RAD23B Antibody

Code CSB-PA019260LA01HU
Size US$166
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  • Western blot
    All lanes: RAD23B antibody at 2µg/ml
    Lane 1: 293T whole cell lysate
    Lane 2: MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 44, 36 kDa
    Observed band size: 44 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019260LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RAD23B Polyclonal antibody
Uniprot No.
Target Names
RAD23B
Alternative Names
hHR 23b antibody; hHR23B antibody; HR 23B antibody; HR23B antibody; mHR 23B antibody; mHR23B antibody; p58 antibody; RAD 23B antibody; RAD23 (S. cerevisiae) homolog B antibody; RAD23 homolog B (S. cerevisiae) antibody; RAD23 homolog B antibody; RAD23 yeast homolog of B antibody; Rad23b antibody; RD23B_HUMAN antibody; UV excision repair protein RAD23 homolog B antibody; XP C repair complementing complex 58 kDa antibody; XP C repair complementing complex 58 kDa protein antibody; XP C repair complementing protein antibody; XP-C repair-complementing complex 58 kDa protein antibody; XPC repair complementing complex 58 kDa antibody; XPC repair complementing complex 58 kDa protein antibody; XPC repair complementing protein antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human UV excision repair protein RAD23 homolog B protein (1-250AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RAD23B Antibody (Product code: CSB-PA019260LA01HU) is Non-conjugated. For RAD23B Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA019260LB01HU RAD23B Antibody, HRP conjugated ELISA
FITC CSB-PA019260LC01HU RAD23B Antibody, FITC conjugated
Biotin CSB-PA019260LD01HU RAD23B Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Applications : IHC

Sample dilution: 1:100

Review: CTCs stained with an anti-RAD23B antibody, visualized with 3,3-diaminobenzidine (DAB), and counterstained with haematoxylin ( 60 magnification).

By Anonymous

Target Background

Function
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.; Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation.; The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.
Gene References into Functions
  1. miR-196b improved radiosensitivity of SNU-638 cells by targeting RAD23B. PMID: 29864624
  2. Several mutations in the two parts of the central "crest" of the arrestin molecule, middle-loop and C-loop, enhanced or reduced arrestin-3 interactions with several GPCRs in receptor subtype and functional state-specific manner. PMID: 28473198
  3. XPC dissociation from the damage site could become a rate-limiting step in nucleotide excision repair (NER) of certain types of DNA adducts, leading to repression of NER. PMID: 27327897
  4. HR23B role in DNA reapair, in protein degradation and stability, tumorigenesis and neurodegenerative disorders [review] PMID: 27771451
  5. Data show that nucleotide excision repair factor XPC-RAD23B is a target of poly(ADP-ribosyl)ation catalyzed by poly(ADP-ribose) polymerase 1 (PARP1). PMID: 26170451
  6. RAD23B has a potential role in breast cancer progression and a tumor suppressor role of nuclear RAD23B in breast cancer. PMID: 24897598
  7. It is unlikely that the RAD23B 249Val/Val polymorphism may contribute to the individual susceptibility to cancer risk. PMID: 24643114
  8. Results define a regulatory mechanism that involves the interplay between HR23B and HDAC6 that influences the biological outcome of HDAC inhibitor treatment. PMID: 23703321
  9. Polymorphism in RAD23B gene is associated with breast cancer. PMID: 23776089
  10. HR23B expression was associated with disease stabilization for patients with unresectable hepatocellular carcinoma treated with epigenetic therapy using belinostat, a histone deacetylase inhibitor. PMID: 22915658
  11. Single nucleotide polymorphisms of CCND2, RAD23B, GRP78, CEP164, MDM2, and ALDH2 genes were significantly associated with development and recurrence of hepatocellular carcinoma in Japanese patients with hepatitis C virus. PMID: 22004425
  12. PNGase-PUB serves not only as p97-binding module but also as a possible activator of HR23 in endoplasmic reticulum-associated degradation mechanisms. PMID: 22575648
  13. 26S proteasomes and p97/VCP complexes bind to the ubiquitin-like domain of HHR23B. PMID: 19182904
  14. Gene expression of RAD23B was down-regulated during early apoptosis in human hepatoma cells exposed to Paeoniae Radix extract in vitro. PMID: 12215374
  15. analysis of mHR23A/B double-mutant cells showed that HR23 proteins function in nucleotide excision repair by governing xeroderma pigmentosum group C protein stability via partial protection against proteasomal degradation PMID: 12815074
  16. Highly expressed in the human testis and in ejaculated spermatozoa. This novel alternative splicing form of RAD23B is correlated with human spermatogenesis. PMID: 15064313
  17. the human nucleotide excision repair gene, hHR23B, is epigenetically silenced in interleukin-6-responsive multiple myeloma KAS-6/1 cells PMID: 15550378
  18. Results describe the solution structure of a protein fragment containing amino acids 275-342 of hHR23B and compare it with the previously reported solution structures of the corresponding domain of hHR23A. PMID: 15885096
  19. intracellular distribution hHR23B is cell cycle dependent PMID: 16253613
  20. determined that hHR23A and hHR23B could be co-purified with unique proteolytic and stress-responsive factors from human breast cancer tissues, indicating that they have unique functions in vivo PMID: 16712842
  21. Fluorescence correlation spectroscopy of the binding of nucleotide excision repair protein XPC-HHR23B with DNA substrates is reported. PMID: 18574675
  22. Genetic polymorphisms in RAD23B is associated with Laryngeal cancer risk associated with smoking and alcohol consumption. PMID: 19444904
  23. XPC subunit interaction with DNA is stimulated by endogenous HR23B. PMID: 19538122
  24. BRG1 stimulates the recruitment of XPG and PCNA to successfully culminate the nucleotide excision repair. PMID: 19740755

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Subcellular Location
Nucleus. Cytoplasm. Note=The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S-phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin.
Protein Families
RAD23 family
Database Links

HGNC: 9813

OMIM: 600062

KEGG: hsa:5887

STRING: 9606.ENSP00000350708

UniGene: Hs.521640

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