Phospho-TOB1 (Ser164) Antibody

Code CSB-PA957092
Size US$360 How to order?
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  • Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with antigen-specific peptide.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) TOB1 Polyclonal antibody
Uniprot No. P50616
Target Names TOB1
Alternative Names TOB1 antibody; TOB antibody; TROB1 antibody; Protein Tob1 antibody; Transducer of erbB-2 1 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1.
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration It differs from different batches. Please contact us to confirm it.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.
Gene References into Functions
  1. MiR-590 targets Tob1 that is significantly decreased in patients with multiple sclerosis. PMID: 28947212
  2. The dual-luciferase reporter assay also confirmed that miR-25 harbored the A allele which caused an incapacitation of binding at the TOB1. In conclusion, rs41274221 in miR-25 was a subgroup which may protect the patients from further growth and metastasis of gastric cancer and might serve as a novel biomarker for the disease PMID: 26572149
  3. A novel role for TOB1 in mediating survival of estrogen-independent breast cancers. PMID: 26165839
  4. Reduced TOB1 expression in gastric adenocarcinoma is associated with the extent of differentiation and the TNM stage of gastric cancer. PMID: 25760308
  5. miR-25 promotes GC progression by directly downregulating TOB1 expression, and may be a noninvasive biomarker for the prognosis of GC patients. PMID: 25043310
  6. arsenite-induced oxidative stress inhibits deadenylation of mRNA primarily through downregulation of Tob and Pan3, both of which mediate the recruitment of deadenylases to mRNA PMID: 25446091
  7. Tob is a key factor in the regulation of c-myc gene expression, which is essential for cell growth. PMID: 23178487
  8. TOB1 demonstrated a radioprotective function in the immortalized normal human bronchial epithelial cell line. PMID: 23756562
  9. TOB1 modulates the radiosensitivity of lung cancer cells via the MAPK/ERK signaling pathway. PMID: 23589165
  10. Cdc7 phosphorylates and interacts with Tob to inhibit the Cul4-DDB1(Cdt2)-dependent Tob degradation. PMID: 23066029
  11. study demonstrates that the overexpression of Tob1 inhibits gastric cancer progression by activating Smad4- and inhibiting betacatenin-mediated signaling pathways PMID: 22710759
  12. TOB1 overexpression not only increased the expression of the phosphatase and tensin homolog (PTEN), an important tumor suppressor, but also regulated the downstream effectors in the PI3K/PTEN signaling pathway, including Akt, ERK1/2, etc. PMID: 22158108
  13. The antiproliferative and the mRNA deadenylation/decay-promoting effects of TOB1 and TOB2 proteins are linked. PMID: 22252318
  14. Silencing of HIC1 and TOB1 expression is a common occurrence in gastric cancer and may contribute to the development and progression of the disease. PMID: 21533545
  15. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
  16. Together, these results indicate that Tob mediates the recruitment of Caf1 to the target of CPEB3 and elicits deadenylation and decay of the mRNA. PMID: 21336257
  17. Tob interactions with Caf1 and a C-terminal domain of PABPC1 PMID: 20595394
  18. functions as lateral signal transmitter PMID: 11904957
  19. Antiproliferative proteins of the BTG/Tob family are degraded by the ubiquitin-proteasome system. the C-terminal regions are necessary and sufficient to control the stabilities of BTG1, BTG2, Tob, and Tob2 proteins. PMID: 12135500
  20. identified as a MAPK substrate PMID: 12151396
  21. Decreased expression or phosphorylation status of tob protein is associated with lung cancer PMID: 14643028
  22. N-terminal region of TOB is a functional nuclear export signal. PMID: 15051490
  23. Tob is involved in the translational suppression of IL-2 mRNA in anergic T cells through its interaction with iPABP. PMID: 15676026
  24. Tob1 is a novel target for degradation by the SCF-Skp2 ubiquitin ligase in a cell line. PMID: 16951159
  25. Reverse-transcription polymerase chain reaction confirmed increased expression of GADD45A, BTG2, PDE4B, and CEBPD and downregulation of TOB1 in skeletal muscle intradialysis. PMID: 16997058
  26. Subtractive hybridization identified Twisted gastrulation (Tsg) as one of the genes suppressed by Tob. PMID: 17164348
  27. Data show that TOB enhances mRNA deadenylation in vivo, and that interaction with PABPC1 is necessary for TOB's deadenylation-enhancing effect. PMID: 17785442
  28. antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized PMID: 18084094
  29. Tob associates with the CCR4-NOT complex. PMID: 18377426
  30. positive correlation of TOB1 phosphorylation status with proliferation marker Ki67 suggests that elevated TOB1 phosphorylation might abrogate the antiproliferative effect of TOB1 in breast cancer PMID: 19491269
  31. Down-regulation of TOB is associated with breast cancer tumorigenesis. PMID: 19569230

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Subcellular Location Cytoplasm. Nucleus.
Protein Families BTG family
Tissue Specificity Ubiquitous.
Database Links

HGNC: 11979

OMIM: 605523

KEGG: hsa:10140

STRING: 9606.ENSP00000268957

UniGene: Hs.703321

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