Product Details

Uniprot No.

Target Names

RAD51AP1

Alternative Names

2510006L10Rik antibody; MGC156698 antibody; OTTMUSP00000028651 antibody; PIR51 antibody; R51A1_HUMAN antibody; RAB22 antibody; RAD51 associated protein 1 antibody; RAD51 interacting protein antibody; RAD51-associated protein 1 antibody; RAD51-interacting protein antibody; Rad51ap1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Human RAD51AP1

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination. Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination. May also bind RNA; additional evidences are however required to confirm RNA-binding in vivo.

Gene References into Functions

RAD51AP1 upregulation is described in both tissue and matching blood from patients with ovarian and lung cancer compared with healthy controls. PMID: 29126163
The current understanding of the biochemical and biological functions of RAD51AP1 as HR factor is reviewed. It is also discussed how RAD51AP1 may help to promote cancer development and potentially be a promising new target for therapeutic intervention. [REVIEW] PMID: 28963981
USP1-UAF1 complex promotes homologous recombination repair via multiple mechanisms: through FANCD2 deubiquitination, as well as by interacting with RAD51AP1. PMID: 27463890
RAD51AP1 is required for the maintenance of DNA replication fork progression. PMID: 25288561
Data suggest that microRNA/mRNA pairs in hsa-miR-140-3p/RAD51AP1/, hsa-miR-145/E2F3, hsa-miR-139-5p/TOP2A, and hsa-miR-133a/GCLC were correlated with ovarian tumorigenesis. PMID: 22452920
We have finely mapped the two DNA binding domains in RAD51AP1 and using mutant variants, reveal that both domains are indispensable for RAD51AP1 function in cells. PMID: 22375013
RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif. PMID: 21903585
RAD51AP1 foci colocalize with a subset of DMC1 foci in spermatocytes. PMID: 21307306
Although PALB2 alone stimulates D-loop formation, it has a cooperative effect with RAD51AP1, an enhancer of RAD51. PMID: 20871616
expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues PMID: 14966907
RAD51AP1 has a selective affinity for branched-DNA structures that are obligatory intermediates during joint molecule formation. PMID: 17996710
RAD51AP1 helps maintain genomic integrity via RAD51 recombinase enhancement. PMID: 17996711
Enhanced expression of RAD51 associating protein-1 is involved in the growth of intrahepatic cholangiocarcinoma PMID: 18316552

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Subcellular Location

Chromosome. Nucleus. Chromosome, telomere.

Tissue Specificity

Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.

Database Links

HGNC: 16956

OMIM: 603070

KEGG: hsa:10635

STRING: 9606.ENSP00000228843

UniGene: Hs.730696

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Code	CSB-PA019264GA01HU
Size	$600
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RAD51AP1 Antibody

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Target Background