Phospho-CSF1R (Tyr699) Antibody

Code CSB-PA152912
Size US$360
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  • Western blot analysis of extracts from HepG2 cells, using CSFR (Phospho-Tyr699) antibody. The lane on the right is treated with the synthesized peptide.
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CSF1R Polyclonal antibody
Uniprot No.
Target Names
CSF1R
Alternative Names
C FMS antibody; CD 115 antibody; CD115 antibody; CD115 antigen antibody; CFMS antibody; Colony stimulating factor 1 receptor antibody; Colony stimulating factor I receptor antibody; CSF 1 R antibody; CSF 1R antibody; CSF-1 receptor antibody; CSF-1-R antibody; CSF1 R antibody; CSF1R antibody; CSF1R_HUMAN antibody; CSFR antibody; EC 2.7.10.1 antibody; FIM 2 antibody; FIM2 antibody; FMS antibody; FMS proto oncogene antibody; FMS protooncogene antibody; HDLS antibody; M-CSF Receptor antibody; M-CSF-R antibody; Macrophage colony stimulating factor 1 receptor antibody; Macrophage colony stimulating factor I receptor antibody; Macrophage colony-stimulating factor 1 receptor antibody; McDonough feline sarcoma viral (v fms) oncogene homolog antibody; MCSFR antibody; Oncogen FMS antibody; Proto-oncogene c-Fms antibody; V-FMS McDonough feline sarcoma viral oncogen homolog; formerly antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 699 (V-D-Y(p)-K-N) derived from Human CSF1R.
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:3000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages.
Gene References into Functions
  1. M-CSFR inhibition suppressed programmed death-1 and -2 ligand in adult T-cell leukemia/lymphoma (ATLL) cells and macrophages stimulated with conditioned medium from ATL-T cells. PMID: 30541986
  2. The detection of the CSF1R mutation outside of the region-encoding TKD may extend the genetic spectrum of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) with CSF1R mutations. Mutational analysis of all the coding exons of CSF1R should be considered for patients clinically suspected of having ALSP. PMID: 30136118
  3. To verify its sensitivity and specificity, we retrospectively applied our criteria to 83 axonal spheroids and pigmented glia cases who had CSF1R mutations PMID: 28921817
  4. Study find elevated expression of CSF1R in primary gastric cancer tissue (GC) to be significantly associated with the presence of lymph node and peritoneal metastasis, advanced TNM stage, and poor survival. In vitro analysis also revealed a functional role for the CSF1R in GC development, and a prognostic and predictive biomarker for GC. PMID: 29767252
  5. Adult-onset Mendelian leukodystrophy genes are not common factors implicated in Alzheimer's disease, but there is a potential pathogenic link between NOTCH3, CSF1R, and sporadic late-onset Alzheimer's disease. PMID: 29544907
  6. Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia.(S39-S41) is a subtype of dominantly inherited leukoencephalopathy caused by CSF1R mutations. PMID: 28827005
  7. this is the first study to demonstrate CSF1R genetic variant regulates the CSF-1R signaling and sensitivity to CSF-1R inhibitors. PMID: 28724665
  8. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. PMID: 27602954
  9. CSF-1R is a novel therapeutic target. PMID: 27334834
  10. The phenotype of adult-onset leukoencephalopathy axonal spheroids and pigmented glia caused by CSF1R mutations is affected by sex PMID: 27680516
  11. CSFIR mutation is associated with Metaplastic Breast Cancer. PMID: 27568101
  12. Results suggest that TP63 rs7631358 G > A and CSF1R rs10079250 A > G may affect the risk and prognosis of lung cancer in never-smoking females. PMID: 28449811
  13. findings suggest that expression of wild-type CSF1R in some cells, whether achieved by mosaicism or chimerism, may confer benefit in hereditary diffuse leukoencephalopathy with axonal spheroids. PMID: 27190017
  14. This review showed that CSF1R mutation is related to Hereditary diffuse leukoencephalopathy with axonal spheroids. PMID: 27338940
  15. High CSF-1R expression is associated with Clear Cell Renal Cell Carcinoma. PMID: 26467457
  16. The aim of this study was to compare the expression of CSF-1R in nasopharyngeal carcinoma to nasopharyngitis. PMID: 26743272
  17. CSF1R mutations account for 10% of idiopathic adult onset leukodystrophies. PMID: 25935893
  18. The frequencies of the rare alleles of CCR2, ITGB3, and 3'UTR of c-fms in the Old Believers are lower than in the sample of Novosibirsk Russians, and the rare allele of DBH is more frequent PMID: 27239844
  19. Assessing serum levels of WFA(+) -CSF1R has diagnostic value for predicting carcinogenesis and the survival of LC patients. PMID: 26437001
  20. CSF1R pathway activation was evident in the cHL and inactivation of this pathway could be a potential therapeutic target in cHL cases. PMID: 26066800
  21. All 4 hereditary diffuse leukoencephalopathy with axonal spheroids patients had a different single nucleotide mutation in the cytoplasmic part of the gene. Different mutations lead to different levels of depletion of nonclassical slan-positive monocytes. PMID: 26443621
  22. results suggest that CSF1R SNP rs10079250 may contribute to lung cancer susceptibility in never-smoking females PMID: 25144241
  23. Report treatment of diffuse-type tenosynovial giant cell tumour of the soft tissue using CSF1R inhibition with emactuzumab. PMID: 26179200
  24. Autocrine CSF1R signaling is essential in maintaining low claudin expression. PMID: 25088194
  25. CSF1R gene had variations in genic regions that affected the association of RORalpha with its target binding site in vivo PMID: 25913741
  26. The first report of hereditary diffuse leukoencephalopathy with neuroaxonal spheroids due to a novel CSF1R missense mutation. PMID: 25012610
  27. A missense mutation c.2563C>A (p.P855T) of the CSF1R gene has been identified to associated with hereditary diffuse leukoencephalopathy. PMID: 25863088
  28. CSF-1R D802V and KIT D816V homologous mutations have differential effects on receptor tertiary structure and allosteric communication. PMID: 24828813
  29. C/EBPalpha-C(m)-mediated downregulation of Csf1r has a negative, rather than positive, impact on the progression of AML involving C/EBPalpha-C(m), which might possibly be accelerated by additional genetic and/or epigenetic alterations inducing Csf1r upregulation PMID: 25534203
  30. The survival of CSF1R(pos) cells requires active AKT (v-akt murine thymoma viral oncogene homolog 1) signaling, which contributed to increased levels of nuclear, transcriptionally competent beta-catenin. PMID: 24722292
  31. CSF1R gene analysis was performed in 15 patients with undefined leukoencephalopathy and progressive cognitive decline PMID: 24532199
  32. We report three patients with HDLS who carried missense mutations in the CSF1R gene, two of them novel (p.L582P and p.V383L). PMID: 24706185
  33. The identified isoform of CSF-1R mRNA may interfere with the expression of full-length CSF-1R mRNA, thereby affecting the biological activity of the ligand/receptor signaling axis in Sprague-Dawley rats. PMID: 24682770
  34. CSF-1R mayact as a transcriptional regulator on proliferation-related genes in breast cancer. PMID: 24362524
  35. Our results provide new insights into the molecular physiology of the CSF-1 receptor and contribute to our understanding of substrate selection by TACE and gamma-secretase. PMID: 24955855
  36. haploinsufficiency of CSF-1R is sufficient to cause Hereditary diffuse leukoencephalopathy with spheroids [review] PMID: 24807373
  37. CSF1R mutations in hereditary diffuse leukoencephalopathy with spheroids are loss of function. PMID: 24145216
  38. diagnosis of HDLS or a de novo mutation in CSF1R must be considered in patients with bilateral symmetric changes in ukodystrophies. PMID: 24034409
  39. Data indicate that anti-CD115 monoclonal antibody H27K15 exerts partial inhibitory effects on CD115 signaling, but inhibits monocyte chemotactic protein-1 secretion and reduces interleukin-6 production. PMID: 23924795
  40. Our data demonstrate that a high number of non-Hodgkin/Reed-Sternberg cells expressing CSF-1R are correlated with an increased tumor macrophage content and worse survival in classical Hodgkin lymphoma. PMID: 24619759
  41. Our report emphasizes the presence of atypical Parkinsonism in Hereditary diffuse leukoencephalopathy with spheroids due to CSF1R mutations PMID: 23787135
  42. our study indicates that pathogenic mutations in CSF1R are an unlikely cause of multipel sclerosis in the Canadian population PMID: 23889897
  43. This study showed that a novel A781V mutation in the CSF1R gene causes hereditary diffuse leucoencephalopathy with axonal spheroids. PMID: 23816250
  44. CSF-1R signaling by haploinsufficiency may play a role in microglial dysfunction leading to the pathogenesis of hereditary diffuse leukoencephalopathy with spheroids PMID: 24336230
  45. These results indicate that all of the Fms mutations tested severely impair the kinase activity and most of the mutations also impair the trafficking to the cell surface, further suggesting that hereditary diffuse leukoencephalopathy with spheroids is caused by the loss of Fms function. PMID: 24120500
  46. One mechanism of RANK inhibition by 1,25(OH)2D3 is down-regulation of the M-CSF receptor c-Fms, which is required for the expression of RANK. PMID: 23116709
  47. Data suggest that CSF-1R-independent actions of IL-34 via receptor-type protein-tyrosine phosphatase zeta (PTP-zeta) might be considered in evaluating IL-34 roles in development and disease. PMID: 23744080
  48. CSF1R mutations are responsible for a significant proportion of clinically and pathologically proven hereditary diffuse leukoencephalopathies with spheroids. PMID: 23649896
  49. This study showed that Adult-onset leukoencephalopathy with neuroaxonal spheroids and pigmented glia with CSF1R mutation. PMID: 23052599
  50. CSF1/CSF1R signaling is important in the recruitment of tumor-infiltrating myeloid cells that can limit the efficacy of radiotherapy PMID: 23418320

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Involvement in disease
Leukoencephalopathy, diffuse hereditary, with spheroids (HDLS)
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Tissue Specificity
Expressed in bone marrow and in differentiated blood mononuclear cells.
Database Links

HGNC: 2433

OMIM: 164770

KEGG: hsa:1436

STRING: 9606.ENSP00000286301

UniGene: Hs.586219

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