia-induced autophagy in liver tumor cells through reactive oxygen species-AMPK-ULK1 signaling dysregulation independently of HIF-1A. PMID: 27687210
  39. Data indicate that nesfatin-1/NUCB-2 enhanced migration, invasion and epithelial-mesenchymal transition (EMT) in colon cancer cells through LKB1/AMPK/TORC1/ZEB1 pathways in vitro and in vivo. PMID: 27150059
  40. Taken together, these results demonstrate that piperine enhance osteoblast differentiation through AMPK phosphorylation in MC3T3-E1 cells. PMID: 29203239
  41. AMP-activated protein kinase (AMPK) regulates autophagy by phosphorylating BECN1 at Thr388 PMID: 27304906
  42. activation of AMPK might be a stress response of host cells to restrict virus production through promotion of autophagic degradation PMID: 27305174
  43. The results suggest that SESN2 increases degradation of HIF-1A via AMPK-PHD regulation that contributes to inhibition of in vitro and in vivo tumorigenesis. PMID: 27840318
  44. These results demonstrate that Poly(ADP-ribosyl)ation of AMPK is a key early signal to efficiently convey extracellular nutrient perturbations with downstream events needed for the cell to optimize autophagic commitment before autophagosome formation. PMID: 27689873
  45. Data show that oxidative stress and MAP kinase phosphatase 3 (MKP3) inhibition play a critical role in procyanidin B2 3,3''-di-O-gallate (B2G2)-induced cell death in prostate cancer (PCa) cells through sustained activation of both ERK1/2 and AMPKalpha. PMID: 28876465
  46. vitamin C and edaravone effectively protected macrophages from stress-induced cytotoxicity, accompanied by downregulated SIRT3 expression and AMPK phosphorylation, and decreased level of autophagy response. Taken together, we conclude that a SIRT3/AMPK/autophagy network orchestrates in the protective effect of resveratrol in macrophages. PMID: 27021965
  47. This review discusses the current understanding of the molecular and physiological regulation of AMPK and its metabolic and physiological functions. In addition discussed are the mechanisms underlying the versatile roles of AMPK in diabetes and cancer. [review] PMID: 27416781
  48. MAGEA6 promotes glioma cell survival possibly via targeting AMPKalpha1. PMID: 29024810
  49. Depletion of glycolytic intermediates led to a consistent decrease in TXNIP expression in response to 1,25(OH)2D3, an effect that coincided with the activation of AMPK signaling and a reduction in c-MYC expression. PMID: 28651973
  50. Here, theauthors identify GIV/Girdin as a novel effector of AMPK, whose phosphorylation at a single site is both necessary and sufficient for strengthening mammalian epithelial tight junctions and preserving cell polarity and barrier function in the face of energetic stress. PMID: 27813479

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Subcellular Location
Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Database Links

HGNC: 9376

OMIM: 602739

KEGG: hsa:5562

STRING: 9606.ENSP00000346148

UniGene: Hs.43322

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