SMAD4 Antibody

Datasheet

Code	CSB-PA780020
Size	US$166
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Image	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA780020(SMAD4 Antibody) at dilution 1/20. (Original magnification: ×200) The image is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA780020(SMAD4 Antibody) at dilution 1/20. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: CSB-PA780020(SMAD4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
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Product Details
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Target Background

Product Details

Uniprot No.

Q13485

Target Names

SMAD4

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein of Human SMAD4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Involvement in disease

Pancreatic cancer (PNCA); Juvenile polyposis syndrome (JPS); Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT); Colorectal cancer (CRC); Myhre syndrome (MYHRS)

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Dwarfin/SMAD family

Database Links

HGNC: 6770

OMIM: 114500

KEGG: hsa:4089

STRING: 9606.ENSP00000341551

UniGene: Hs.75862

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Related pathways

Hippo signaling pathway TGF-beta signaling pathway Wnt signaling pathway Adherens junction Cell cycle Signaling pathways regulating pluripotency of stem cells Th17 cell differentiation AGE-RAGE signaling pathway in diabetic complications Apelin signaling pathway FoxO signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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