Phospho-CBL (Tyr700) Antibody

Datasheet
Code CSB-PA205538
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P22681
Image
  • Western blot analysis of extracts from HT29 cells untreated or treated with UV using c-Cbl(phospho-Tyr700) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl(Phospho-Tyr700) Antibody(left) or the same antibody preincubated with blocking peptide(right).
  • Immunofluorescence staining of methanol-fixed Hela cells using c-Cbl(phospho-Tyr700) Antibody.
Immunogen Peptide sequence around phosphorylation site of tyrosine 770 (T-E-Y(p)-M-T) derived from Human c-Cbl.
Raised in Rabbit
Species Reactivity Human
Specificity The antibody detects endogenous level of c-Cbl only when phosphorylated at tyrosine 770.
Tested Applications ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200
Relevance Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

Blake, T.J. et al. (1991) Oncogene 6, 653-657.
Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias Signal transduction protein CBL; Proto-oncogene c-CBL; Casitas B-lineage lymphoma proto-oncogene; RING finger protein 55;
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Target Names CBL
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Involvement in disease Noonan syndrome-like disorder with or without juvenile myelomonocytic leukemia (NSLL)
Subcellular Location Cytoplasm, Cell membrane
Database Links

HGNC: 1541

OMIM: 165360

KEGG: hsa:867

STRING: 9606.ENSP00000264033

UniGene: Hs.504096

Pathway ErbB signaling pathway
T cell receptor signaling pathway
Insulin signaling pathway
Endocytosis

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