SHARPIN Antibody

Datasheet

Code	CSB-PA867119LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, LO2 whole cell lysate All lanes: SHARPIN antibody at 5.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 34 kDa Observed band size: 40 kDa IHC image of CSB-PA867119LA01HU diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA867119LA01HU diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of PC-3 cells with CSB-PA867119LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SHARPIN Polyclonal antibody

Uniprot No.

Q9H0F6

Target Names

SHARPIN

Alternative Names

DKFZp434N1923 antibody; hSIPL1 antibody; Shank associated RH domain interacting protein antibody; SHANK associated RH domain interactor antibody; Shank interacting protein like 1 antibody; Shank-associated RH domain-interacting protein antibody; Shank-interacting protein-like 1 antibody; Sharpin antibody; SHRPN_HUMAN antibody; SIPL1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Sharpin protein (82-171AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SHARPIN Antibody (Product code: CSB-PA867119LA01HU) is Non-conjugated. For SHARPIN Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA867119LB01HU	SHARPIN Antibody, HRP conjugated	ELISA
FITC	CSB-PA867119LC01HU	SHARPIN Antibody, FITC conjugated
Biotin	CSB-PA867119LD01HU	SHARPIN Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

Gene References into Functions

Sharpin-Arp2/3 interaction promotes lamellipodium formation. PMID: 28775156
SIPL1 contributes to promote resistance to tamoxifen in Breast cancer cells through both AKT and NF-kappaB actions. PMID: 29248549
the present study found that loss of the NEMO-SHARPIN interaction impaired recruitment of truncated NEMO forms into punctuate structures that are transiently formed on cell stimulation and thus led to a defect in linear ubiquitination PMID: 28249776
Overexpression of SHARPIN in Prostate cancer cells promoted cell growth and reduced apoptosis through NF-kB/ERK/Akt pathway and apoptosis-associated proteins. SHARPIN enhances the metastasis of prostate cancer and impair patient survivals. PMID: 28230260
Our study firstly identifies the role of SHARPIN in promoting wild-type P53 degradation and correlates with poor prognosis in P53 wild-type breast cancer. PMID: 28063307
Data show that SHANK-associated RH domain interacting protein (SHARPIN) gene expression in breast cancer patients predicts clinical outcomes. PMID: 26506596
the roles of SHARPIN in inhibiting integrin activity and supporting linear ubiquitination are (molecularly) distinct. PMID: 26600301
progesterone significantly reduced SIPL1 mRNA and protein expression in MCF7 cells. As progesterone enhances breast cancer tumorigenesis in context dependent manner, inhibition of SIPL1 expression may contribute to progesterone's non-tumorigenic function PMID: 25992689
SIPL1 binds PTEN and enhances PTEN polyubiquitination. PMID: 25152374
Sharpin deficiency sensitized primary murine keratinocytes, human keratinocytes, and mouse embryonic fibroblasts to TNF-induced apoptosis. PMID: 25443631
SIPL1 promotes AKT activation by decreasing the amount of PTEN protein in CHO-K1 cells. PMID: 25018115
SHARPIN controls lymphocyte migration by endogenously maintaining LFA-1 inactive to allow adjustable detachment of the uropods in polarized cells. PMID: 24210817
crystals of SHARPIN belonged to the primitive tetragonal space group P4(3)2(1)2, with unit-cell parameters a = b = 61.55, c = 222.81 A PMID: 22750873
the crystal structure of the N-terminal portion of SHARPIN, which adopts the highly conserved pleckstrin homology superfold that is often used as a scaffold to create protein interaction modules PMID: 22549881
SHARPIN inhibits the critical switching of beta1-integrins from inactive to active conformations. PMID: 21947080
SHARPIN is an additional component of LUBAC; SHARPIN-containing complexes can linearly ubiquitinate NEMO and activated NF-kappaB PMID: 21455180
Data report the identification of the related proteins Sipl1 (Shank-interacting protein-like 1) and Rbck1 (RBCC protein interacting with PKC1) as novel interaction partners of Eya1. PMID: 20956555
Suggest that Sharpin is not an inert scaffold protein, but may play tumor-associated roles during cancer biogenesis. PMID: 20179993

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Subcellular Location

Cytoplasm, cytosol. Cell junction, synapse.

Tissue Specificity

Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver,

Database Links

HGNC: 25321

OMIM: 611885

KEGG: hsa:81858

STRING: 9606.ENSP00000381698

UniGene: Hs.529755

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Related pathways

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