SMARCD2 Antibody

Datasheet

Code	CSB-PA846114LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Raw264.7 whole cell lysate All lanes: SMARCD2 antibody at 4.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 58, 56 kDa Observed band size: 59 kDa IHC image of CSB-PA846114LA01HU diluted at 1:500 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SMARCD2 Polyclonal antibody

Uniprot No.

Q92925

Target Names

SMARCD2

Alternative Names

SMARCD2 antibody; BAF60B antibody; PRO2451 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 antibody; 60 kDa BRG-1/Brm-associated factor subunit B antibody; BRG1-associated factor 60B antibody; BAF60B antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 protein (1-113AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SMARCD2 Antibody (Product code: CSB-PA846114LA01HU) is Non-conjugated. For SMARCD2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA846114LB01HU	SMARCD2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA846114LC01HU	SMARCD2 Antibody, FITC conjugated
Biotin	CSB-PA846114LD01HU	SMARCD2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation.

Gene References into Functions

Data show that both CEBPE and SMARCD2 loss-of-function mutations identified in patients with neutrophil-specific granule deficiency (SGD) abolish the interaction with SWI/SNF and secondary granule gene expression, thus providing a molecular basis for this disease. PMID: 28369034
Defective expression of SMARCD2 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily D, member 2) leads to transcriptional and chromatin changes in acute myeloid leukemia (AML) promyelocytic cells, suggesting SMARCD2 is a key factor controlling myelopoiesis and is a potential tumor suppressor in leukemia. PMID: 28369036
the Rac- and Unkempt-dependent process leading to BAF60b ubiquitination takes place in the nuclear compartment PMID: 20148946

Involvement in disease

Specific granule deficiency 2 (SGD2)

Subcellular Location

Nucleus.

Protein Families

SMARCD family

Tissue Specificity

Isoform 2 is expressed in the pancreas.

Database Links

HGNC: 11107

OMIM: 601736

KEGG: hsa:6603

STRING: 9606.ENSP00000392617

UniGene: Hs.250581

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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