PRMT3 Antibody

Code CSB-PA018733LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: PRMT3 antibody at 7μg/ml + MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 60, 53 kDa
    Observed band size: 53 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA018733LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human glioma using CSB-PA018733LA01HU at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA018733LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PRMT3 Polyclonal antibody
Uniprot No. O60678
Target Names PRMT3
Alternative Names 2010005E20Rik antibody; 2410018A17Rik antibody; AL033309 antibody; ANM3_HUMAN antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody; HMT1 hnRNP methyltransferase like 3 antibody; HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody; HMT1-like 3 antibody; hnRNP methyltransferase like 3 antibody; HRMT1L3 antibody; PRMT 3 antibody; PRMT3 antibody; Protein arginine methyltransferase 3 antibody; Protein arginine N methyltransferase 3 antibody; Protein arginine N-methyltransferase 3 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Protein arginine N-methyltransferase 3 protein (311-529AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential.. K Qiu,Cells,2020

Applications: Western Blot
Sample type: Muscle Myo-lineage cells
Review: In order to verify the reliability of proteomics data, 7 DEPs were randomly selected for Western blot analysis. As shownin FigureS1, there lativea bundance sof selected proteins between Myo-L and Myo-Y determined by Western blot were highly consistent with the data of TMT analysis.
PMID: 32331484

Target Data

Function Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Gene References into Functions
  1. Findings uncover the existence of an extra-ribosomal complex consisting of PDCD2L, RPS2, and PRMT3 and support a role for PDCD2L in the late maturation of 40S ribosomal subunits. PMID: 27697862
  2. This work profilies substrates of protein arginine N-methyltransferase 3 with S-adenosyl-L-methionine analogues. PMID: 24320160
  3. Mutational defects in PRMT3 is not the cause of frontotemporal lobar degeneration. PMID: 23635657
  4. results show that protein arginine methyl transferase (PRMT)-3 and -5 methylate NaV1.5 in vitro, interact with NaV1.5 in human embryonic kidney (HEK) cells, and increase NaV1.5 current density PMID: 23912080
  5. The crystal structure of PRMT3 in complex with an inhibitor as well as kinetic analysis reveals an allosteric site of inhibition. PMID: 22795084
  6. release of VHL30 from the E3 ligase complex, promotes the binding of VHL30 to a protein arginine methyltransferase, PRMT3 PMID: 21942715
  7. The Tyr87Cys and Tyr87Glu-PRMT3 variants had markedly decreased affinity to ribosomal protein S2 and, consequently, reduced enzymatic activity compared to the wild-type enzyme. PMID: 21059412
  8. PRMT3 is a ribosomal protein methyltransferase that affects the cellular level of ribosomal subunits. PMID: 15175657
  9. DAL-1/4.1B protein significantly inhibits PRMT3 methylation of cellular substrates, which may affect mechanism through which DAL-1/4.1B affects tumor cell growth. PMID: 15334060
  10. Type I Arginine Methyltransferases PRMT1 and PRMT-3 Act Distributively PMID: 19158082
  11. results suggest that the pathological mutation in the PABPN1 gene alters the protein conformation and induces a preferential interaction with type I PRMTs and Hsp70 chaperones PMID: 19641605

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Subcellular Location Cytoplasm
Protein Families Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
Database Links

HGNC: 30163

OMIM: 603190

KEGG: hsa:10196

STRING: 9606.ENSP00000331879

UniGene: Hs.152337

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