PRMT3 Antibody

Datasheet

Citations (1)

Code	CSB-PA018733LA01HU
Size	US$166
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Image	Western blot All lanes: PRMT3 antibody at 7μg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 53 kDa Observed band size: 53 kDa Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA018733LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human glioma using CSB-PA018733LA01HU at dilution of 1:100 Immunofluorescent analysis of MCF-7 cells using CSB-PA018733LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PRMT3 Polyclonal antibody

Uniprot No.

O60678

Target Names

PRMT3

Alternative Names

2010005E20Rik antibody; 2410018A17Rik antibody; AL033309 antibody; ANM3_HUMAN antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody; HMT1 hnRNP methyltransferase like 3 antibody; HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody; HMT1-like 3 antibody; hnRNP methyltransferase like 3 antibody; HRMT1L3 antibody; PRMT 3 antibody; PRMT3 antibody; Protein arginine methyltransferase 3 antibody; Protein arginine N methyltransferase 3 antibody; Protein arginine N-methyltransferase 3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein arginine N-methyltransferase 3 protein (311-529AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PRMT3 Antibody (Product code: CSB-PA018733LA01HU) is Non-conjugated. For PRMT3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018733LB01HU	PRMT3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA018733LC01HU	PRMT3 Antibody, FITC conjugated
Biotin	CSB-PA018733LD01HU	PRMT3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential. K Qiu,Cells,2020

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western Blot

Sample type: Muscle Myo-lineage cells

Review: In order to verify the reliability of proteomics data, 7 DEPs were randomly selected for Western blot analysis. As shownin FigureS1, there lativea bundance sof selected proteins between Myo-L and Myo-Y determined by Western blot were highly consistent with the data of TMT analysis.

By Anonymous

Target Background

Function

Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases. May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity.

Gene References into Functions

Findings uncover the existence of an extra-ribosomal complex consisting of PDCD2L, RPS2, and PRMT3 and support a role for PDCD2L in the late maturation of 40S ribosomal subunits. PMID: 27697862
This work profilies substrates of protein arginine N-methyltransferase 3 with S-adenosyl-L-methionine analogues. PMID: 24320160
Mutational defects in PRMT3 is not the cause of frontotemporal lobar degeneration. PMID: 23635657
results show that protein arginine methyl transferase (PRMT)-3 and -5 methylate NaV1.5 in vitro, interact with NaV1.5 in human embryonic kidney (HEK) cells, and increase NaV1.5 current density PMID: 23912080
The crystal structure of PRMT3 in complex with an inhibitor as well as kinetic analysis reveals an allosteric site of inhibition. PMID: 22795084
release of VHL30 from the E3 ligase complex, promotes the binding of VHL30 to a protein arginine methyltransferase, PRMT3 PMID: 21942715
The Tyr87Cys and Tyr87Glu-PRMT3 variants had markedly decreased affinity to ribosomal protein S2 and, consequently, reduced enzymatic activity compared to the wild-type enzyme. PMID: 21059412
PRMT3 is a ribosomal protein methyltransferase that affects the cellular level of ribosomal subunits. PMID: 15175657
DAL-1/4.1B protein significantly inhibits PRMT3 methylation of cellular substrates, which may affect mechanism through which DAL-1/4.1B affects tumor cell growth. PMID: 15334060
Type I Arginine Methyltransferases PRMT1 and PRMT-3 Act Distributively PMID: 19158082
results suggest that the pathological mutation in the PABPN1 gene alters the protein conformation and induces a preferential interaction with type I PRMTs and Hsp70 chaperones PMID: 19641605

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Subcellular Location

Cytoplasm.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family

Database Links

HGNC: 30163

OMIM: 603190

KEGG: hsa:10196

STRING: 9606.ENSP00000331879

UniGene: Hs.152337

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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