PRPF40A Antibody

Datasheet

Code	CSB-PA018769LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate All lanes: PRPF40A antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 106, 107, 11 kDa Observed band size: 109 kDa IHC image of CSB-PA018769LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PRPF40A Polyclonal antibody

Uniprot No.

O75400

Target Names

PRPF40A

Alternative Names

Fas ligand associated factor 1 antibody; Fas ligand-associated factor 1 antibody; FBP 11 antibody; FBP11 antibody; FLAF 1 antibody; FLAF1 antibody; FNBP 3 antibody; Formin binding protein 11 antibody; Formin binding protein 3 antibody; Formin-binding protein 11 antibody; Formin-binding protein 3 antibody; HIP-10 antibody; HIP10 antibody; Huntingtin interacting protein A antibody; Huntingtin interacting protein HYPA antibody; Huntingtin yeast partner A antibody; Huntingtin-interacting protein 10 antibody; Huntingtin-interacting protein A antibody; HYPA antibody; NY REN 6 antibody; NY REN 6 antigen antibody; PR40A_HUMAN antibody; Pre mRNA processing factor 40 homolog A antibody; Pre-mRNA-processing factor 40 homolog A antibody; PRP40 pre mRNA processing factor 40 homolog A (S cerevisiae) antibody; PRP40 pre mRNA processing factor 40 homolog A (yeast) antibody; PRP40 pre mRNA processing factor 40 homolog A antibody; PRPF 40A antibody; PRPF40A antibody; Renal carcinoma antigen NY-REN-6 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Pre-mRNA-processing factor 40 homolog A protein (120-266AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PRPF40A Antibody (Product code: CSB-PA018769LA01HU) is Non-conjugated. For PRPF40A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018769LB01HU	PRPF40A Antibody, HRP conjugated	ELISA
FITC	CSB-PA018769LC01HU	PRPF40A Antibody, FITC conjugated
Biotin	CSB-PA018769LD01HU	PRPF40A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.

Gene References into Functions

our data revealed that COL1A1, UCP2, and PRPF40A are novel players implicated in the complex network of hypoxia response in non-small cell lung cancer PMID: 28258342
Multidisciplinary approach showed that HsPrp40Ap interacts with centrin in vitro, supporting a coupled functional role for these proteins in pre-mRNA splicing. PMID: 28636910
Study of native domain FF3-71 from human HYPA/FBP11 and the truncated version FF3-60 with C-terminal helix being deleted by molecular dynamics simulations. PMID: 22408419
Interaction with polyglutamine-expanded huntingtin alters cellular distribution and RNA processing of huntingtin yeast two-hybrid protein A (HYPA). PMID: 21566141
an atomic-resolution structure of an "invisible" folding intermediate of a small protein module: the FF domain; structure reveals non-native elements preventing formation of native conformation in the carboxyl-terminal part of the protein PMID: 20829478
Data describe the structure of an FF domain from HYPA/FBP11, which differs from those of other phosphopeptide-binding domains and represents a novel phosphopeptide-binding fold. PMID: 12381297
Results suggest an essential role of WW/FF domain-containing factors such as FBP11 and CA150 in pre-mRNA splicing that likely occurs in concert with transcription in vivo. PMID: 15456888
NAKAP-HypA scaffold is a potential nuclear docking site for huntingtin protein and may contribute to the nuclear accumulation of huntingtin observed in HD. PMID: 16391387
FBP11 binding mechanism underlies the molecular pathology of severe neurological disorders, e.g., Rett syndrome and Huntington disease. PMID: 17065151

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Subcellular Location

Nucleus speckle. Nucleus matrix.

Protein Families

PRPF40 family

Tissue Specificity

Expressed in the brain cortex (at protein level). Widely expressed.

Database Links

HGNC: 16463

OMIM: 612941

KEGG: hsa:55660

STRING: 9606.ENSP00000386458

UniGene: Hs.643580

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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